公开(公告)号：US5374625A

公开(公告)日：1994-12-20

申请号：US731459

申请日：1991-07-17

申请人： Yuh-ichiro Ichikawa ,  Hiroshi Akaba ,  Yuka Sugawara ,  Akira Shiozawa ,  Kenichi Matsubara ,  Takemitsu Nagahata ,  Hiroo Hoshino ,  Jun-ichi Seki

发明人： Yuh-ichiro Ichikawa ,  Hiroshi Akaba ,  Yuka Sugawara ,  Akira Shiozawa ,  Kenichi Matsubara ,  Takemitsu Nagahata ,  Hiroo Hoshino ,  Jun-ichi Seki

IPC分类号： C07D239/54 ,  C07D473/00 ,  A01N43/04 ,  A61K31/70 ,  C07H19/00

CPC分类号： C07D239/54 ,  C07D473/00

摘要： A novel nucleic acid derivative represented by the following general formula (I) and physiologically acceptable salt thereof which are expected to have an antiviral activity: ##STR1## wherein B represents a nucleic acid base derivative; A.sup.1 and A.sup.2 represent, independently of each other, OR.sup.1 or OCOR.sup.1 ; R.sup.1 represents a hydrogen atom, a substituted or unsubstituted alkyl group or a substituted or unsubstituted aryl group; l represents a number of 0 or 1; and m and n each represents an integer of 0-2; provided that when l and m are 0, n is 0 or 2.

摘要翻译： 预期具有抗病毒活性的由以下通式（I）表示的新型核酸衍生物及其生理学上可接受的盐：其中B表示核酸碱基衍生物; A1和A2彼此独立地表示OR1或OCOR1; R1表示氢原子，取代或未取代的烷基或取代或未取代的芳基; l表示0或1的数; m和n各自表示0-2的整数; 条件是当l和m为0时，n为0或2。

公开(公告)号：US5324730A

公开(公告)日：1994-06-28

申请号：US704085

申请日：1991-05-22

申请人： Yuh-ichiro Ichikawa ,  Hiroshi Akaba ,  Yuka Sugawara ,  Hideo Sugimura ,  Kazuhisa Narita ,  Akira Shiozawa ,  Kouwa Yamashita ,  Sayuri Kato ,  Yukihiro Nishiyama ,  Kenichi Matsubara ,  Takemitsu Nagahata

发明人： Yuh-ichiro Ichikawa ,  Hiroshi Akaba ,  Yuka Sugawara ,  Hideo Sugimura ,  Kazuhisa Narita ,  Akira Shiozawa ,  Kouwa Yamashita ,  Sayuri Kato ,  Yukihiro Nishiyama ,  Kenichi Matsubara ,  Takemitsu Nagahata

IPC分类号： C07D473/00 ,  C07F9/6558 ,  C07F9/6561 ,  A61K31/52 ,  C07D473/18 ,  C07D473/32 ,  C07F9/09

CPC分类号： C07D473/00 ,  C07F9/65583 ,  C07F9/6561 ,  C07F9/65616

摘要： This invention relates to cyclobutane derivatives represented by the following general formula (1) and physiologically acceptable salts thereof: ##STR1## wherein B represents a nucleic acid base derivative, R.sup.1 and R.sup.2 independently represent hydrogen atom, dialkylaminoacyl group, 1,4-dihydro-1-methylnicotinoyl group or substituted phosphoric acid group, provided that either one of R.sup.1 and R.sup.2 is a group other than hydrogen atom.The compounds of this invention exhibit a high oral absorbability and are metabolized in vivo into the compounds of formula (1a). Accordingly, the compounds of this invention are expectedly useful as antiviral agent.

摘要翻译： 本发明涉及由以下通式（1）表示的环丁烷衍生物及其生理学上可接受的盐：其中B表示核酸碱基衍生物，R1和R2独立地表示氢原子，二烷基氨基酰基，1,4 - 二氢-1-甲基烟酰基或取代的磷酸基，条件是R 1和R 2中的任一个是除氢原子之外的基团。 本发明的化合物表现出高的口服吸收性，并且在体内被代谢为式（1a）的化合物。 因此，预期本发明的化合物可用作抗病毒剂。

公开(公告)号：US5214048A

公开(公告)日：1993-05-25

申请号：US439270

申请日：1989-11-20

申请人： Nobuyoshi Shimada ,  Shigeru Hasegawa ,  Takayuki Tomizawa ,  Seiichi Saito ,  Kyoichi Shibuya ,  Akio Fujii ,  Hiroo Hoshino ,  Kenichi Matsubara ,  Takemitsu Nagahata ,  Katsutoshi Takahashi ,  Yukihiro Nishiyama

