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公开(公告)号：US20130344590A1
公开(公告)日：2013-12-26
申请号：US13982755
申请日：2012-01-30
CPC分类号： C07K1/22 , C07K17/06 , C07K17/08 , C07K17/14 , C12N9/0069 , G01N33/54353
摘要： An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.
摘要翻译： 本发明的目的在于提供作为固定化蛋白质分子的方法，能够控制待固定分子取向的蛋白质固定化方法和方法，而不需要复杂的工艺和化学物质即可稳定地分离 用于固定的组不影响蛋白质的活性和功能。 具体地说，本发明涉及一种用于固定分子的方法，包括通过将包含含羟基的氨基酸的标记肽序列附着在分子上而形成标记分子的步骤; 并使具有苯基硼酸基团的分子与标记分子接触，由具有苯基硼酸基团的分子捕获标记分子。
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公开(公告)号：US09175035B2
公开(公告)日：2015-11-03
申请号：US13982755
申请日：2012-01-30
IPC分类号： G01N33/53 , A61K39/21 , C07K1/22 , C07K17/06 , C07K17/08 , C07K17/14 , C12N9/02 , G01N33/543
CPC分类号： C07K1/22 , C07K17/06 , C07K17/08 , C07K17/14 , C12N9/0069 , G01N33/54353
摘要： An object of the present invention is to provide, as a method for immobilizing a protein molecule, protein immobilization method and means in which orientation of a molecule to be immobilized can be controlled, the molecule can be stably immobilized without a complicated process and a chemical group used for immobilization does not affect the activity and the function of the protein. Specifically, the present invention relates to a method for immobilizing a molecule including the steps of forming a labeled molecule by attaching a label peptide sequence comprising a hydroxyl group-containing amino acid to a molecule; and bringing a molecule having a phenylboronic acid group into contact with the labeled molecule, to capture the labeled molecule by the molecule having a phenylboronic acid group.
摘要翻译： 本发明的目的在于提供作为固定化蛋白质分子的方法，能够控制待固定分子取向的蛋白质固定化方法和方法，而不需要复杂的工艺和化学物质即可稳定地分离 用于固定的组不影响蛋白质的活性和功能。 具体地说，本发明涉及一种用于固定分子的方法，包括通过将包含含羟基的氨基酸的标记肽序列附着在分子上而形成标记分子的步骤; 并使具有苯基硼酸基团的分子与标记分子接触，由具有苯基硼酸基团的分子捕获标记分子。
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3.发明授权Slurry for slicing silicon ingot and method for slicing silicon ingot using the same 有权用于切片硅锭的浆料及其使用硅锭切片的方法公开(公告)号：US08075647B2
公开(公告)日：2011-12-13
申请号：US12441467
申请日：2006-10-20
CPC分类号： B28D5/007 , B28D5/045 , C09K3/1463 , Y02P70/179
摘要： The invention is a slurry for slicing a silicon ingot, containing a basic material, such as an alkali metal hydroxide, abrasive powder and water, in which the slurry contains the basic material in an amount of from 2 to 6% by mass and glycerin in an amount of from 25 to 55% by mass, based on a total mass of components of the slurry excluding the abrasive powder.
摘要翻译： 本发明是一种用于切割硅锭的浆料，其含有碱性物质如碱金属氢氧化物，磨料粉末和水，其中浆料含有2至6质量％的碱性物质，甘油在 相对于除研磨粉末以外的浆料的总质量，为25〜55质量％。
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公开(公告)号：US07927853B2
公开(公告)日：2011-04-19
申请号：US11473272
申请日：2006-06-23
CPC分类号： C12N9/93
摘要： It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.
摘要翻译： 旨在获得提高与DNA的结合能力和反应性的DNA连接酶，特别是耐热DNA连接酶，其与DNA的结合能力和反应性提高。 本发明提供了通过部分或完全缺失DNA连接酶的C末端螺旋部分获得的与DNA的结合能力和反应性提高的DNA连接酶突变体。 特别地，突变体来自耐热细菌。
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公开(公告)号：US20070037190A1
公开(公告)日：2007-02-15
申请号：US11473272
申请日：2006-06-23
CPC分类号： C12N9/93
摘要： It is intended to obtain DNA ligase improved in binding ability and reactivity with DNA, particularly thermostable DNA ligase improved in binding ability and reactivity with DNA. The present invention provides a DNA ligase mutant improved in binding ability and reactivity with DNA, which is obtained by partially or completely deleting a C-terminal helix portion of DNA ligase. Particularly, the mutant is derived from a thermostable bacterium.
摘要翻译： 旨在获得提高与DNA的结合能力和反应性的DNA连接酶，特别是耐热DNA连接酶，其与DNA的结合能力和反应性提高。 本发明提供了通过部分或完全缺失DNA连接酶的C末端螺旋部分获得的与DNA的结合能力和反应性提高的DNA连接酶突变体。 特别地，突变体来自耐热细菌。
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公开(公告)号：US08256407B2
公开(公告)日：2012-09-04
申请号：US12666721
申请日：2007-06-27
IPC分类号： B28D1/06
CPC分类号： B24B27/0633 , B23D57/0023 , B23D57/0061 , B28D5/045
摘要： To provide a multi-wire saw that, at the start of cutting of an ingot, prevents a wire from being displaced in grooves of guide rollers due to the wire being lifted. A wire is wound around a plurality of wire guide rollers to be positioned in a feeding direction of an ingot, and in this state, a wire-lifting restraining member that is a body of rotation and restrains the wire from being lifted by being brought into contact with the wire is disposed near the wire guide rollers.
