SYSTEM AND METHOD FOR PRODUCING TARGET BIOLOGICAL SUBSTANCES

    公开(公告)号:EP3712612A1

    公开(公告)日:2020-09-23

    申请号:EP20174012.3

    申请日:2013-11-20

    IPC分类号: G01N33/48 B01L3/00

    摘要: A biomimetic microfluidic system and a method are provided for producing target biological substances. The system comprises a first channel extending from a first input to a first output along a longitudinal dimension. The first channel is configured to receive at least one first biological composition at a first channel flow rate. The at least one first biological composition comprises a biological source material capable of producing target biological substances. The system further comprises a second channel substantially parallel to the first channel, extending from a second input to a second output along the longitudinal dimension. The second channel is configured to selectively receive at least one second biological composition at a second channel flow rate. The system further comprises a separation barrier between the first channel and the second channel. The separation barrier has a plurality of microchannels forming a fluid communication path between the first and second channels.

    SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING
    4.
    发明公开
    SYSTEM AND METHOD FOR A BIOMIMETIC FLUID PROCESSING 审中-公开
    系统VERFAHREN ZUR BEHANDLUNG EINES BIOMIMETISCHEN FLUIDS

    公开(公告)号:EP2941642A1

    公开(公告)日:2015-11-11

    申请号:EP13870350.9

    申请日:2013-11-20

    IPC分类号: G01N33/48 G06F19/12

    摘要: A system and method are provided for harvesting target biological substances. The system includes a substrate and a first and second channel formed in the substrate. The channels longitudinally extending substantially parallel to each other. A series of gaps extend from the first channel to the second channel to create a fluid communication path passing between a series of columns with the columns being longitudinally separated by a predetermined separation distance. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate. The sources are configured to create a differential between the first and second channel flow rates to generate physiological shear rates along the second channel that are bounded within a predetermined range.

    摘要翻译: 提供了用于收集目标生物物质的系统和方法。 该系统包括基板和形成在基板中的第一和第二通道。 纵向延伸的通道彼此平行。 一系列间隙从第一通道延伸到第二通道,以产生通过一系列柱之间的流体连通路径,其中柱纵向间隔预定间隔距离。 该系统还包括配置成以第一通道流速选择性地将第一生物组合物引入第一通道的第一源,以及配置成以第二通道流速将第二生物组合物选择性地引入第二通道的第二源。 源被配置为在第一和第二通道流速之间产生差分,以产生沿着第二通道的生理剪切速率,所述生理剪切速率限制在预定范围内。

    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS
    5.
    发明公开
    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS 审中-公开
    系统VERFAHREN ZUR BARCODIERUNG VONNUKLEINSÄUREN

    公开(公告)号:EP3134536A1

    公开(公告)日:2017-03-01

    申请号:EP15783629.7

    申请日:2015-04-17

    IPC分类号: C12P19/34

    摘要: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include "barcodes" or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

    摘要翻译: 本发明一般涉及微流体和标记的核酸。 例如,某些方面通常涉及用于在微流体液滴内标记核酸的系统和方法。 在一组实施方案中,核酸可以包括可用于将液滴中的核酸与另一个液滴中的核酸区分开的“条形码”或唯一序列,例如甚至在核酸汇集在一起​​之后。 在一些情况下,可以使用颗粒将独特的序列并入单独的液滴中并连接到液滴内的核酸(例如从裂解的细胞中释放)。 在一些情况下,条形码可以用于区分数十,数百甚至数千个核酸,例如由不同细胞或其他来源产生的核酸。

    SYSTEMS AND METHODS FOR BIOMIMETIC FLUID PROCESSING
    7.
    发明公开
    SYSTEMS AND METHODS FOR BIOMIMETIC FLUID PROCESSING 审中-公开
    系统VERFAHREN ZUR VERARBEITUNG EINES BIOMIMETISCHEN FLUIDS

    公开(公告)号:EP3126484A1

    公开(公告)日:2017-02-08

    申请号:EP15773878.2

    申请日:2015-03-30

    IPC分类号: C12M1/00 C12M3/08 B01L3/00

    摘要: Systems and methods generating physiologic models that can produce functional biological substances are provided. In some aspects, a system includes a substrate and a first and second channel formed therein. The channels extend longitudinally and are substantially parallel to each other. A series of apertures extend between the first channel and second channel to create a fluid communication path passing through columns separating the channels that extends further along the longitudinal dimension than other dimensions. The system also includes a first source configured to selectively introduce into the first channel a first biological composition at a first channel flow rate and a second source configured to selectively introduce into the second channel a second biological composition at a second channel flow rate, wherein the first channel flow rate and the second channel flow rate create a differential configured to generate physiological shear rates within a predetermined range in the channels.

    摘要翻译: 提供了能产生功能生物物质的生理模型的系统和方法。 在一些方面,一种系统包括基板和形成在其中的第一和第二通道。 通道纵向延伸并且基本上彼此平行。 一系列孔口在第一通道和第二通道之间延伸以产生通过分隔沿纵向尺寸进一步延伸的通道的柱的流体连通路径。 该系统还包括被配置为以第一通道流速选择性地将第一生物组合物引入第一通道的第一源和被配置为以第二通道流速将第二生物组合物选择性地引入第二通道的第二源,其中 第一通道流速和第二通道流速产生构造成在通道中的预定范围内产生生理剪切速率的差速器。