发明人： Nobuyoshi Shimada ,  Shigeru Hasegawa ,  Takayuki Tomizawa ,  Seiichi Saito ,  Kyoichi Shibuya ,  Akio Fujii ,  Hiroo Hoshino ,  Kenichi Matsubara ,  Takemitsu Nagahata ,  Katsutoshi Takahashi ,  Yukihiro Nishiyama

IPC分类号： C07D473/00 ,  C07D501/00 ,  C07H19/02

CPC分类号： C07D473/00 ,  C07D501/00 ,  C07H19/02

摘要： This invention relates to novel oxetanocins represented by the following general formula (I): ##STR1## wherein R represents a group represented by ##STR2## and their pharmacologically acceptable salts which have antiviral activities.

摘要翻译： 本发明涉及由以下通式（I）表示的新型氧杂环丁酮：其中R表示由IMAGE＆lt; IMAGE＆gt;表示的基团及其具有抗病毒活性的药理学上可接受的盐。

公开(公告)号：US5179084A

公开(公告)日：1993-01-12

申请号：US504393

申请日：1990-04-04

申请人： Seiichi Saito ,  Shigeru Hasegawa ,  Masayuki Kitagawa ,  Nobuyoshi Shimada ,  Katsutoshi Takahashi ,  Jun-ichi Seki ,  Hiroo Hoshino ,  Yukihiro Nishiyama ,  Kenichi Matsubara ,  Takemitsu Nagahata

发明人： Seiichi Saito ,  Shigeru Hasegawa ,  Masayuki Kitagawa ,  Nobuyoshi Shimada ,  Katsutoshi Takahashi ,  Jun-ichi Seki ,  Hiroo Hoshino ,  Yukihiro Nishiyama ,  Kenichi Matsubara ,  Takemitsu Nagahata

IPC分类号： C07H19/02

CPC分类号： C07H19/02

摘要： The present invention relates to phosphoric acid esters of oxetanocins having an antiviral activity which are represented by general formula (I): ##STR1## wherein R.sub.1 represents a phosphoric acid ester residue, X represents hydrogen, hydroxy or hydroxymethyl group, and B represents a purine base residue, and pharmacologically acceptable salts thereof.

公开(公告)号：US4247708A

公开(公告)日：1981-01-27

申请号：US006162

申请日：1979-01-24

申请人： Yukihiro Tsutsumi ,  Yutaka Fukuda ,  Kenichi Matsubara

发明人： Yukihiro Tsutsumi ,  Yutaka Fukuda ,  Kenichi Matsubara

IPC分类号： C01G23/00 ,  C01B13/00 ,  C01B13/14 ,  C01B33/18 ,  C01B33/26 ,  C01G23/04 ,  C08G83/00 ,  C08K9/06 ,  C09C3/00 ,  C09C3/12 ,  C07F7/08

CPC分类号： C08G83/001 ,  C08K9/06 ,  C09C3/006 ,  C01P2006/90

摘要： A method for imparting a hydrophobic property to a fine oxide powder by treating it with an organo-polysiloxane in the presence of an alkali catalyst. The method is carried out with at least 1.5% by weight of organo-polysiloxane of molecular weight not exceeding 10,000, at least 0.5% by weight of ammonia or an aliphatic amine of a boiling point lower than 100.degree. C. and at least 1.5% by weight of water are added to a dry weight of the fine oxide powder (a quantity obtained after removal of adsorption water content under reduced pressure not exceeding 5 mmHg in mercury meter at 100.degree. to 110.degree. C.). Of these additives, the ammonia or the aliphatic amine of a boiling point not exceeding 100.degree. C. is added at a temperature not exceeding 60.degree. C. Then, after an aging process for at least 15 minutes, heating is carried out to a temperature between 60.degree. and 150.degree.0 C. for a period of at least 30 minutes. All processes are carried out under normal pressure.

摘要翻译： 通过在碱催化剂的存在下用有机聚硅氧烷处理，赋予氧化物微粉末疏水性的方法。 该方法用至少1.5重量％的分子量不超过10,000的有机聚硅氧烷，至少0.5重量％的氨或沸点低于100℃的脂族胺和至少1.5重量％ 加入到氧化粉末的干重中（在100℃〜110℃下，除去吸附水含量在减压下不超过5mmHg的汞米中得到的量）。 在这些添加剂中，在不超过60℃的温度下加入沸点不超过100℃的氨或脂族胺。然后，在老化过程至少15分钟后，加热至 在60°至150°C之间至少30分钟。 所有过程都在常压下进行。

