Invention Grant
US06942994B2 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
失效
用于改变植物中酶和乙酰CoA水平的材料和方法
- Patent Title: Materials and methods for the alteration of enzyme and acetyl CoA levels in plants
- Patent Title (中): 用于改变植物中酶和乙酰CoA水平的材料和方法
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Application No.: US10293865Application Date: 2002-11-13
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Publication No.: US06942994B2Publication Date: 2005-09-13
- Inventor: Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
- Applicant: Basil J. Nikolau , Eve S. Wurtele , David J. Oliver , Robert Behal , Patrick S. Schnable , Jinshan Ke , Jerry L. Johnson , Carolyn C. Allred , Beth Fatland , Isabelle Lutziger , Tsui-Jung Wen
- Applicant Address: US IA Ames
- Assignee: Iowa State University Research Foundation, Inc.
- Current Assignee: Iowa State University Research Foundation, Inc.
- Current Assignee Address: US IA Ames
- Agency: Gardner Carton & Douglas LLP
- Main IPC: A01H1/00
- IPC: A01H1/00 ; C12N5/04 ; C12N9/00 ; C12N9/02 ; C12N9/10 ; C12N9/16 ; C12N9/88 ; C12N15/113 ; C12N15/82 ; C12N15/09 ; A01H5/00 ; C12N5/10

Abstract:
The present invention provides nucleic acid and amino acid sequences of acetyl CoA synthetase (ACS), plastidic pyruvate dehydrogenase (pPDH), ATP citrate lyase (ACL), Arabidopsis pyruvate decarboxylase (PDC), and Arabidopsis aldehyde dehydrogenase (ALDH), specifically ALDH-2 and ALDH-4. The present invention also provides a recombinant vector comprising a nucleic acid sequence encoding one of the aforementioned enzymes, an antisense sequence thereto or a ribozyme therefor, a cell transformed with such a vector, antibodies to the enzymes, a plant cell, a plant tissue, a plant organ or a plant in which the level of an enzyme has been altered, and a method of producing such a plant cell, plant tissue, plant organ or plant. Desirably, alteration of the level of enzyme results in an alteration of the level of acetyl CoA in the plant cell, plant tissue, plant organ or plant. In addition, the present invention provides a recombinant vector comprising an antisense sequence of a nucleic acid sequence encoding pyruvate decarboxylase (PDC), the E1α subunit of pPDH, the E1β subunit of pPDH, the E2 subunit of pPDH, mitochondrial pyruvate dehydrogenase (mtPDH) or aldehyde dehydrogenase (ALDH) or a ribozyme that can cleave an RNA molecule encoding PDC, E1α pPDH, E1β pPDH, E2 pPDH, mtPDH or ALDH.
Public/Granted literature
- US20030106090A1 Materials and methods for the alteration of enzyme and acetyl CoA levels in plants Public/Granted day:2003-06-05
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