中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

28 records (took 0.037 seconds)

    GERANYLGERANYL PYROPHOSPHATE SYNTHASES
    1.
    发明申请
    GERANYLGERANYL PYROPHOSPHATE SYNTHASES 有权

    公开(公告)号：US20250059519A1

    公开(公告)日：2025-02-20

    申请号：US18940288

    申请日：2024-11-07

    Applicant: DSM IP ASSETS B.V.

    Inventor： Viktor Marius BOER ,  Priscilla ZWARTJENS ,  Johannes Gustaaf Ernst VAN LEEUWEN

    IPC: C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12N15/52 ,  C12P7/42 ,  C12P15/00 ,  C12P19/56

    Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

    KAURENOIC ACID HYDROXYLASES
    3.
    发明申请
    KAURENOIC ACID HYDROXYLASES 审中-公开

    公开(公告)号：US20200032225A1

    公开(公告)日：2020-01-30

    申请号：US16467087

    申请日：2017-12-04

    Applicant: DSM IP Assets B.V.

    Inventor： Johannes Gustaaf Ernst VAN LEEUWEN ,  Priscilla ZWARTJENS ,  Viktor Marius BOER

    IPC: C12N9/02 ,  C12P19/12 ,  A23L27/30

    Abstract: The present invention relates a polypeptide having kaurenoic acid 13-hydroxylase activity, which polypeptide comprises an amino acid sequence which, when aligned with a kaurenoic acid 13-hydroxylase comprising the sequence set out in SEQ ID NO: 1 or SEQ ID NO: 3, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions 136, 248, 336 or 403, said positions being defined with reference to SEQ ID NO: 1 or SEQ ID NO: 3 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having kaurenoic acid 13-hydroxylase activity. A polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

    EXTRACELLULAR DITERPENE PRODUCTION
    4.
    发明申请
    EXTRACELLULAR DITERPENE PRODUCTION 审中-公开
    细菌生产

    公开(公告)号：US20160102331A1

    公开(公告)日：2016-04-14

    申请号：US14893284

    申请日：2014-06-02

    Applicant: DSM IP ASSETS B.V.

    Inventor： Viktor Marius BOER ,  Nicolette Jasmijn BROERS ,  Adam G. LAWRENCE

    IPC: C12P19/56 ,  C12N9/90 ,  A23L2/60 ,  C12N9/02 ,  A23L1/236 ,  A23K1/16 ,  C07H15/256 ,  C12N9/88

    CPC classification number: C12P19/56 ,  A23K20/105 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C07H15/256 ,  C12N9/0042 ,  C12N9/0073 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P15/00 ,  C12Y114/13 ,  C12Y114/13078 ,  C12Y402/03019 ,  C12Y505/01013

    Abstract: The present invention relates to a method for the production of a diterpene or a glycosylated diterpene, which method comprises: a. fermenting a recombinant microorganism in a suitable fermentation medium, wherein the microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity and whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol, whereby a diterpene or glycosylated diterpene is produced extracellularly in the fermentation medium; and b. recovering the diterpene or glycosylated diterpene from the fermentation medium.

    Abstract translation: 本发明涉及二萜或糖基二萜的制备方法，该方法包括：a。 在合适的发酵培养基中发酵重组微生物，其中所述微生物包含一个或多个核苷酸序列，所述核苷酸序列编码：具有入 - copalyl焦磷酸合酶活性的多肽; 具有ent-Kaurene合酶活性的多肽; 具有ent-Kaurene氧化酶活性的多肽; 和具有山梨酸13-羟化酶活性的多肽，由此所述核苷酸序列的表达赋予所述微生物至少产生甜菊醇的能力，从而在发酵培养基中细胞外产生二萜或糖基化二萜; 和b。 从发酵培养基中回收二萜或糖基化二萜。

    STEVIOL GLYCOSIDE TRANSPORT
    5.
    发明申请
    STEVIOL GLYCOSIDE TRANSPORT 有权

    公开(公告)号：US20240400623A1

    公开(公告)日：2024-12-05

    申请号：US18809132

    申请日：2024-08-19

    Applicant: DSM IP ASSETS B.V.

