发明授权
- 专利标题: Oligopeptide sequence specifically bonding to phenylboronic acid group
- 专利标题(中): 特异性结合苯基硼酸基团的寡肽序列
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申请号: US13982755申请日: 2012-01-30
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公开(公告)号: US09175035B2公开(公告)日: 2015-11-03
- 发明人: Tomohiro Konno , Kazuhiko Ishihara , Hirokazu Nishida
- 申请人: Tomohiro Konno , Kazuhiko Ishihara , Hirokazu Nishida
- 申请人地址: JP Tokyo JP Tokyo
- 专利权人: HITACHI, LTD.,THE UNIVERSITY OF TOKYO
- 当前专利权人: HITACHI, LTD.,THE UNIVERSITY OF TOKYO
- 当前专利权人地址: JP Tokyo JP Tokyo
- 代理机构: Baker Botts, L.L.P.
- 优先权: JP2011-018163 20110131
- 国际申请: PCT/JP2012/051933 WO 20120130
- 国际公布: WO2012/105471 WO 20120809
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; A61K39/21 ; C07K1/22 ; C07K17/06 ; C07K17/08 ; C07K17/14 ; C12N9/02 ; G01N33/543
摘要:
An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.
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