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公开(公告)号：US07510861B2
公开(公告)日：2009-03-31
申请号：US11523691
申请日：2006-09-20
申请人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
发明人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
CPC分类号： C12N9/88 , C12P7/58 , C12Y402/01039
摘要： A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
摘要翻译： 本发明提供了一种衍生自木糖无色杆菌的新型葡萄糖酸脱水酶和编码葡萄糖酸脱水酶的基因。 通过使葡糖酸脱水酶或含有该基因的转化细胞与醛糖酸反应，可以有效地制备相应的2-酮-3-脱氧醛酸。
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公开(公告)号：US07595184B2
公开(公告)日：2009-09-29
申请号：US10539560
申请日：2003-12-15
申请人： Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
发明人： Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
CPC分类号： C12Y402/01084 , C12N9/88
摘要： The amino acid sequence of a mutant which is obtained by introducing a novel mutation into a Pseudonocardia thermophila JCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.
摘要翻译： 提供了通过将新突变引入由两种异质亚基组成的嗜热假诺卡氏菌JCM3095衍生的腈水合酶获得的突变体的氨基酸序列和该基因的碱基序列。 通过在腈水合酶的立体结构/氨基酸序列中指定待修饰的区域，并将替代，插入，缺失等的改变应用于氨基酸序列中相应的氨基酸序列中的氨基酸来修饰腈水合酶 到形成该区域的氨基酸残基。 还提供了修饰具有腈水合酶活性的酶的方法。
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公开(公告)号：US07125704B2
公开(公告)日：2006-10-24
申请号：US10885874
申请日：2004-07-08
申请人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
发明人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
CPC分类号： C12N9/88 , C12P7/58 , C12Y402/01039
摘要： A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
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4.发明授权DNA encoding novel D-aminoacylase and process for producing D-amino acid by using the same 失效编码新型D-氨基酸酰基酶的DNA和使用它们生产D-氨基酸的方法公开(公告)号：US06869788B2
公开(公告)日：2005-03-22
申请号：US10240422
申请日：2002-02-01
IPC分类号： C12N1/21 , C12N9/80 , C12P13/04 , C12P41/00 , C12N9/78 , C07H21/04 , C07K17/00 , C12P13/22 , C12Q1/34
CPC分类号： C12P41/007 , C12N9/80 , C12P13/04 , C12Y305/01014
摘要： This invention provides a novel D-aminoacylase obtained by cloning a DNA encoding the novel D-aminoacylase from Methylobacterium mesophilicum MT 10894, etc. and which shows sufficiently high activity at an industrially useful substrate concentration to allow a D-amino acid to be efficiently produced from an N-acyl-DL-amino acid; a DNA encoding the D-aminoacylase; a process for producing a D-amino acid from the corresponding N-acylamino acid using a transformant containing the DNA.
摘要翻译： 本发明提供了一种通过克隆编码来自嗜甲基嗜甲基菌MT10894等的新型D-氨基酰化酶的DNA获得的新型D-氨基酰化酶，并且在工业上有用的底物浓度下显示出足够高的活性以允许有效地产生D-氨基酸 来自N-酰基-DL-氨基酸; 编码D-氨基酰化酶的DNA; 使用含有DNA的转化体从相应的N-酰基氨基酸生产D-氨基酸的方法。
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公开(公告)号：US20050054042A1
公开(公告)日：2005-03-10
申请号：US10885874
申请日：2004-07-08
申请人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
发明人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
CPC分类号： C12N9/88 , C12P7/58 , C12Y402/01039
摘要： A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
摘要翻译： 本发明提供了一种衍生自木糖无色杆菌的新型葡萄糖酸脱水酶和编码葡萄糖酸脱水酶的基因。 通过使葡糖酸脱水酶或含有该基因的转化细胞与醛糖酸反应，可以有效地制备相应的2-酮-3-脱氧醛酸。
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公开(公告)号：US5910432A
公开(公告)日：1999-06-08
申请号：US990818
申请日：1997-12-15
申请人： Kiyoshi Ito , Toshifumi Yamaki , Teruo Arii , Miyuki Tsuruoka , Takeshi Nakamura
发明人： Kiyoshi Ito , Toshifumi Yamaki , Teruo Arii , Miyuki Tsuruoka , Takeshi Nakamura
CPC分类号： C12N9/78 , Y10S435/822
摘要： The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.
