Invention Grant
- Patent Title: shRNA library
- Patent Title (中): shRNA文库
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Application No.: US12221325Application Date: 2008-08-01
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Publication No.: US07863222B2Publication Date: 2011-01-04
- Inventor: Donald G. Payan , Mary Shen , Simon Yu , Todd M. Kinsella , Yasumichi Hitoshi
- Applicant: Donald G. Payan , Mary Shen , Simon Yu , Todd M. Kinsella , Yasumichi Hitoshi
- Applicant Address: US CA South San Francisco
- Assignee: Rigel Pharmaceuticals, Inc.
- Current Assignee: Rigel Pharmaceuticals, Inc.
- Current Assignee Address: US CA South San Francisco
- Agency: Bozicevic, Field & Francis LLP
- Agent James S. Keddie; Travis Young
- Main IPC: C40B50/06
- IPC: C40B50/06 ; C40B40/08 ; C40B30/06 ; C40B40/02 ; C12P19/34 ; A61K49/12

Abstract:
As noted above, certain aspects of this disclosure relate to a library of nucleic acid vectors, as well as a method for making the same. In certain embodiments, the library of nucleic acid vectors comprises: a plurality of nucleic acid molecules of the following formula: S1—R—S2 wherein, in each nucleic acid of the plurality: S1 and S2 are each at least 15 nucleotides in length; S1 and S2 are complementary to each other along their entire length; either S1 or S2 is complementary along its entire length to a sequence in eukaryotic mRNA; and R is a six base recognition site for a restriction endonuclease; and wherein S1 and S2 vary in nucleotide sequence between different members of the plurality. A method for amplifying a circular nucleic acid is also provided.
Public/Granted literature
- US20100029505A1 shRNA library Public/Granted day:2010-02-04
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