发明授权
US08143023B2 Method for methanol independent induction from methanol inducible promoters in Pichia
失效
甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法
- 专利标题: Method for methanol independent induction from methanol inducible promoters in Pichia
- 专利标题(中): 甲醇独立诱导甲基诱导启动子在毕赤酵母中的方法
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申请号: US12521856申请日: 2008-01-25
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公开(公告)号: US08143023B2公开(公告)日: 2012-03-27
- 发明人: Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人: Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人地址: DK Bagsvaerd
- 专利权人: Novozymes A/S
- 当前专利权人: Novozymes A/S
- 当前专利权人地址: DK Bagsvaerd
- 代理商 Michael W. Krenicky
- 优先权: DK200700122 20070126
- 国际申请: PCT/EP2008/050870 WO 20080125
- 国际公布: WO2008/090211 WO 20080731
- 主分类号: C12P21/06
- IPC分类号: C12P21/06
摘要:
A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
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