Invention Grant
- Patent Title: Bead incubation and washing on a droplet actuator
- Patent Title (中): 珠子孵化并在液滴致动器上进行洗涤
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Application No.: US14731740Application Date: 2015-06-05
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Publication No.: US09395361B2Publication Date: 2016-07-19
- Inventor: Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Richard B. Fair
- Applicant: Advanced Liquid Logic, Inc. , Duke University
- Applicant Address: US CA San Diego US NC Durham
- Assignee: ADVANCED LIQUID LOGIC, INC.,DUKE UNIVERSITY
- Current Assignee: ADVANCED LIQUID LOGIC, INC.,DUKE UNIVERSITY
- Current Assignee Address: US CA San Diego US NC Durham
- Agency: Ward and Smith, P.A.
- Agent David A. Fazzolare; Ryan K. Simmons
- Main IPC: G01N15/06
- IPC: G01N15/06 ; G01N33/00 ; G01N33/48 ; G01N33/543 ; B01F11/00 ; B01F13/00 ; B01L3/00 ; B03C1/02 ; B03C1/30 ; B03C5/00 ; G01N33/561 ; B03C1/28 ; B01L7/00 ; G01N35/10
Abstract:
The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
Public/Granted literature
- US20150268235A1 Bead Incubation and Washing on a Droplet Actuator Public/Granted day:2015-09-24
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