公开(公告)号：US08722380B2

公开(公告)日：2014-05-13

申请号：US13162688

申请日：2011-06-17

申请人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

发明人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

IPC分类号： C12N9/00 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Y207/07007 ,  C12Q2521/101

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US20130196382A1

公开(公告)日：2013-08-01

申请号：US13558760

申请日：2012-07-26

申请人： Keith Bauer ,  Thomas W. Myers ,  Fred Reichert ,  Shawn Suko

发明人： Keith Bauer ,  Thomas W. Myers ,  Fred Reichert ,  Shawn Suko

IPC分类号： C12N9/12

CPC分类号： C12N9/1276 ,  C12N9/1252 ,  C12N15/1096 ,  C12Q1/686 ,  C12Y207/07049

摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

公开(公告)号：US20120164641A1

公开(公告)日：2012-06-28

申请号：US13324705

申请日：2011-12-13

申请人： Keith Bauer ,  Nancy Schoenbrunner ,  Alison Tsan

发明人： Keith Bauer ,  Nancy Schoenbrunner ,  Alison Tsan

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6886 ,  C12Q1/6827 ,  C12Q2600/156

摘要： The invention comprises reagents and methods for detecting cancer-causing mutations in the human EGFR gene. Further, a method of detecting the mutations and a method of treatment are disclosed.

摘要翻译： 本发明包括用于检测人EGFR基因中的致癌突变的试剂和方法。 此外，公开了检测突变的方法和治疗方法。

公开(公告)号：US20070154914A1

公开(公告)日：2007-07-05

申请号：US11583606

申请日：2006-10-18

申请人： David Gelfand ,  Keith Bauer ,  Amar Gupta ,  Veeraiah Bodepudi ,  John Niemiec

发明人： David Gelfand ,  Keith Bauer ,  Amar Gupta ,  Veeraiah Bodepudi ,  John Niemiec

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12P19/34 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2565/301 ,  C12Q2525/186 ,  C12Q2521/101

摘要： The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

摘要翻译： 本发明提供包含包含2'-终止子核苷酸的封闭寡核苷酸的反应混合物。 当从寡核苷酸中除去2'-终止子核苷酸时，例如通过焦磷酸分解，封闭的寡核苷酸可以延伸。 反应混合物可用于许多其它应用中的各种核酸聚合和/或扩增测定中。 除了反应混合物之外，本发明还提供相关方法和反应混合物。

公开(公告)号：US20050098668A1

公开(公告)日：2005-05-12

申请号：US10703399

申请日：2003-11-07

申请人： Kevin Bailey ,  Keith Bauer

发明人： Kevin Bailey ,  Keith Bauer

IPC分类号： B05B3/00 ,  B05B3/18 ,  E01C23/16

CPC分类号： E01C23/163

摘要： The present invention provides an apparatus and method for applying road marking material to a pavement surface. The apparatus includes a vehicle having a holographic sight and at least one road marking material applicator mounted thereon. In accordance with the method, an operator of the vehicle steers the vehicle to a desired position on the pavement surface based upon aiming information observable through the holographic sight.

摘要翻译： 本发明提供一种将道路标线材料施加到路面上的装置和方法。 该装置包括具有全息瞄准器的车辆和安装在其上的至少一个道路标记材料施加器。 根据该方法，车辆的操作者基于通过全息视线可观察到的瞄准信息将车辆引导到路面上的期望位置。

公开(公告)号：US08765436B2

公开(公告)日：2014-07-01

申请号：US13180225

申请日：2011-07-11

申请人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

发明人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

IPC分类号： C12N9/00 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12Q1/6806 ,  C12Y207/07007

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

摘要翻译： 公开了相对于相应的未修饰的聚合酶具有增加的3'-错配鉴别的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。

公开(公告)号：US08722378B2

公开(公告)日：2014-05-13

申请号：US13162664

申请日：2011-06-17

申请人： Fred Reichert ,  Keith Bauer ,  Thomas W. Meyer

发明人： Fred Reichert ,  Keith Bauer ,  Thomas W. Meyer

IPC分类号： C12N9/00 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Y207/07007

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US08715991B2

公开(公告)日：2014-05-06

申请号：US13162684

申请日：2011-06-17

申请人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

发明人： Fred Reichert ,  Keith Bauer ,  Thomas W. Myers

IPC分类号： C12N9/12 ,  C12N15/54

CPC分类号： C12N9/1252 ,  C12N9/1276 ,  C12P19/34 ,  C12Y207/07007

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

摘要翻译： 公开了相对于相应的未修饰的聚合酶具有增加的3'-错配鉴别的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。

公开(公告)号：US20120208239A1

公开(公告)日：2012-08-16

申请号：US13180198

申请日：2011-07-11

申请人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

发明人： Keith Bauer ,  Fred Reichert ,  Thomas W. Myers

IPC分类号： C12N9/12 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/6806 ,  C12Y207/07007 ,  Y02P20/52

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.

公开(公告)号：US20120015405A1

公开(公告)日：2012-01-19

申请号：US13162674

申请日：2011-06-17

申请人： FRED REICHERT ,  KEITH BAUER ,  THOMAS W. MYERS

发明人： FRED REICHERT ,  KEITH BAUER ,  THOMAS W. MYERS

IPC分类号： C12P19/34 ,  C07H21/00 ,  C12N9/12

CPC分类号： C12N9/1252 ,  C12P19/34 ,  C12Q1/686 ,  C12Y207/07007

摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.