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公开(公告)号:US5976855A
公开(公告)日:1999-11-02
申请号:US701339
申请日:1996-08-22
IPC分类号: C12N15/09 , C11D3/386 , C11D7/42 , C12N1/19 , C12N1/21 , C12N9/20 , C12N15/55 , C12R1/69 , C07H21/04 , C12N1/00 , C12N1/20
CPC分类号: C12N9/20 , C11D3/38627 , C11D3/38663 , Y10S435/832 , Y10S435/849 , Y10S435/874 , Y10S435/886 , Y10S435/911 , Y10S435/913
摘要: The present invention relates to a method of preparing a variant of a parent lipolytic enzyme, comprising (a) subjecting a DNA sequence encoding the parent lipolytic enzyme to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and (c) screening for host cells expressing a mutated lipolytic enzyme which has a decreased dependance to calcium and/or an improved tolerance towards a detergent or a detergent component as compared to the parent lipolytic enzyme.
摘要翻译: 本发明涉及一种制备母体脂肪分解酶的变体的方法,其包括(a)使编码亲本脂肪分解酶的DNA序列进行随机诱变,(b)将步骤(a)中获得的突变的DNA序列表达于 宿主细胞,和(c)与母体脂肪分解酶相比,筛选表达与钙相关性降低和/或改善的对洗涤剂或洗涤剂组分的耐受性的突变的脂肪分解酶的宿主细胞。
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公开(公告)号:US5866526A
公开(公告)日:1999-02-02
申请号:US619753
申请日:1996-05-02
申请人: Arne Agerlin Olsen , Allan Svendsen , Kim Borch , Henrik Lund , Marianne Thellersen , Peter Rosholm , Niels Munk
发明人: Arne Agerlin Olsen , Allan Svendsen , Kim Borch , Henrik Lund , Marianne Thellersen , Peter Rosholm , Niels Munk
IPC分类号: D06L1/00 , C11D3/386 , C12N9/20 , C12N9/24 , C12N9/26 , C12N9/42 , C12N9/96 , C12S3/04 , C12S3/06 , C12S11/00 , D06M15/15 , D21C9/10
CPC分类号: C12N9/2411 , C11D3/386 , C11D3/38627 , C11D3/38636 , C11D3/38645 , C11D3/38654 , C12N9/20 , C12N9/2408 , C12N9/2482 , C12N9/96 , C12Y302/01008
摘要: An enzyme preparation comprising a modified enzyme selected from the group consisting of an amylase, lipase, oxidorcductase, pectinace or hemicellulase, the modified enzyme having an improved performance due to an alkaline pI and/or increased surface activity obtained by chemical modification or amino acid substitution, is useful e.g., in detergents, in baking flour, in animal feed, in the manufacture of cellulosic fabrics and for the treatment of lignocellulosic fibers.
摘要翻译: PCT No.PCT / DK94 / 00368 Sec。 371日期:1996年5月2日 102(e)日期1996年5月2日PCT 1994年10月4日PCT PCT。 公开号WO95 / 09909 日期1995年4月13日含有选自淀粉酶,脂肪酶,氧化还原酶,果胶或半纤维素酶的修饰酶的酶制剂,由于碱性pI具有改善的性能和/或由化学物质获得的增加的表面活性的改性酶 改性或氨基酸取代,例如在洗涤剂,烘焙粉,动物饲料,纤维素织物的制造和木质纤维素纤维的处理中都是有用的。
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公开(公告)号:US5753460A
公开(公告)日:1998-05-19
申请号:US720899
申请日:1996-10-10
申请人: Henrik Bisgard-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
发明人: Henrik Bisgard-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
IPC分类号: C12N15/09 , C11D3/386 , C12N9/28 , C12N15/56 , C12R1/125 , D06L1/14 , C12P21/06 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号: C12N9/2417 , C11D3/386 , D06L1/14
摘要: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.
