发明授权
- 专利标题: shRNA library
- 专利标题(中): shRNA文库
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申请号: US12221325申请日: 2008-08-01
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公开(公告)号: US07863222B2公开(公告)日: 2011-01-04
- 发明人: Donald G. Payan , Mary Shen , Simon Yu , Todd M. Kinsella , Yasumichi Hitoshi
- 申请人: Donald G. Payan , Mary Shen , Simon Yu , Todd M. Kinsella , Yasumichi Hitoshi
- 申请人地址: US CA South San Francisco
- 专利权人: Rigel Pharmaceuticals, Inc.
- 当前专利权人: Rigel Pharmaceuticals, Inc.
- 当前专利权人地址: US CA South San Francisco
- 代理机构: Bozicevic, Field & Francis LLP
- 代理商 James S. Keddie; Travis Young
- 主分类号: C40B50/06
- IPC分类号: C40B50/06 ; C40B40/08 ; C40B30/06 ; C40B40/02 ; C12P19/34 ; A61K49/12
摘要:
As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.
公开/授权文献
- US20100029505A1 shRNA library 公开/授权日:2010-02-04
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