发明授权
- 专利标题: Enzymes for the production of 2-keto-L-gulonic acid
- 专利标题(中): 用于生产2-酮-L-古洛糖酸的酶
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申请号: US09332886申请日: 1999-06-15
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公开(公告)号: US07256027B1公开(公告)日: 2007-08-14
- 发明人: Robert A. Lazarus , Mark Hurle , Stephen Anderson , David B. Powers
- 申请人: Robert A. Lazarus , Mark Hurle , Stephen Anderson , David B. Powers
- 申请人地址: US NJ New Brunswick
- 专利权人: Rutgers, the State University of New Jersey
- 当前专利权人: Rutgers, the State University of New Jersey
- 当前专利权人地址: US NJ New Brunswick
- 代理机构: Licata & Tyrrell P.C.
- 主分类号: C12N9/02
- IPC分类号: C12N9/02 ; C12N15/00 ; C12N1/20 ; C12Q1/20 ; C12Q1/00 ; C12Q1/26 ; C12Q21/04 ; C07H21/04
摘要:
Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.
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