摘要翻译: 与母体酶相比,具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体,其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中,条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改,母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。
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公开(公告)号:US5869438A
公开(公告)日:1999-02-09
申请号:US479275
申请日:1995-06-07
申请人: Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Jens Sigurd Okkels , Marianne Thellersen
发明人: Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Jens Sigurd Okkels , Marianne Thellersen
CPC分类号: C12N9/20 , C11D3/38627 , C11D3/38663 , C11D3/38672 , C07K2319/04
摘要: The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.
摘要翻译: 本发明涉及表现出改善的性质的脂肪酶变体,包含所述脂肪酶变体的洗涤剂组合物,编码所述脂肪酶变体的DNA构建体,以及制备所述脂肪酶变体的方法。
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公开(公告)号:US5830837A
公开(公告)日:1998-11-03
申请号:US343804
申请日:1994-11-22
申请人: Henrik Bisg.ang.rd-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
发明人: Henrik Bisg.ang.rd-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
CPC分类号: C11D3/386
摘要: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.
摘要翻译: 与母体酶相比,具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体,其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中,条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改,母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。
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公开(公告)号:US5801043A
公开(公告)日:1998-09-01
申请号:US459610
申请日:1995-06-02
申请人: Henrik Bisg.ang.rd-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
发明人: Henrik Bisg.ang.rd-Frantzen , Torben Vedel Borchert , Allan Svendsen , Marianne Thellersen , Pia Van der Zee
CPC分类号: C11D3/386
摘要: A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.
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公开(公告)号:US07157262B2
公开(公告)日:2007-01-02
申请号:US10232544
申请日:2002-08-30
申请人: Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人: Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
CPC分类号: C11D3/38627 , C12N9/20
摘要: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzyme capable of removing a substantial amount of fatty matter during a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译: 本发明涉及具有脂肪分解活性的修饰酶,能够在一次循环洗涤期间除去大量脂肪物质的脂肪分解酶,编码所述酶的DNA序列,包含所述DNA序列的载体,携带所述DNA的宿主细胞 序列或所述载体,以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号:US06495357B1
公开(公告)日:2002-12-17
申请号:US09007288
申请日:1998-01-14
申请人: Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
发明人: Claus Crone Fuglsang , Jens Sigurd Okkels , Dorte Aaby Petersen , Shamkant Anant Patkar , Marianne Thellersen , Allan Svendsen , Kim Borch , John C. Royer , Titus Kretzschmar , Torben Halkier , Jesper Vind , Steen Troels Jorgensen
IPC分类号: C12N920
CPC分类号: C11D3/38627 , C12N9/20
摘要: The present invention relates to a modified enzyme with lipolytic activity, a lipolytic enzime capable of removing a substantial amount of fatty matter a one cycle wash, a DNA sequence encoding said enzymes, a vector comprising said DNA sequence, a host cell harbouring said DNA sequence or said vector, and a process for producing said enzymes with lipolytic activity.
摘要翻译: 本发明涉及一种具有脂肪分解活性的修饰酶,能够一周期洗涤大量脂肪物质的脂肪分解酶,编码所述酶的DNA序列,包含所述DNA序列的载体,携带所述DNA序列的宿主细胞 或所述载体,以及用于产生具有脂肪分解活性的所述酶的方法。
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公开(公告)号:US5892013A
公开(公告)日:1999-04-06
申请号:US488271
申请日:1995-09-05
申请人: Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
发明人: Allan Svendsen , Shamkant Anant Patkar , Erik Gormsen , Ib Groth Clausen , Jens Sigurd Okkels , Marianne Thellersen
CPC分类号: C11D3/38627 , C11D3/38663 , C11D3/38672 , C12N9/20 , C07K2319/04
摘要: The present invention relates to lipase variants which exhibit improved properties, detergent compositions comprising said lipase variants, DNA constructs coding for said lipase variants, and methods of making said lipase variants.
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