公开(公告)号：US4927531A

公开(公告)日：1990-05-22

申请号：US315857

申请日：1989-02-27

申请人： Kiyoaki Sakamoto ,  Kenichi Matsubara ,  Yasuhiro Nakahara ,  Masaki Nomura

发明人： Kiyoaki Sakamoto ,  Kenichi Matsubara ,  Yasuhiro Nakahara ,  Masaki Nomura

IPC分类号： B01D15/08 ,  G01N30/52 ,  G01N30/56

CPC分类号： G01N30/56 ,  B01D15/206 ,  G01N2030/522

摘要： To form a gel bed in a cylindrical column for liquid chromatography, a gel bed section is provided between upper and lower liquid-tight partition members, positioned apart from each other at upper and lower positions in the column. Liquid is continuously percolated downward through the gel bed section to form a gel bed in the gel bed section and a liquid pressure is applied onto the upper partition to move the partition member downward following a downward movement of the upper surface of the gel bed.

公开(公告)号：US20060123505A1

公开(公告)日：2006-06-08

申请号：US10449902

申请日：2003-05-29

申请人： Shoshi Kikuchi ,  Naoki Kishimoto ,  Kouji Satoh ,  Toshifumi Nagata ,  Nobuyuki Kawagashira ,  Junshi Yazaki ,  Masahiro Ishikawa ,  Koji Doi ,  Jun Kawai ,  Yoshihide Hayashizaki ,  Yasuhiro Otomo ,  Kenichi Matsubara ,  Kazuo Murakami

发明人： Shoshi Kikuchi ,  Naoki Kishimoto ,  Kouji Satoh ,  Toshifumi Nagata ,  Nobuyuki Kawagashira ,  Junshi Yazaki ,  Masahiro Ishikawa ,  Koji Doi ,  Jun Kawai ,  Yoshihide Hayashizaki ,  Yasuhiro Otomo ,  Kenichi Matsubara ,  Kazuo Murakami

IPC分类号： A01H5/00 ,  G06F19/00 ,  C07H21/04 ,  A01H1/00 ,  C07K14/415 ,  C07K16/18

CPC分类号： C07K14/415

摘要： Full-length cDNAs of plants and their uses are provided. Source plants are preferably monocot plants, more preferably poaceous plants, and most preferably rice. Vectors carrying said cDNAs and transformants containing said cDNAs or said vectors, transgenic plants containing said transformants, polypeptides encoded by said cDNAs are also provided. The full-length cDNA clones play important roles in the annotation of correct gene coding region, determination of exons and introns, comprehensive expression analysis on the transcription level and proteome analysis. Furthermore, full-length cDNA clones are industrially useful in producing plants having different properties from those of the wild type due to the inhibition of expression and functional suppression in plant bodies.

摘要翻译： 提供植物的全长cDNA及其用途。 源植物优选为单子叶植物，更优选为植物，最优选为稻。 还提供了携带所述cDNAs和含有所述cDNA或所述载体的转化体的载体，含有所述转化体的转基因植物，由所述cDNA编码的多肽。 全长cDNA克隆在正确的基因编码区域的注释，外显子和内含子的测定，转录水平的综合表达分析和蛋白质组分析中起重要作用。 此外，全长cDNA克隆在工业上可用于生产与野生型具有不同性质的植物，这是由于植物体中表达和功能抑制的抑制。

公开(公告)号：US5024938A

公开(公告)日：1991-06-18

申请号：US883138

申请日：1986-07-08

申请人： Chikateru Nozaki ,  Atsushi Miyanohara ,  Fukusaburo Hamada ,  Nobuya Ohtomo ,  Kenichi Matsubara

发明人： Chikateru Nozaki ,  Atsushi Miyanohara ,  Fukusaburo Hamada ,  Nobuya Ohtomo ,  Kenichi Matsubara

IPC分类号： C12N15/09 ,  A61K39/29 ,  C07H21/04 ,  C07K14/02 ,  C12N5/10 ,  C12P21/00 ,  C12P21/02

CPC分类号： C07K14/005 ,  C12N2730/10122

摘要： A recombinant DNA comprising 3 fragments of Hepatitis B virus DNA recombined with a vector consisting essentially of 0.31 Kb replication orgin of SV40 DNA inserted into EcoRI cleavage site of Escherichia coli plasmid which is deficient in the 1.426-2.521 Kb region of inhibiting replication in mammalian cells, wherein each HBV DNA fragment is a 3.2 Kb BamHI fragment consisting of 1.9 Kb HBc gene and 1.3 Kb HBs gene, and said HBV DNA fragments are arranged in a head-to-tail tandem relationship wherein the HBc gene positions at the head and the HBs gene positions at the tail, mammalian cells transformed with the recombinant DNA, and a method of production of Hepatitis B virus proteins, i.e. HBsAg and/or HBeAg. These HBV proteins have the same immunological properties as those of the natural HBV proteins originated from human blood plasma and can be used for the preparation of HBV vaccine and diagnostic reagents.

