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公开(公告)号：US09017979B2
公开(公告)日：2015-04-28
申请号：US13443721
申请日：2012-04-10
申请人： Keith Bauer , Thomas W. Myers , Fred Reichert , Joseph San Filippo , Rachel Shahinian , Shawn Suko
发明人： Keith Bauer , Thomas W. Myers , Fred Reichert , Joseph San Filippo , Rachel Shahinian , Shawn Suko
CPC分类号： C12N9/1276 , C12N15/1096 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有增加的逆转录酶效率，错配耐受性，延伸速率和/或RT和聚合酶抑制剂的耐受性的DNA聚合酶。 聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如生产DNA聚合酶。
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公开(公告)号：US08735120B2
公开(公告)日：2014-05-27
申请号：US13162661
申请日：2011-06-17
CPC分类号： C12N9/1252 , C07K2319/00 , C12P19/34 , C12Q1/6827 , C12Q1/6848 , C12Q1/6858 , C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Y207/07007 , Y02P20/52 , C12Q2521/101 , C12Q2535/125
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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公开(公告)号：US20110312037A1
公开(公告)日：2011-12-22
申请号：US13162684
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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4.发明申请公开(公告)号：US20100006542A1
公开(公告)日：2010-01-14
申请号：US12300414
申请日：2007-05-11
申请人： Jordi Fred Reichert
发明人： Jordi Fred Reichert
CPC分类号： B05B1/24 , B30B5/04 , B30B15/062 , B44B5/026 , C23F1/02 , C23F1/08 , G03F7/16 , G03F7/2018
摘要： The invention relates to a method for producing a metallic pressed sheet or an endless strip, whereby a surface structure is produced by applying a mask for partial chemical passivation followed by chemical surface treatment. The invention also relates to a device for implementing said method. In order to significantly improve the reproducibility and the resolution of the mask obtained, the mask is produced from a UV-hardening lacquer which is applied by a suitable device. The resolution is improved by means of a nozzle matrix which sprays the UV-hardening lacquer in dots, the individual dots forming a pattern as a result of the overspraying thereof, thereby forming the mask to be produced. The UV-hardening lacquer is especially advantageous in that it can be removed from the surface very easily after the etching process, and enables a high reproducibility compared to the conventional screen printing methods.
摘要翻译： 本发明涉及一种金属压制片材或环形带材的制造方法，由此通过涂覆用于部分化学钝化的掩模化学表面处理来制造表面结构。 本发明还涉及一种用于实现所述方法的装置。 为了显着提高所获得的掩模的再现性和分辨率，掩模由通过合适的装置施加的UV硬化漆制成。 通过喷嘴矩阵改善分辨率，喷嘴矩阵以UV点喷射UV固化漆，各点由于其过度喷涂而形成图案，从而形成待制造的掩模。 UV硬化漆是特别有利的，因为它可以在蚀刻工艺之后非常容易地从表面除去，并且与传统的丝网印刷方法相比，能够实现高的再现性。
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5.发明授权公开(公告)号：US09529264B2
公开(公告)日：2016-12-27
申请号：US12300414
申请日：2007-05-11
申请人： Jordi Fred Reichert
发明人： Jordi Fred Reichert
CPC分类号： B05B1/24 , B30B5/04 , B30B15/062 , B44B5/026 , C23F1/02 , C23F1/08 , G03F7/16 , G03F7/2018
摘要： The invention relates to a method for producing a metallic pressed sheet or an endless strip, whereby a surface structure is produced by applying a mask for partial chemical passivation followed by chemical surface treatment. The invention also relates to a device for implementing said method. In order to significantly improve the reproducibility and the resolution of the mask obtained, the mask is produced from a UV-hardening lacquer which is applied by a suitable device. The resolution is improved by means of a nozzle matrix which sprays the UV-hardening lacquer in dots, the individual dots forming a pattern as a result of the overspraying thereof, thereby forming the mask to be produced. The UV-hardening lacquer is especially advantageous in that it can be removed from the surface very easily after the etching process, and enables a high reproducibility compared to the conventional screen printing methods.
摘要翻译： 本发明涉及一种金属压制片材或环形带材的制造方法，由此通过涂覆用于部分化学钝化的掩模化学表面处理来制造表面结构。 本发明还涉及一种用于实现所述方法的装置。 为了显着提高所获得的掩模的再现性和分辨率，掩模由通过合适的装置施加的UV硬化漆制成。 通过喷嘴矩阵改善分辨率，喷嘴矩阵以UV点喷射UV固化漆，各点由于其过度喷涂而形成图案，从而形成待制造的掩模。 UV硬化漆是特别有利的，因为它可以在蚀刻工艺之后非常容易地从表面除去，并且与传统的丝网印刷方法相比，能够实现高的再现性。
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公开(公告)号：US09090883B2
公开(公告)日：2015-07-28
申请号：US13558760
申请日：2012-07-26
申请人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
发明人： Keith Bauer , Thomas W. Myers , Fred Reichert , Shawn Suko
CPC分类号： C12N9/1276 , C12N9/1252 , C12N15/1096 , C12Q1/686 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
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公开(公告)号：US08759062B2
公开(公告)日：2014-06-24
申请号：US13162694
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer
发明人： Fred Reichert , Keith Bauer
CPC分类号： C12N9/1252 , C12Q1/6806 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰的聚合酶具有增加的3'-错配鉴别的突变型DNA聚合酶。 突变型聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如用于产生突变型DNA聚合酶。
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公开(公告)号：US08735121B2
公开(公告)日：2014-05-27
申请号：US13162674
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/686 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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公开(公告)号：US08722379B2
公开(公告)日：2014-05-13
申请号：US13162677
申请日：2011-06-17
申请人： Fred Reichert , Keith Bauer , Thomas W. Myers
发明人： Fred Reichert , Keith Bauer , Thomas W. Myers
CPC分类号： C12N9/1252 , C12Q1/6806 , C12Y207/07007
摘要： Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
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公开(公告)号：US20120258501A1
公开(公告)日：2012-10-11
申请号：US13443721
申请日：2012-04-10
申请人： Keith Bauer , Thomas Myers , Fred Reichert , Joseph San Filippo , Rachel Shahinian , Shawn Suko
发明人： Keith Bauer , Thomas Myers , Fred Reichert , Joseph San Filippo , Rachel Shahinian , Shawn Suko
CPC分类号： C12N9/1276 , C12N15/1096 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有增加的逆转录酶效率，错配耐受性，延伸速率和/或RT和聚合酶抑制剂的耐受性的DNA聚合酶。 聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如生产DNA聚合酶。
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