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    METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN
    3.
    发明公开
    METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN 审中-公开
    生产糖基化免疫球蛋白的方法

    公开(公告)号：EP3202785A1

    公开(公告)日：2017-08-09

    申请号：EP17155939.6

    申请日：2010-10-25

    申请人： F. Hoffmann-La Roche AG ,  Chugai Seiyaku Kabushiki Kaisha

    发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

    IPC分类号： C07K16/00

    CPC分类号： C12P21/005 ,  C07K16/2866 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/76

    摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

    摘要翻译： 本文报道了用于产生免疫球蛋白的方法，其包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）在培养基中培养真核细胞，其中培养基中可获得的葡萄糖的量 每时间单位保持恒定并且限制为小于每单位时间培养基中细胞可以最大限度利用的量的80％，以及c）从培养物中回收免疫球蛋白。

    CONJUGATES OF ERYTHROPOIETIN (EPO) WITH POLYETHYLENE GLYCOL (PEG)
    4.
    发明授权
    CONJUGATES OF ERYTHROPOIETIN (EPO) WITH POLYETHYLENE GLYCOL (PEG) 有权
    促红细胞生成素偶联物（EPO）与聚乙二醇（PEG）

    公开(公告)号：EP1345628B1

    公开(公告)日：2011-04-13

    申请号：EP01984811.8

    申请日：2001-12-08

    申请人： F. Hoffmann-La Roche AG

    发明人： BURG, Josef ,  ENGEL, Alfred ,  FRANZE, Reinhard ,  HILGER, Bernd ,  SCHURIG, Hartmut, Ernst ,  TISCHER, Wilhelm ,  WOZNY, Manfred

    IPC分类号： A61K47/48

    CPC分类号： A61K47/62 ,  A61K47/60

    摘要： The present invention refers to conjugates of erythropoietin with poly(ethylene glycol) comprising an erythropoietin glycoprotein having an N-terminal α-amino group and having the in vivo biological activity of causing bone marrow cells to increase production of reticulocytes and red blood cells and selected from the group consisting of human erythropoietin and analogs thereof which have sequence of human erythropoietin modified by the addition of from 1 to 6 glycosylation sites or a rearrangement of at least one glycosylation site; said glycoprotein being covalently linked to one poly(ethylene glycol) group of the formula -CO-(CH2)x-(OCH2CH2)m-OR with the -CO of the poly(ethylene glycol) group forming an amide bond with said N-terminal α-amino group; wherein R is lower alkyl; x is 2 or 3; and m is from about 450 to about 1350.

    CONJUGATES OF ERYTHROPOIETIN (PEP) WITH POLYETHYLENE GLYCOL (PEG)
    5.
    发明公开
    CONJUGATES OF ERYTHROPOIETIN (PEP) WITH POLYETHYLENE GLYCOL (PEG) 有权
    促红细胞生成素偶联物（EPO）与聚乙二醇（PEG）

    公开(公告)号：EP1345628A2

    公开(公告)日：2003-09-24

    申请号：EP01984811.8

    申请日：2001-12-08

    申请人： F. Hoffmann-La Roche AG

    发明人： BURG, Josef ,  ENGEL, Alfred ,  FRANZE, Reinhard ,  HILGER, Bernd ,  SCHURIG, Hartmut, Ernst ,  TISCHER, Wilhelm ,  WOZNY, Manfred

    IPC分类号： A61K47/48

    CPC分类号： A61K47/62 ,  A61K47/60

    摘要： The present invention refers to conjugates of erythropoietin with poly(ethylene glycol) comprising an erythropoietin glycoprotein having an N-terminal α-amino group and having the in vivo biological activity of causing bone marrow cells to increase production of reticulocytes and red blood cells and selected from the group consisting of human erythropoietin and analogs thereof which have sequence of human erythropoietin modified by the addition of from 1 to 6 glycosylation sites or a rearrangement of at least one glycosylation site; said glycoprotein being covalently linked to one poly(ethylene glycol) group of the formula -CO-(CH2)x-(OCH2CH2)m-OR with the -CO of the poly(ethylene glycol) group forming an amide bond with said N-terminal α-amino group; wherein R is lower alkyl; x is 2 or 3; and m is from about 450 to about 1350.

    METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN
    6.
    发明公开
    METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN 有权
    VERFAHREN ZUR HERSTELLUNG VON GLYCOSYLIERTEM IMMUNOGLOBULIN

    公开(公告)号：EP2493922A1

    公开(公告)日：2012-09-05

    申请号：EP10771093.1

    申请日：2010-10-25

    申请人： F.Hoffmann-La Roche AG ,  Chugai Seiyaku Kabushiki Kaisha

    发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

    IPC分类号： C07K16/00

    CPC分类号： C12P21/005 ,  C07K16/2866 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/76

    摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

    摘要翻译： 本文报道了用于产生免疫球蛋白的方法，包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）培养培养基中的真核细胞，其中培养基中可用的葡萄糖量 每个时间单位保持恒定并且被限制在小于每单位培养基中的细胞可最大限度地利用的量的80％，和c）从培养物中回收免疫球蛋白。