公开(公告)号：EP1477800A4

公开(公告)日：2005-08-17

申请号：EP03703116

申请日：2003-01-31

申请人： GL SCIENCES INC

发明人： KURODA IKUMA ,  TAKAHASHI KOUJI ,  NAKAMURA HIROSHI

IPC分类号： B01J20/282 ,  B01J20/287 ,  C07K1/20 ,  G01N30/02 ,  G01N30/08 ,  G01N30/34 ,  G01N30/46 ,  G01N30/60 ,  G01N30/88

CPC分类号： G01N30/88 ,  B01J20/0211 ,  B01J20/06 ,  B01J20/282 ,  B01J20/287 ,  B01J2220/54 ,  C07K1/20 ,  G01N30/02 ,  G01N30/08 ,  G01N30/34 ,  G01N30/466 ,  G01N30/468 ,  G01N30/6004 ,  G01N2030/085 ,  G01N2030/8813 ,  G01N2030/8818 ,  G01N2030/8831 ,  G01N2030/8836 ,  B01D15/325

摘要： It is intended to easily, conveniently and selectively detect, identify, extract, separate and purify a phosphorylated amino acid, a phosphorylated peptide or a phosphorylated protein from a sample containing the same. A method which comprises the step wherein a sample containing a phosphorylated amino acid, a phosphorylated peptide or a phosphorylated protein is passed through a reversed phase HPLC column provided with a titanium oxide column as a pretreatment column so as to allow the pretreatment column to sustain the phosphorylated amino acid, the phosphorylated peptide or the phosphorylated protein while allow the reversed phase HPLC column to sustain unphoshorylated amino acid, peptide or protein; the step of eluting the unphoshorylated amino acid, peptide or protein sustained by the reversed phase HPLC column; the subsequent step of eluting the phosphorylated amino acid, the phosphorylated peptide or the phosphorylated protein sustained by the pretreatment column with a buffer; and the step of detecting them.

公开(公告)号：EP1375673A4

公开(公告)日：2007-11-21

申请号：EP02705325

申请日：2002-03-19

申请人： JAPAN SCIENCE & TECH CORP ,  GL SCIENCES INC

发明人： KONDO TOSHIHIKO ,  ABE SHUZO ,  SHIKAMA NOBUYOSI ,  SHINTANI YUKIHIRO

IPC分类号： C12N15/09 ,  B01L7/00 ,  C12M1/00 ,  C12M1/38 ,  C12M1/40 ,  C12Q1/68 ,  G01N30/88 ,  C12N15/10

CPC分类号： C12Q1/6837 ,  B01L7/525 ,  B01L7/54 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2300/1827 ,  B01L2400/0487 ,  C12Q2565/137 ,  C12Q2527/101

摘要： A gene analysis method which comprises extracting a target nucleic acid from a biological sample (23), amplifying target DNA, supplying a reaction liquor (L) containing definite DNA into solid phase DNA probes (53) aligned in series or in parallel at a definite temperature and separating DNA complementary with the solid phase DNA probes, wherein a plural number of solid phase DNA probes, at least part of which can be controlled at the double-strand forming temperature of the test DNA, are provided and a reaction liquor containing the same or different DNAs after the amplification is supplied into the solid phase DNA probes.

摘要翻译： 一种基因分析方法，其特征在于，从生物试样（23）中提取目标核酸，扩增目标DNA，将含有确定的DNA的反应液（L）供给到串联或并联排列的固相DNA探针（53） 温度并分离与固相DNA探针互补的DNA，其中提供了多个固相DNA探针，其至少一部分可控制在测试DNA的双链形成温度，并且将含有 扩增后相同或不同的DNA被提供到固相DNA探针中。

公开(公告)号：EP2518489A4

公开(公告)日：2015-01-21

申请号：EP10845684

申请日：2010-02-12

申请人： GL SCIENCES INC

发明人： SOTOMARU KATSUHIKO ,  ZHOU XIAO-JING ,  YAMASAKI KAZUHIKO ,  SATO ATSUSHI

IPC分类号： G01N30/08 ,  F25B9/14 ,  G01N1/40 ,  G01N30/00 ,  G01N30/04

CPC分类号： G01N30/12 ,  F25B9/14 ,  G01N1/40 ,  G01N30/465 ,  G01N30/7206 ,  G01N2001/4033 ,  G01N2030/085 ,  G01N2030/121 ,  G01N2030/122 ,  G01N2030/128 ,  G01N2030/884

