发明授权
- 专利标题: DNA polymerases with increased 3′-mismatch discrimination
- 专利标题(中): 具有增加的3'-不匹配鉴别的DNA聚合酶
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申请号: US13180225申请日: 2011-07-11
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公开(公告)号: US08765436B2公开(公告)日: 2014-07-01
- 发明人: Keith Bauer , Fred Reichert , Thomas W. Myers
- 申请人: Keith Bauer , Fred Reichert , Thomas W. Myers
- 申请人地址: US CA Pleasanton
- 专利权人: Roche Molecular Systems, Inc.
- 当前专利权人: Roche Molecular Systems, Inc.
- 当前专利权人地址: US CA Pleasanton
- 代理机构: Kilpatrick Townsend & Stockton LLP
- 主分类号: C12N9/00
- IPC分类号: C12N9/00 ; C12N15/54
摘要:
Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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