摘要翻译： 本发明提供了嗜热假诺卡氏菌腈水合酶的氨基酸序列和碱基序列，进一步提供了改变其氨基酸序列和碱基序列而基本上不改变所述腈水合酶的功能的方法，以及具有碱基序列的腈水合酶 和基于所述方法改变的氨基酸序列，并且还提供具有所述腈水合酶基因的重组质粒，含有所述重组质粒的转化体，使用所述转化体产生所述酶的方法，和 使用所述转化体从腈化合物制备相应的酰胺化合物。
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公开(公告)号：US20070015265A1
公开(公告)日：2007-01-18
申请号：US11523691
申请日：2006-09-20
申请人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
发明人： Hitoki Miyake , Toshifumi Yamaki , Toshihiro Oikawa , Takeshi Nakamura , Hiroki Ishibashi , Yasushi Fukuiri , Atsushi Sakuma , Hironori Komatsu , Tomoyuki Ando , Kazuhiko Togashi , Hideki Umetani
CPC分类号： C12N9/88 , C12P7/58 , C12Y402/01039
摘要： A novel gluconate dehydratase derived from Achromobacter xylosoxidans and a gene encoding the gluconate dehydratase are provided. By reacting the gluconate dehydratase or a transformed cell containing the gene with an aldonic acid, the corresponding 2-keto-3-deoxyaldonic acid can be efficiently produced.
摘要翻译： 本发明提供了一种衍生自木糖无色杆菌的新型葡萄糖酸脱水酶和编码葡萄糖酸脱水酶的基因。 通过使葡糖酸脱水酶或含有该基因的转化细胞与醛糖酸反应，可以有效地制备相应的2-酮-3-脱氧醛酸。
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公开(公告)号：US20070009985A1
公开(公告)日：2007-01-11
申请号：US10539560
申请日：2003-12-15
申请人： Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
发明人： Toshifumi Yamaki , Shinichi Banba , Kaori Matoishi , Kiyoshi Ito , Hideki Kobayashi , Eishi Tanaka , Toshihiro Oikawa
CPC分类号： C12Y402/01084 , C12N9/88
摘要： The amino acid sequence of a mutant which is obtained by introducing a novel mutation into a Pseudonocardia thermophila JCM3095-derived nitrile hydratase consisting of two types of heterogeneous subunits, and the base sequence of the gene are provided. The nitrile hydratase is modified by specifying the region to be modified in the stereostructure/amino acid sequence of the nitrile hydratase, and applying alteration such as substitution, insertion, deletion or the like, to the amino acids in the amino acid sequence which are corresponding to the amino acid residues forming the region. Also provided is a method for modifying an enzyme having a nitrile hydratase activity.
摘要翻译： 提供了通过将新突变引入由两种异质亚基组成的嗜热假诺卡氏菌JCM3095衍生的腈水合酶获得的突变体的氨基酸序列和该基因的碱基序列。 通过在腈水合酶的立体结构/氨基酸序列中指定待修饰的区域，并将替代，插入，缺失等的改变应用于氨基酸序列中相应的氨基酸序列中的氨基酸来修饰腈水合酶 到形成该区域的氨基酸残基。 还提供了修饰具有腈水合酶活性的酶的方法。
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公开(公告)号：US5807730A
公开(公告)日：1998-09-15
申请号：US800751
申请日：1997-02-14
申请人： Kiyoshi Ito , Toshifumi Yamaki , Teruo Arii , Miyuki Tsuruoka , Takeshi Nakamura
发明人： Kiyoshi Ito , Toshifumi Yamaki , Teruo Arii , Miyuki Tsuruoka , Takeshi Nakamura
CPC分类号： C12N9/78 , Y10S435/822
摘要： The present invention provides the amino acid sequence and base sequence of a Pseudonocardia thermophila-derived nitrile hydratase, provides further a method for changing its amino acid sequence and base sequence without substantially changing the functions of said nitrile hydratase, and nitrile hydratases having a base sequence and an amino acid sequence as changed on the basis of said method, and provides furthermore a recombinant plasmid having the gene of said nitrile hydratase, a transformant containing said recombinant plasmid, a method of using said transformant for producing said enzyme, and a method of using said transformant for producing the corresponding amide compound from a nitrile compound.
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