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    METHOD OF QUANTIFYING NEUROTOXIN
    6.
    发明授权
    METHOD OF QUANTIFYING NEUROTOXIN 无效
    方法定量NEURO毒素

    公开(公告)号：EP2015065B1

    公开(公告)日：2011-10-05

    申请号：EP06745899.2

    申请日：2006-04-28

    申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute ,  JAPAN as represented by DIRECTOR GENERAL OF NATIONAL INSTITUTE OF INFECTIOUS DISEASES

    发明人： HARAKAWA, Tetsuhiro ,  NAKANO, Hirotoshi ,  TORII, Yasushi ,  OKUDA, Sachio ,  KAJI, Ryuji ,  SAKAMOTO, Takashi ,  TAKAHASHI, Motohide ,  ISHIDA, Setsuji

    IPC分类号： G01N33/15 ,  G01N33/50

    CPC分类号： G01N33/5088 ,  G01N33/94

    摘要： A method of quantitatively measuring the muscle relaxant action of a neurotoxin. More specifically speaking, a method of quantifying the efficacy (titer and/or diffusion reaction) of a neurotoxin based on the degree of the muscle relaxant action of a neurotoxin originating in a bacterium belonging to the genus Clostridium, which is characterized by comprising: (a) administering the neurotoxin to one hinder leg of a nonhuman mammal; (b) electrically stimulating the nonhuman mammal; (c) measuring the compound muscle action potential (CMAP) caused by the contraction of muscles in the hinder leg to which the neurotoxin has been administered and/or muscles in the other hinder leg to which the neurotoxin has not been administered by using an electromyograph; and (d) taking out the amplitude data from the compound muscle action potential (CMAP) obtained by the measurement in the step (c) and analyzing the degree of a decrease in the amplitude to thereby quantify the efficacy of the muscle relaxant action by the neurotoxin. Although mouse LD50 that has been employed as a titer unit of the botulin toxin allows the measurement only at a several unit level, the above-described method of quantifying the efficacy of a neurotoxin enables the measurement at 0.01 to 1 unit level. That is, this method is excellent in sensitivity, reproducibility and accuracy.

    SRSV DETECTION KIT
    10.
    发明公开
    SRSV DETECTION KIT 有权
    SRSV-NACHWEISKIT

    公开(公告)号：EP1186890A1

    公开(公告)日：2002-03-13

    申请号：EP00940803.0

    申请日：2000-06-22

    申请人： Japan as represented by Director General of National Institute of Infectious Diseases ,  DENKA SEIKEN Co., Ltd.

    发明人： TAKEDA, Naokazu, Nat. Inst. of Infectious Diseases ,  NATORI, Katsuro, Nat. Inst. of Infectious Diseases ,  MIYAMURA, Tatsuo, Nat. Inst. Infectious Diseases ,  KAMATA, Kunio, Denka Seiken Co. Ltd. ,  SATO, Toshinori, Denka Seiken Co. Ltd. ,  SATO, Seiya, Denka Seiken Co. Ltd.

    IPC分类号： G01N33/569 ,  C12N15/40

    CPC分类号： C07K16/10 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/16021 ,  C12N2770/16022 ,  C12N2770/16023 ,  G01N33/56983 ,  G01N2333/08

    摘要： This invention relates to an SRSV detection kit comprising all antibodies against SRSV-related virus constituting peptides selected from the following peptide groups (a) to (k), respectively: (a) a peptide having an amino acid sequence represented by SEQ ID NO: 1, and the like, (b) a peptide having an amino acid sequence represented by SEQ ID NO: 2, and the like, (c) a peptide having an amino acid sequence represented by SEQ ID NO: 3, and the like, (d) a peptide having an amino acid sequence represented by SEQ ID NO: 4, and the like, (e) a peptide having an amino acid sequence represented by SEQ ID NO: 5, and the like, (f) a peptide having an amino acid sequence represented by SEQ ID NO: 6, and the like, (g) a peptide having an amino acid sequence represented by SEQ ID NO: 7, and the like, (h) a peptide having an amino acid sequence represented by SEQ ID NO: 8, and the like, (i) a peptide having an amino acid sequence represented by SEQ ID NO: 9, and the like, (j) a peptide having an amino acid sequence represented by SEQ ID NO: 10, and the like, and (k) a peptide having an amino acid sequence represented by SEQ ID NO: 11, and the like.
    Use of the kit makes it possible to detect most SRSV-related viruses and further to distinguish their serotypes and genogroups, easily and surely.

    摘要翻译： 本发明涉及SRSV检测试剂盒，其包含构成分别选自以下肽组（a）至（k）的肽的SRSV相关病毒的所有抗体：（a）具有SEQ ID NO： 1等，（b）具有SEQ ID NO：2所示的氨基酸序列的肽等，（c）具有SEQ ID NO：3所示的氨基酸序列的肽等， （d）具有SEQ ID NO：4所示的氨基酸序列的肽等，（e）具有SEQ ID NO：5所示的氨基酸序列的肽等，（f）具有 由SEQ ID NO：6表示的氨基酸序列等，（g）具有由SEQ ID NO：7表示的氨基酸序列的肽等，（h）具有由 SEQ ID NO：8等，（i）具有SEQ ID NO：9所示的氨基酸序列的肽等，（j）具有 由SEQ ID NO：10表示的微型酸序列等，和（k）具有SEQ ID NO：11所示的氨基酸序列的肽等。 使用该试剂盒可以检测大多数与SRSV相关的病毒，并进一步区分其血清型和基因组，轻松而可靠。