发明授权
- 专利标题： Method of preparing a variant of a lipolytic enzyme
- 专利标题（中）： 制备脂肪分解酶变体的方法
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申请号： US701339申请日： 1996-08-22
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公开(公告)号： US5976855A公开(公告)日： 1999-11-02
- 发明人: Allan Svendsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
- 申请人： Allan Svendsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
- 申请人地址： DKX Bagsv.ae butted.rt
- 专利权人： Novo Nordisk A/S
- 当前专利权人： Novo Nordisk A/S
- 当前专利权人地址： DKX Bagsv.ae butted.rt
- 优先权： DKX0217/94 19940222
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C11D3/386 ; C11D7/42 ; C12N1/19 ; C12N1/21 ; C12N9/20 ; C12N15/55 ; C12R1/69 ; C07H21/04 ; C12N1/00 ; C12N1/20
摘要：
The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
公开/授权文献
- US5128665A Selective call signalling system 公开/授权日：1992-07-07
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