发明授权
- 专利标题： Nitrile hydratase variant
- 专利标题（中）： 腈水合酶变体
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申请号： US10539560申请日： 2003-12-15
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公开(公告)号： US07595184B2公开(公告)日： 2009-09-29
- 发明人: Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
- 申请人： Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
- 申请人地址： JP Minato-Ku, Tokyo
- 专利权人： Mitsui Chemicals, Inc.
- 当前专利权人： Mitsui Chemicals, Inc.
- 当前专利权人地址： JP Minato-Ku, Tokyo
- 代理机构： Buchanan Ingersoll & Rooney PC
- 优先权： JP2002-368360 20021219; JP2003-379280 20031110
- 国际申请： PCT/JP03/16014 WO 20031215
- 国际公布： WO2004/056990 WO 20040708
- 主分类号： C12N9/88
- IPC分类号： C12N9/88 ; C12N5/10 ; C12N1/21 ; C07K14/00 ; C12P21/00 ; C12N15/00 ; C12Q1/527 ; C07H21/00
摘要：
The amino acid sequence of a mutant which is obtained by introducing a novel mutation into a Pseudonocardia thermophila JCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.
公开/授权文献
- US20070009985A1 Novel nitrile hydratase 公开/授权日：2007-01-11
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