摘要翻译： 为了提供一种多线锯，其在开始切割锭时，由于导线被提升而防止导线在导向辊的槽中移位。 电线缠绕在多个电线导向辊上以沿铸锭的进给方向定位，并且在该状态下，作为转动体的线材举升限制部件被限制，并且通过进入 与导丝线接触设置在导丝辊附近。
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公开(公告)号：US20090061481A1
公开(公告)日：2009-03-05
申请号：US12028372
申请日：2008-02-08
申请人： Hirokazu Nishida , Maiko Tanabe
发明人： Hirokazu Nishida , Maiko Tanabe
CPC分类号： C12N9/93
摘要： Disclosed is a modified hyperthermophilic DNA ligase having improved DNA binding ability and reactivity. The modified hyperthermophilic DNA ligase has an amino acid sequence corresponding to the amino acid sequence of a heat-resistant DNA ligase derived from a thermophilic bacterium, a hyperthermophilic bacterium, a thermophilic archaebacterium, or a hyperthermophilic archaebacterium, except with at least two of charged amino acids in the C-terminal helix region each being substituted by alanine, threonine, or serine residues.
摘要翻译： 公开了具有改进的DNA结合能力和反应性的改良的超嗜热DNA连接酶。 修饰的超嗜热DNA连接酶具有对应于源自嗜热细菌，嗜热细菌，嗜热古细菌或超嗜热古细菌的耐热DNA连接酶的氨基酸序列的氨基酸序列，除了至少两个带电氨基 C-末端螺旋区域中的酸各自被丙氨酸，苏氨酸或丝氨酸残基取代。
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公开(公告)号：US07981653B2
公开(公告)日：2011-07-19
申请号：US12028372
申请日：2008-02-08
申请人： Hirokazu Nishida , Maiko Tanabe
发明人： Hirokazu Nishida , Maiko Tanabe
CPC分类号： C12N9/93
摘要： Disclosed is a modified hyperthermophilic DNA ligase having improved DNA binding ability and reactivity. The modified hyperthermophilic DNA ligase has an amino acid sequence corresponding to the amino acid sequence of a heat-resistant DNA ligase derived from a thermophilic bacterium, a hyperthermophilic bacterium, a thermophilic archaebacterium, or a hyperthermophilic archaebacterium, except with at least two of the charged amino acids in the C-terminal helix region each being substituted by alanine, threonine, or serine residues.
摘要翻译： 公开了具有改进的DNA结合能力和反应性的改良的超嗜热DNA连接酶。 修饰的超嗜热DNA连接酶具有对应于源自嗜热细菌，嗜热细菌，嗜热古细菌或超嗜热古细菌的耐热DNA连接酶的氨基酸序列的氨基酸序列，除了至少两个带电 C-末端螺旋区域中的氨基酸各自被丙氨酸，苏氨酸或丝氨酸残基取代。
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公开(公告)号：US20100163009A1
公开(公告)日：2010-07-01
申请号：US12666721
申请日：2007-06-27
IPC分类号： B28D1/06
CPC分类号： B24B27/0633 , B23D57/0023 , B23D57/0061 , B28D5/045
摘要： To provide a multi-wire saw that, at the start of cutting of an ingot, prevents a wire from being displaced in grooves of guide rollers due to the wire being lifted. A wire is wound around a plurality of wire guide rollers to be positioned in a feeding direction of an ingot, and in this state, a wire-lifting restraining member that is a body of rotation and restrains the wire from being lifted by being brought into contact with the wire is disposed near the wire guide rollers.
摘要翻译： 为了提供一种多线锯，其在开始切割锭时，由于导线被提升而防止导线在导向辊的槽中移位。 电线缠绕在多个电线导向辊上以沿铸锭的进给方向定位，并且在该状态下，作为转动体的线材举升限制部件被限制，并且通过进入 与导丝线接触设置在导丝辊附近。
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公开(公告)号：US20090191595A1
公开(公告)日：2009-07-30
申请号：US12071900
申请日：2008-02-27
申请人： Maiko Tanabe , Hirokazu Nishida
发明人： Maiko Tanabe , Hirokazu Nishida
CPC分类号： C12Q1/6844 , C12Q1/6862 , C12Q2533/101 , C12Q2521/501
摘要： An object of the present invention is to provide a method for amplification of long nucleic acid, wherein the method allows nucleic acid fragments containing the same nucleotide sequence information to efficiently amplify at the same base length. The present invention relates to a method for amplification of long nucleic acid sequence, wherein the method uses primers being modified at the 5′ end with a phosphate group and performs a cooperative reaction using DNA polymerase and DNA ligase.
摘要翻译： 本发明的目的是提供一种用于扩增长核酸的方法，其中该方法允许含有相同核苷酸序列信息的核酸片段以相同的碱基长度有效扩增。 本发明涉及用于扩增长核酸序列的方法，其中该方法使用在5'端用磷酸基修饰的引物，并使用DNA聚合酶和DNA连接酶进行协同反应。
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