摘要翻译： 将包含3个乙型肝炎病毒DNA片段的重组DNA与基本上由插入到在哺乳动物细胞中抑制复制的1.426-2.521Kb区域的缺失的大肠杆菌质粒的EcoRI切割位点的SV40DNA的0.31Kb复制形式的载体重组 ，其中每个HBV DNA片段是由1.9Kb HBc基因和1.3Kb HBs基因组成的3.2Kb BamHI片段，并且所述HBV DNA片段以头对尾串联关系排列，其中HBc基因位于头部和 在尾部的HBs基因位置，用重组DNA转化的哺乳动物细胞，以及生产乙型肝炎病毒蛋白即HBsAg和/或HBeAg的方法。 这些HBV蛋白质具有与源自人血浆的天然HBV蛋白质相同的免疫学特性，可用于制备HBV疫苗和诊断试剂。

公开(公告)号：US4778761A

公开(公告)日：1988-10-18

申请号：US4424

申请日：1987-01-20

申请人： Atsushi Miyanohara ,  Chikateru Nozaki ,  Fukusaburo Hamada ,  Akio Toh-e ,  Nobuya Ohtomo ,  Kenichi Matsubara

发明人： Atsushi Miyanohara ,  Chikateru Nozaki ,  Fukusaburo Hamada ,  Akio Toh-e ,  Nobuya Ohtomo ,  Kenichi Matsubara

IPC分类号： C12N15/09 ,  A61K39/29 ,  C07H21/04 ,  C07K14/02 ,  C12N15/00 ,  C12N15/62 ,  C12N15/80 ,  C12N15/81 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/865 ,  C12N1/00

CPC分类号： C07K14/005 ,  C12N15/62 ,  C12N15/80 ,  C12N15/81 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2730/10122

摘要： A recombinant plasmid inserted with Hepatitis B virus gene, which comprises a plasmid vector containing a yeast gene and an E. coli gene and carrying the expression control region of the repressible acid phosphatase gene of yeast and a Hepatitis B virus gene recombined thereto under control of the phosphatase promoter, a transformed yeast which is prepared by transforming a yeast with the recombinant plasmid, and a method of the production of Hepatitis B virus surface antigen in a large scale by culturing the transformed yeast in a medium. The Hepatitis B virus surface antigen prepared by the present invention has the same immunological properties as those of the natural antigen from human blood plasma and is useful for the preparation of Hepatitis B virus vaccine and diagnostic reagents.

摘要翻译： 插入乙型肝炎病毒基因的重组质粒，其包含含有酵母基因和大肠杆菌基因的质粒载体，并携带酵母的可压制酸性磷酸酶基因的表达控制区和重组的乙型肝炎病毒基因的控制区 磷酸酶启动子，通过用重组质粒转化酵母制备的转化酵母以及通过在培养基中培养转化的酵母大规模生产乙型肝炎病毒表面抗原的方法。 本发明制备的乙型肝炎病毒表面抗原具有与人血浆天然抗原相同的免疫学特性，可用于制备乙型肝炎病毒疫苗和诊断试剂。

公开(公告)号：US5707862A

公开(公告)日：1998-01-13

申请号：US622344

申请日：1990-12-05

申请人： Atsushi Miyanohara ,  Akio Toh-e ,  Kenichi Matsubara

发明人： Atsushi Miyanohara ,  Akio Toh-e ,  Kenichi Matsubara

IPC分类号： C12N15/09 ,  A61K39/29 ,  C07H21/04 ,  C07K14/02 ,  C12N1/19 ,  C12N15/00 ,  C12N15/62 ,  C12N15/81 ,  C12P19/34 ,  C12P21/00 ,  C12R1/19 ,  C12R1/865

CPC分类号： C07K14/005 ,  C12N15/62 ,  C12N15/81 ,  C07K2319/00 ,  C12N2730/10122

摘要： A shuttle vector containing an yeast gene and an E. coli gene and carrying the expression control region of acid phosphatase gene of yeast, which can be recombined with various genes under control of the phosphatase promoter, to give various recombinant plasmids. The shuttle vector is useful in genetic engineering industries.

摘要翻译： 含有酵母基因和大肠杆菌基因的穿梭载体，并携带可与磷酸酶启动子控制下的各种基因重组的酵母酸性磷酸酶基因的表达控制区，得到各种重组质粒。 穿梭载体在基因工程行业很有用。