发明授权
- 专利标题： Method for methanol independent induction from methanol inducible promoters in Pichia
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申请号： US13396925申请日： 2012-02-15
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公开(公告)号： US08236528B2公开(公告)日： 2012-08-07
- 发明人: Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人： Shinobu Takagi , Noriko Tsutsumi , Yuji Terui , XiangYu Kong
- 申请人地址： DK Bagsvaerd
- 专利权人： Novozymes A/S
- 当前专利权人： Novozymes A/S
- 当前专利权人地址： DK Bagsvaerd
- 代理商 Michael W. Krenicky
- 优先权： DK200700122 20070126
- 主分类号： C12P21/06
- IPC分类号： C12P21/06
摘要：
A method for producing a polypeptide in a methylotrophic yeast host cell is described, where expression of the polypeptide is controlled by a methanol inducible promoter, including: i) expression of a positive regulator from a non-native promoter, the positive regulator activating transcription from the methanol inducible promoter, and ii) no addition of methanol.
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