公开(公告)号：EP1626266A4

公开(公告)日：2012-02-01

申请号：EP04732443

申请日：2004-05-12

申请人： GL SCIENCES INC

发明人： IWANO TAKESHI ,  YAMADA TSUYOSHI ,  ZHOU XIAOJING

IPC分类号： G01N27/62 ,  G01N30/74 ,  G01N21/05 ,  G01N21/17 ,  G01N27/447

CPC分类号： G01N30/74 ,  G01N21/05 ,  G01N2021/0346 ,  G01N2030/746

公开(公告)号：EP1329714A4

公开(公告)日：2008-11-19

申请号：EP01970200

申请日：2001-09-25

申请人： GL SCIENCES INC

发明人： FURUNO MASAHIRO ,  OHIRA MASAYOSHI ,  FURUSHIMA NORITAKA ,  HAMADA TAKAYUKI ,  HIRAMATSU YUKA ,  SHINTANI YUKIHIRO

IPC分类号： G01N1/40 ,  G01N30/08 ,  G01N30/52 ,  G01N30/48

CPC分类号： G01N30/08 ,  B01J2220/54 ,  G01N1/405 ,  G01N2030/525 ,  G01N2030/528 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method of collecting and concentrating a specimen capable of holding measured substances in the pin holes of a porous body inside a column and concentrating the substances by allowing a measured substance-containing specimen to be passed through the pin holes by using the column having the porous body of open structure formed inside a fine tube trough the full diameter and proper length of the fine tube so that pre-treatments forthe adjustment of the specimen such as extraction and concentration of objective components can be performed very easily in short time in a chromatography analysis, comprising the steps of performing the said process as a micro trap in the pre-treatment of the chromatography analysis and leading the process to an analysis means, leading a specimen band to a separation column after reducing the width thereof when leading the specimen into a liquid chromatography, and collecting atmospheric substances such as gaseous substances contained in the air into a collecting tube by using the collecting tube not using a power such as a motor, having a very simple structure as a so-called passive sampler, capable of being produced at a low cost, and capable of uniformly collecting samples by simultaneously usign a large number of tubes to increase the number of measurements, and forming therein, as collecting agent, the porous body of open structure having less ventilating pressure loss and less resistance by adsorbent because if increased surface area, and formed of inorganic, organic, or hybrid polymer partly or wholly in the raw tube thereof.

公开(公告)号：EP2336764A4

公开(公告)日：2011-09-28

申请号：EP09811272

申请日：2009-09-01

申请人： GL SCIENCES INC

发明人： ZHOU XIAOJING ,  UZU HIDEYUKI

IPC分类号： G01N30/02 ,  G01N30/20 ,  G01N30/24 ,  G01N30/54 ,  G01N30/60

CPC分类号： G01N30/24 ,  G01N30/6047

公开(公告)号：EP1785719A4

公开(公告)日：2008-10-08

申请号：EP04772536

申请日：2004-08-31

申请人： GL SCIENCES INC

发明人： YAMADA TSUYOSHI ,  ZHOU XIAOJING

IPC分类号： G01N21/27 ,  G01J1/16

CPC分类号： G01N21/274 ,  G01J3/42

摘要： A cell sample being inserted in an optical system is irradiated with light from a light source. Transmitted light which has passed through the cell is detected. Transmitted light on the side of a reference being inserted in the optical system is detected. Such detection signals are adjusted, and the output of the detector is calculated, based on the detection signals. The detection signals on the sample side and the reference side are both adjusted corresponding to the optimum output level of the detector. A change quantity of a ratio of the detected signals on the sample side to those on the reference side, which comprises detector output, is controlled, and a baseline noise is lowered.

公开(公告)号：EP1719816A4

公开(公告)日：2007-06-06

申请号：EP04710506

申请日：2004-02-12

申请人： GL SCIENCES INC

发明人： ABUDOKIRIM ABUDOGUPUR ,  OHIRA MASAYOSHI ,  OKUSA KENSUKE ,  SETO NOBUO ,  FURUNO MASAHIRO

IPC分类号： C12N15/10 ,  C07H21/00 ,  C12P19/34

CPC分类号： C07H21/00 ,  C12N15/1006

摘要： A simplified mechanism and method for highly efficiently separating and purifying a nucleic acid (in particular a fragmental DNA) at a high reproducibility without resort to elution with a salt at a high concentration or elution/purification, thereby giving a fragment having a high purity. This mechanism, which aims at purifying a nucleic acid (in particular a fragmental DNA) with the use of a monolith structure, is provided with a monolith structure made of glass or silica, namely, a porous integral structure having small pores penetrating thorough from the top to the bottom and the penetrating pores being in a size fitting for the nucleic acid size of from 35 bp (mer) to 100 Kbp (mer).

公开(公告)号：EP1382963A4

公开(公告)日：2004-07-14

申请号：EP02722709

申请日：2002-04-19

申请人： GL SCIENCES INC

发明人： HIRAMATSU YUKA ,  YAMASAKI KAZUHIKO ,  FURUNO MASAHIRO

IPC分类号： B01D15/00 ,  B01J20/28 ,  B01L3/02 ,  G01N1/34 ,  G01N1/40 ,  G01N30/00 ,  G01N30/18 ,  G01N30/08 ,  B01D15/08 ,  B01J20/04 ,  B01J20/10

CPC分类号： G01N1/405 ,  B01D15/00 ,  B01D15/08 ,  B01J20/28042 ,  B01J20/28054 ,  B01J20/28092 ,  B01J2220/64 ,  B01L3/022 ,  B01L3/0275 ,  B01L2200/0631 ,  G01N1/40 ,  G01N30/18 ,  G01N2030/009

摘要： A method for extracting a component in a sample in solid phase in order to provide means effective for pretreating a sample being introduced to a chromatograph. The method comprises a step for providing a needle at the forward end of a syringe having a tube and a plunger slidable in the tube, forming a porous body of so-called monolithic structure in the needle at least over a proper length and the entire diameter and then inserting the needle into a sample, a step for passing the sample through the needle and holding a target component in the porous body, and a step for separating the target component from the interior of the porous body.

公开(公告)号：EP2246299A4

公开(公告)日：2012-07-04

申请号：EP08710958

申请日：2008-02-01

申请人： GL SCIENCES INC

发明人： MIYAZAKI SHOTA ,  TERASHIMA HIROYUKI ,  NYUDO MASAHIKO ,  OHIRA MASAYOSHI ,  MORISATO KEI ,  FURUNO MASAHIRO

IPC分类号： C01B33/12 ,  B01D15/08 ,  B01J20/10 ,  B01J20/28 ,  B01J20/32 ,  G01N30/88

CPC分类号： B01D15/22 ,  B01D15/20 ,  B01J20/103 ,  B01J20/28042 ,  B01J20/283 ,  B01J20/3257 ,  B01J2220/54 ,  B01J2220/82 ,  B01J2220/825 ,  G01N30/50 ,  G01N30/6052 ,  G01N2030/528