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发明授权
US5994066A Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories 失效
物种特异性和通用DNA探针和扩增引物，用于快速检测和鉴定临床标本中常见的细菌病原体和相关抗生素抗性基因，用于微生物实验室的常规诊断

Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
摘要：
The present invention relates to a method for universal detection of bacteria in biological samples and for specific detection of Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus saprophyticus, Streptococcus pneumoniae, Moraxella catarrhalis and Haemophilus influenzae in urine or any other biological samples, said method comprising denaturation of bacterial DNA to single stranded form and either fixing it on a support or leaving it in solution, contacting said single stranded genetic material with a labeled probe selected from the group consisting of i) fragments of chromosomal DNA of the above-mentioned bacteria and ii) synthetic oligonucleotides whose sequences are derived either from the said fragments of chromosomal DNAs or from sequences available in data banks, all (i and ii) probes being capable to hybridize specifically to their chromosomal DNA or, in case of universal probes, to any bacterial chromosomal DNA.
摘要（中）：

本发明涉及生物样品中细菌的普遍检测方法，特异性检测大肠杆菌，肺炎克雷伯杆菌，粪肠球菌，铜绿假单胞菌，奇异变形杆菌，腐生葡萄球菌，肺炎链球菌，卡他性莫拉氏菌和流感嗜血杆菌在尿液或任何 所述方法包括将细菌DNA变性为单链形式并将其固定在载体上或将其留在溶液中，使所述单链遗传物质与选自以下的标记探针接触：i）染色体DNA片段 的上述细菌，和ii）其序列衍生自所述染色体DNA片段或数据库中可获得的序列的合成寡核苷酸，所有（i和ii）探针能够特异性与其染色体DNA杂交，或 通用探针的情况，任何细菌染色体 脱氧核糖核酸。

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