发明授权
US08603822B2 Method for designing heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase and heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase
失效
设计耐热性酪氨酸依赖性短链脱氢酶/还原酶和耐热性酪氨酸依赖性短链脱氢酶/还原酶的方法
- 专利标题： Method for designing heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase and heat-resistant tyrosine-dependent short-chain dehydrogenase/reductase
- 专利标题（中）： 设计耐热性酪氨酸依赖性短链脱氢酶/还原酶和耐热性酪氨酸依赖性短链脱氢酶/还原酶的方法
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申请号： US13013239申请日： 2011-01-25
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公开(公告)号： US08603822B2公开(公告)日： 2013-12-10
- 发明人: Daisuke Yamaguchi , Seiji Yamada , Yoshio Goto , Yuichi Tokita
- 申请人： Daisuke Yamaguchi , Seiji Yamada , Yoshio Goto , Yuichi Tokita
- 申请人地址： JP Tokyo
- 专利权人： Sony Corporation
- 当前专利权人： Sony Corporation
- 当前专利权人地址： JP Tokyo
- 代理机构： K&L Gates LLP
- 优先权： JP2010-016675 20100128
- 主分类号： C12N15/00
- IPC分类号： C12N15/00 ; C12N9/04 ; C12N1/20 ; C12P21/06 ; C12P21/04 ; C12Q1/68 ; C07H21/04
摘要：
A method for designing a heat-resistant mutant enzyme, the method including the step of reducing a distance between the α4 helix and the α6 helix in a protein three-dimensional structure to become smaller than that of a wild type enzyme through deletion, replacement, addition, or insertion of one or several amino acids in the amino acid sequence of the wild type enzyme with respect to tyrosine-dependent short-chain dehydrogenase/reductase.
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