发明授权
US4778761A Recombinant plasmid inserted with hepatitis B virus gene, yeast
transformed with said recombinant plasmid, and production of hepatitis
B virus surface antigen
失效
插入乙型肝炎病毒基因的重组质粒，用所述重组质粒转化的酵母，并产生乙型肝炎病毒表面抗原
- 专利标题： Recombinant plasmid inserted with hepatitis B virus gene, yeast transformed with said recombinant plasmid, and production of hepatitis B virus surface antigen
- 专利标题（中）： 插入乙型肝炎病毒基因的重组质粒，用所述重组质粒转化的酵母，并产生乙型肝炎病毒表面抗原
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申请号： US4424申请日： 1987-01-20
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公开(公告)号： US4778761A公开(公告)日： 1988-10-18
- 发明人: Atsushi Miyanohara , Chikateru Nozaki , Fukusaburo Hamada , Akio Toh-e , Nobuya Ohtomo , Kenichi Matsubara
- 申请人： Atsushi Miyanohara , Chikateru Nozaki , Fukusaburo Hamada , Akio Toh-e , Nobuya Ohtomo , Kenichi Matsubara
- 申请人地址： JPX Kumamoto
- 专利权人： Juridical Foundation the Chemosero-Therapeutic Research Institute
- 当前专利权人： Juridical Foundation the Chemosero-Therapeutic Research Institute
- 当前专利权人地址： JPX Kumamoto
- 优先权： JPX57-142460 19820816
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; A61K39/29 ; C07H21/04 ; C07K14/02 ; C12N15/00 ; C12N15/62 ; C12N15/80 ; C12N15/81 ; C12P21/00 ; C12P21/02 ; C12R1/19 ; C12R1/865 ; C12N1/00
摘要：
A recombinant plasmid inserted with Hepatitis B virus gene, which comprises a plasmid vector containing a yeast gene and an E. coli gene and carrying the expression control region of the repressible acid phosphatase gene of yeast and a Hepatitis B virus gene recombined thereto under control of the phosphatase promoter, a transformed yeast which is prepared by transforming a yeast with the recombinant plasmid, and a method of the production of Hepatitis B virus surface antigen in a large scale by culturing the transformed yeast in a medium. The Hepatitis B virus surface antigen prepared by the present invention has the same immunological properties as those of the natural antigen from human blood plasma and is useful for the preparation of Hepatitis B virus vaccine and diagnostic reagents.
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