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    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS
    2.
    发明公开
    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS 审中-公开
    系统VERFAHREN ZUR BARCODIERUNG VONNUKLEINSÄUREN

    公开(公告)号：EP3134536A1

    公开(公告)日：2017-03-01

    申请号：EP15783629.7

    申请日：2015-04-17

    申请人： President and Fellows of Harvard College ,  Vilnius University

    发明人： WEITZ, David, A. ,  KLEIN, Allon, Moshe ,  AKARTUNA, Ilke ,  MAZUTIS, Linas ,  KIRSCHNER, Marc, W.

    IPC分类号： C12P19/34

    摘要： The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include "barcodes" or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

    摘要翻译： 本发明一般涉及微流体和标记的核酸。 例如，某些方面通常涉及用于在微流体液滴内标记核酸的系统和方法。 在一组实施方案中，核酸可以包括可用于将液滴中的核酸与另一个液滴中的核酸区分开的“条形码”或唯一序列，例如甚至在核酸汇集在一起​​之后。 在一些情况下，可以使用颗粒将独特的序列并入单独的液滴中并连接到液滴内的核酸（例如从裂解的细胞中释放）。 在一些情况下，条形码可以用于区分数十，数百甚至数千个核酸，例如由不同细胞或其他来源产生的核酸。