发明授权
- 专利标题: Animals transgenic for a tetracycline-regulated transcriptional inhibitor
- 专利标题(中): 四环素调节转录抑制剂转基因动物
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申请号: US486814申请日: 1995-06-07
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公开(公告)号: US5866755A公开(公告)日: 1999-02-02
- 发明人: Hermann Bujard , Manfred Gossen
- 申请人: Hermann Bujard , Manfred Gossen
- 申请人地址: DEX DEX
- 专利权人: BASF Aktiengellschaft,Knoll Aktiengellschaft
- 当前专利权人: BASF Aktiengellschaft,Knoll Aktiengellschaft
- 当前专利权人地址: DEX DEX
- 主分类号: A01K67/027
- IPC分类号: A01K67/027 ; A61K48/00 ; C07K14/00 ; C07K14/035 ; C07K14/195 ; C07K14/245 ; C07K19/00 ; C12N1/15 ; C12N1/19 ; C12N5/10 ; C12N15/09 ; C12N15/11 ; C12N15/12 ; C12N15/62 ; C12N15/63 ; C12N15/67 ; C12N15/85 ; C12N5/00 ; C12N15/00
摘要:
Transgenic animals carrying a transgene comprising a nucleic acid molecule encoding protein useful for regulating the expression of genes in eukaryotic cells and organisms in a highly controlled manner are disclosed. In the regulatory system of the invention, transcription of a tet operator-linked nucleotide sequence is inhibited by a transcriptional inhibitor fusion protein composed of two polypeptides, a first polypeptide which binds to tet operator sequences and a second polypeptide which directly or indirectly inhibits transcription in eukaryotic cells. In various embodiment, the first polypeptide binds to tet operator sequences either: (i) in the absence but not the presence of tetracycline (or an analogue thereof) or (ii) in the presence but not the absence of tetracycline (or an analogue thereof). In a preferred embodiment, the transgene encoding the transcriptional inhibitor fusion protein is integrated at a predetermined location within the chromosome of the transgenic animal.