发明授权
US06902920B2 Method for producing an active heterodimeric AMV-RT in prokaryotic cells
有权
用于在原核细胞中产生活性异二聚体AMV-RT的方法
- 专利标题: Method for producing an active heterodimeric AMV-RT in prokaryotic cells
- 专利标题(中): 用于在原核细胞中产生活性异二聚体AMV-RT的方法
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申请号: US09960428申请日: 2001-09-21
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公开(公告)号: US06902920B2公开(公告)日: 2005-06-07
- 发明人: Harald Sobek , Rainer Mueller , Manfred Schmidt , Bruno Frey , Bernhard Suppmann , Rainer Schmuck , Johann-Peter Thalhofer , Peter Pallua , Markus Pajatsch
- 申请人: Harald Sobek , Rainer Mueller , Manfred Schmidt , Bruno Frey , Bernhard Suppmann , Rainer Schmuck , Johann-Peter Thalhofer , Peter Pallua , Markus Pajatsch
- 申请人地址: US IN Indianaoplis
- 专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人: Roche Diagnostics Operations, Inc.
- 当前专利权人地址: US IN Indianaoplis
- 代理机构: Roche Diagnostics Operations, Inc.
- 代理商 Marilyn Amick
- 优先权: DE10046960 20000922
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12N1/21 ; C12N9/12 ; C12N15/54
摘要:
The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.
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