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1.发明公开METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN 有权VERFAHREN ZUR HERSTELLUNG VON GLYCOSYLIERTEM IMMUNOGLOBULIN
公开(公告)号：EP2493922A1
公开(公告)日：2012-09-05
申请号：EP10771093.1
申请日：2010-10-25
发明人： FRANZE, Reinhard , HIRASHIMA, Chikashi , LINK, Thomas , TAKAGI, Yoshinori , TAKUMA, Shinya , TSUDA, Yuriko
IPC分类号： C07K16/00
CPC分类号： C12P21/005 , C07K16/2866 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/76
摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.
摘要翻译： 本文报道了用于产生免疫球蛋白的方法，包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）培养培养基中的真核细胞，其中培养基中可用的葡萄糖量 每个时间单位保持恒定并且被限制在小于每单位培养基中的细胞可最大限度地利用的量的80％，和c）从培养物中回收免疫球蛋白。
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公开(公告)号：EP2493922B1
公开(公告)日：2017-02-15
申请号：EP10771093.1
申请日：2010-10-25
发明人： FRANZE, Reinhard , HIRASHIMA, Chikashi , LINK, Thomas , TAKAGI, Yoshinori , TAKUMA, Shinya , TSUDA, Yuriko
CPC分类号： C12P21/005 , C07K16/2866 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/76
摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.
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3.发明公开PEPTIDE-CONTAINING CULTURE MEDIUM FOR CULTURING ANIMAL CELL 有权PEPTIDHALTIGES KULTURMEDIUM ZUR KULTIVIERUNG VON TIERZELLEN
公开(公告)号：EP2351833A1
公开(公告)日：2011-08-03
申请号：EP09823558.3
申请日：2009-10-27
发明人： KATAYAMA, Satoshi , KISHISHITA, Shouhei , KODAIRA, Kunihiko , SADAMITSU, Makoto , TAKAGI, Yoshinori , MATSUDA, Hiroki
CPC分类号： C12P21/02 , C07K5/081 , C07K5/0812 , C07K16/00 , C07K16/303 , C07K2317/24 , C12N5/0037 , C12N2501/998 , C12N2510/02
摘要： Disclosed is a culture medium for culturing an animal cell, which is characterized by containing a peptide comprising, as a constituent unit, an amino acid residue selected from the amino acid group consisting of serine, tyrosine and cysteine. The culture medium is suitable for the high level production of a protein by an animal cell.
摘要翻译： 公开了一种用于培养动物细胞的培养基，其特征在于含有包含选自丝氨酸，酪氨酸和半胱氨酸的氨基酸的氨基酸残基作为构成单元的肽。 培养基适合于动物细胞高水平生产蛋白质。
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公开(公告)号：EP4406615A2
公开(公告)日：2024-07-31
申请号：EP24154516.9
申请日：2010-10-25
发明人： FRANZE, Reinhard , HIRASHIMA, Chikashi , LINK, Thomas , TAKAGI, Yoshinori , TAKUMA, Shinya , TSUDA, Yuriko
IPC分类号： A61P43/00
CPC分类号： C07K16/2866 , C07K2317/1420130101 , C07K2317/2420130101 , C07K2317/4120130101 , A61P43/00
摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.
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公开(公告)号：EP3202785A1
公开(公告)日：2017-08-09
申请号：EP17155939.6
申请日：2010-10-25
发明人： FRANZE, Reinhard , HIRASHIMA, Chikashi , LINK, Thomas , TAKAGI, Yoshinori , TAKUMA, Shinya , TSUDA, Yuriko
IPC分类号： C07K16/00
CPC分类号： C12P21/005 , C07K16/2866 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C07K2317/76
摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.
摘要翻译： 本文报道了用于产生免疫球蛋白的方法，其包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）在培养基中培养真核细胞，其中培养基中可获得的葡萄糖的量 每时间单位保持恒定并且限制为小于每单位时间培养基中细胞可以最大限度利用的量的80％，以及c）从培养物中回收免疫球蛋白。
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公开(公告)号：EP2154244A1
公开(公告)日：2010-02-17
申请号：EP08752120.9
申请日：2008-04-25
发明人： KATAYAMA, Satoshi , KISHISHITA, Shouhei , KODAIRA, Kunihiko , SADAMITSU, Makoto , TAKAGI, Yoshinori , MATSUDA, Hiroki
CPC分类号： C12N5/0018 , C07K16/2866 , C07K16/303 , C07K2317/24 , C12N2500/32 , C12N2500/33 , C12N2510/02 , C12P21/02
摘要： Methods of culturing cells capable of producing desired proteins to obtain the proteins by use of a medium from which biological components are excluded as much as possible are provided. Specifically, a culture method characterized by culturing while maintaining a specific amino acid in a culture solution at a high concentration, and a cell culture fed-batch medium for use in the method are provided.
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公开(公告)号：EP2351833B1
公开(公告)日：2018-07-18
申请号：EP09823558.3
申请日：2009-10-27
发明人： KATAYAMA, Satoshi , KISHISHITA, Shouhei , KODAIRA, Kunihiko , SADAMITSU, Makoto , TAKAGI, Yoshinori , MATSUDA, Hiroki
CPC分类号： C12P21/02 , C07K5/081 , C07K5/0812 , C07K16/00 , C07K16/303 , C07K2317/24 , C12N5/0037 , C12N2501/998 , C12N2510/02
摘要： Disclosed is a culture medium for culturing an animal cell, which is characterized by containing a peptide comprising, as a constituent unit, an amino acid residue selected from the amino acid group consisting of serine, tyrosine and cysteine. The culture medium is suitable for the high level production of a protein by an animal cell.
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公开(公告)号：EP2154244B1
公开(公告)日：2017-04-12
申请号：EP08752120.9
申请日：2008-04-25
发明人： KATAYAMA, Satoshi , KISHISHITA, Shouhei , KODAIRA, Kunihiko , SADAMITSU, Makoto , TAKAGI, Yoshinori , MATSUDA, Hiroki
CPC分类号： C12N5/0018 , C07K16/2866 , C07K16/303 , C07K2317/24 , C12N2500/32 , C12N2500/33 , C12N2510/02 , C12P21/02
摘要： Methods of culturing cells capable of producing desired proteins to obtain the proteins by use of a medium from which biological components are excluded as much as possible are provided. Specifically, a culture method characterized by culturing while maintaining a specific amino acid in a culture solution at a high concentration, and a cell culture fed-batch medium for use in the method are provided.
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