Invention Application
- Patent Title: GERANYLGERANYL PYROPHOSPHATE SYNTHASES
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Application No.: US18940288Application Date: 2024-11-07
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Publication No.: US20250059519A1Publication Date: 2025-02-20
- Inventor: Viktor Marius BOER , Priscilla ZWARTJENS , Johannes Gustaaf Ernst VAN LEEUWEN
- Applicant: DSM IP ASSETS B.V.
- Applicant Address: NL Heerlen
- Assignee: DSM IP ASSETS B.V.
- Current Assignee: DSM IP ASSETS B.V.
- Current Assignee Address: NL Heerlen
- Priority: EP16196095.0 20161027
- Main IPC: C12N9/10
- IPC: C12N9/10 ; C12N9/02 ; C12N9/88 ; C12N15/52 ; C12P7/42 ; C12P15/00 ; C12P19/56

Abstract:
The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.
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