    Inventor： Johannes Gustaaf Ernst VAN LEEUWEN ,  Viktor Marius BOER ,  Priscilla ZWARTJENS ,  Jos VAN VUGT ,  René Marcel DE JONG

    IPC: C07K14/39 ,  C12N9/02 ,  C12P19/56

    Abstract: The disclosure provides a recombinant cell capable of producing a steviol glycoside, wherein the cell comprises a nucleic acid coding for a variant of a parent polypeptide, wherein the variant has steviol glycoside transport mediating activity, wherein the variant comprises an amino acid sequence which, when aligned with the amino acid sequence of the parent polypeptide, comprises at least one modification of the amino acid residue corresponding to any of the amino acids in the amino acid sequence of the parent polypeptide, wherein the variant has an improved ability to produce rebaudioside M and optionally other steviol glycosides extracellularly if compared with the parent polytpeptide when measured under the same conditions.

    GERANYLGERANYL PYROPHOSPHATE SYNTHASES
    6.
    发明公开
    GERANYLGERANYL PYROPHOSPHATE SYNTHASES 审中-公开

    公开(公告)号：US20240067938A1

    公开(公告)日：2024-02-29

    申请号：US18455969

    申请日：2023-08-25

    Applicant: DSM IP ASSETS B.V.

    Inventor： Viktor Marius BOER ,  Priscilla ZWARTJENS ,  Johannes Gustaaf Ernst VAN LEEUWEN

    IPC: C12P19/56 ,  C12N9/10

    CPC classification number: C12P19/56 ,  C12N9/1085 ,  C12Y205/01001

    Abstract: The present invention relates a variant polypeptide having geranylgeranyl pyrophosphate synthase activity, which variant polypeptide comprises an amino acid sequence which, when aligned with a geranylgeranyl pyrophosphate synthase comprising the sequence set out in SEQ ID NO: 1, comprises at least one substitution of an amino acid residue corresponding to any of amino acids at positions 92, 100 or 235 said positions being defined with reference to SEQ ID NO: 1 and wherein the variant has one or more modified properties as compared with a reference polypeptide having geranylgeranyl pyrophosphate synthase activity. A variant polypeptide of the invention may be used in a recombinant host for the production of steviol or a steviol glycoside.

    UDP-GLYCOSYLTRANSFERASES
    7.
    发明申请
    UDP-GLYCOSYLTRANSFERASES 有权

    公开(公告)号：US20210147815A1

    公开(公告)日：2021-05-20

    申请号：US17159962

    申请日：2021-01-27

    Applicant: DSM IP ASSETS B.V.

    Inventor： Viktor Marius BOER ,  Johannes Gustaaf Ernst VAN LEEUWEN ,  Priscilla ZWARTJENS ,  Catharina Petronella Antonia KOLEN

    IPC: C12N9/10 ,  A23L27/30 ,  A23L2/60 ,  C12P5/00 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P19/56

    Abstract: The present invention relates to a recombinant host comprising a recombinant nucleic acid sequence encoding a polypeptide having at least about: a. 85% identity to the amino acid sequence set forth in SEQ ID NO: 1; b. 85% identity to the amino acid sequence set forth in SEQ ID NO: 3; c. 85% identity to the amino acid sequence set forth in SEQ ID NO: 6; d. 85% identity to the amino acid sequence set forth in SEQ ID NO: 9; e. 85% identity to the amino acid sequence set forth in SEQ ID NO: 11; f. 85% identity to the amino acid sequence set forth in SEQ ID NO: 14; g. 85% identity to the amino acid sequence set forth in SEQ ID NO: 17; h. 85% identity to the amino acid sequence set forth in SEQ ID NO: 20; i. 85% identity to the amino acid sequence set forth in SEQ ID NO: 22; or j. 85% identity to the amino acid sequence set forth in SEQ ID NO: 25.

    STEVIOL GLYCOSIDE TRANSPORT
    10.
    发明申请
    STEVIOL GLYCOSIDE TRANSPORT 有权

    公开(公告)号：US20220110350A1

    公开(公告)日：2022-04-14

    申请号：US17512368

    申请日：2021-10-27

    Applicant: DSM IP ASSETS B.V.

    Inventor： Viktor Marius BOER ,  Priscilla ZWARTJENS ,  Eric VAN DEN BERG

    IPC: A23L27/30 ,  A23L2/60 ,  C12P19/56 ,  C12P15/00 ,  C07K14/39 ,  C07K14/395 ,  C07K14/40 ,  C12N1/16 ,  C12N9/24

    Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.

Patent Agency Ranking