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公开(公告)号：EP2331558A2
公开(公告)日：2011-06-15
申请号：EP09815409.9
申请日：2009-09-22
发明人： DELLINGER, Douglas, J. , MYERSON, Joel , SIERZCHALA, Agnieszka , DELLINGEN, Geraldine, F. , TIMAR, Zoltan
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A nucleoside monomer that is protected by a thionocarbamate protecting group is provided, as well as a method for making a polynucleotide that uses the same. Also provided is a polynucleotide synthesis method that employs a diamine to deprotect a protected polynucleotide.
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公开(公告)号：EP4400584A3
公开(公告)日：2024-10-16
申请号：EP24165113.2
申请日：2015-12-03
IPC分类号： C12N15/113 , C07H21/00 , C12N9/22
摘要： The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
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公开(公告)号：EP3818066A1
公开(公告)日：2021-05-12
申请号：EP18745793.2
申请日：2018-07-05
发明人： MYERSON, Joel , CHEN, Siyuan
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公开(公告)号：EP2331558B1
公开(公告)日：2018-09-12
申请号：EP09815409.9
申请日：2009-09-22
发明人： DELLINGER, Douglas, J. , MYERSON, Joel , SIERZCHALA, Agnieszka , DELLINGEN, Geraldine, F. , TIMAR, Zoltan
IPC分类号： C07H19/00 , C07H19/067 , C07H19/167 , C07H21/02 , C07H21/00
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A method of deprotecting a solid support bound polynucleotide comprising at least one 2′-protected ribonucleotide in which a step of contacting the polynucleotide with a composition comprising a diamine is performed under conditions sufficient to deprotect and cleave the polynucleotide which remains retained on the solid support.
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5.发明公开CHEMICALLY MODIFIED GUIDE RNAS FOR CRISPR/CAS-MEDIATED GENE REGULATION 审中-公开用于CRISPR / CAS介导的基因调控的化学修饰的指导RNA
公开(公告)号：EP3280803A1
公开(公告)日：2018-02-14
申请号：EP16720220.9
申请日：2016-04-05
发明人： PORTEUS, Matthew H. , HENDEL, Ayal , CLARK, Joe , BAK, Rasmus O. , RYAN, Daniel E. , DELLINGER, Douglas J. , KAISER, Robert , MYERSON, Joel
CPC分类号： C12N15/11 , C12N9/22 , C12N9/96 , C12N15/111 , C12N15/907 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/321 , C12N2310/346 , C12N2310/531 , C12N2320/51
摘要： Provided herein are methods for inducing CRISPR/Cas-based gene regulation (e.g., genome editing or gene expression) of a target nucleic acid (e.g., target DNA or target RNA) in a cell. The methods include using modified single guide RNAs (sgRNAs) that enhance gene regulation of the target nucleic acid in a primary cell for use in ex vivo therapy or in a cell in a subject for use in in vivo therapy. Additionally, provided herein are methods for preventing or treating a genetic disease in a subject by administering a sufficient amount of a modified sgRNA to correct a mutation in a target gene associated with the genetic disease.
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公开(公告)号：EP3227447A1
公开(公告)日：2017-10-11
申请号：EP15866342.7
申请日：2015-12-03
发明人： RYAN, Daniel, E. , DELLINGER, Douglas, J. , SAMPSON, Jeffrey, R. , KAISER, Robert , MYERSON, Joel
IPC分类号： C12N15/113 , C07H21/00 , C12N9/22
CPC分类号： C12N15/111 , C07H21/02 , C12N9/22 , C12N15/113 , C12N15/907 , C12N2310/10 , C12N2310/20 , C12N2310/312 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/323 , C12N2310/3231 , C12N2310/333 , C12N2310/335 , C12N2310/346 , C12N2310/3517 , C12N2310/531 , C12N2320/51 , C12N2320/53 , C12N2330/31 , C12Q1/6876 , C12N2310/3521 , C12N2310/3533
摘要： The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
摘要翻译： 本发明涉及修饰的指导RNA及其在聚集的，规则间隔的短回文重复序列（CRISPR）/ CRISPR相关（Cas）系统中的用途。
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公开(公告)号：EP3668883A1
公开(公告)日：2020-06-24
申请号：EP18778581.1
申请日：2018-08-17
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公开(公告)号：EP2160396B1
公开(公告)日：2018-11-21
申请号：EP08755268.3
申请日：2008-05-09
发明人： DELLINGER, Douglas J. , SIERZCHALA, Agnieska , TURNER, John , MYERSON, Joel , KUPIHAR, Zoltan , FERREIRA, Fernando , CARUTHERS, Marvin H.
IPC分类号： C07H19/073 , C07H19/16 , C07H21/02 , A61K31/7072 , A61K31/7076 , A61K31/7088
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2'-protected nucleoside monomers that are protected at the 2'-site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
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公开(公告)号：EP2160396A2
公开(公告)日：2010-03-10
申请号：EP08755268.3
申请日：2008-05-09
发明人： DELLINGER, Douglas J. , SIERZCHALA, Agnieska , TURNER, John , MYERSON, Joel , KUPIHAR, Zoltan , FERREIRA, Fernando , CARUTHERS, Marvin H.
IPC分类号： C07H19/073 , C07H19/16 , C07H21/02 , A61K31/7072 , A61K31/7076 , A61K31/7088
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2'-protected nucleoside monomers that are protected at the 2'-site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括在2'位置用硫代碳保护基如式（I）保护的2'保护的核苷单体。 感兴趣的硫代碳保护基团包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基团以及硫代氨基甲酸酯保护基团。 本发明的各方面还包括包含本发明保护基团的核酸，以及使用本发明的保护基团合成核酸的方法。式（I）。
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公开(公告)号：EP4400584A2
公开(公告)日：2024-07-17
申请号：EP24165113.2
申请日：2015-12-03
IPC分类号： C12N9/22
CPC分类号： C12N9/22 , C07H21/02 , C12N2310/1020130101 , C12N2310/31220130101 , C12N2310/32320130101 , C12N2310/33320130101 , C12N2310/34620130101 , C12N2310/351720130101 , C12N2320/5120130101 , C12N2320/5320130101 , C12N2330/3120130101 , C12N2310/2020170501 , C12N15/113 , C12N2310/31520130101 , C12N2310/323120130101 , C12N2310/33520130101 , C12N2310/53120130101 , C12N15/111
摘要： The present invention relates to modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems.
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