公开(公告)号：EP2493922A1

公开(公告)日：2012-09-05

申请号：EP10771093.1

申请日：2010-10-25

申请人： F.Hoffmann-La Roche AG ,  Chugai Seiyaku Kabushiki Kaisha

发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

IPC分类号： C07K16/00

CPC分类号： C12P21/005 ,  C07K16/2866 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/76

摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

摘要翻译： 本文报道了用于产生免疫球蛋白的方法，包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）培养培养基中的真核细胞，其中培养基中可用的葡萄糖量 每个时间单位保持恒定并且被限制在小于每单位培养基中的细胞可最大限度地利用的量的80％，和c）从培养物中回收免疫球蛋白。

公开(公告)号：EP2493922B1

公开(公告)日：2017-02-15

申请号：EP10771093.1

申请日：2010-10-25

申请人： F. Hoffmann-La Roche AG ,  Chugai Seiyaku Kabushiki Kaisha

发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

IPC分类号： C07K16/00 ,  C07K16/28 ,  C12P21/00

CPC分类号： C12P21/005 ,  C07K16/2866 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/76

摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

公开(公告)号：EP4406615A2

公开(公告)日：2024-07-31

申请号：EP24154516.9

申请日：2010-10-25

申请人： F. Hoffmann-La Roche AG ,  CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

IPC分类号： A61P43/00

CPC分类号： C07K16/2866 ,  C07K2317/1420130101 ,  C07K2317/2420130101 ,  C07K2317/4120130101 ,  A61P43/00

摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

公开(公告)号：EP3202785A1

公开(公告)日：2017-08-09

申请号：EP17155939.6

申请日：2010-10-25

申请人： F. Hoffmann-La Roche AG ,  Chugai Seiyaku Kabushiki Kaisha

发明人： FRANZE, Reinhard ,  HIRASHIMA, Chikashi ,  LINK, Thomas ,  TAKAGI, Yoshinori ,  TAKUMA, Shinya ,  TSUDA, Yuriko

IPC分类号： C07K16/00

CPC分类号： C12P21/005 ,  C07K16/2866 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/76

摘要： Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80 % of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

摘要翻译： 本文报道了用于产生免疫球蛋白的方法，其包括以下步骤：a）提供包含编码免疫球蛋白的核酸的真核细胞，b）在培养基中培养真核细胞，其中培养基中可获得的葡萄糖的量 每时间单位保持恒定并且限制为小于每单位时间培养基中细胞可以最大限度利用的量的80％，以及c）从培养物中回收免疫球蛋白。

公开(公告)号：EP2660328A1

公开(公告)日：2013-11-06

申请号：EP11854471.7

申请日：2011-12-28

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： KISHISHITA, Shohei ,  OKUI, Tomoko ,  SHINODA, Yasuharu ,  TAKUMA, Shinya

IPC分类号： C12P21/02 ,  C12P21/08 ,  C12R1/91

CPC分类号： C07K16/28 ,  C07K16/244 ,  C07K16/2866 ,  C07K16/303 ,  C07K2317/14 ,  C12N5/0602 ,  C12N2511/00 ,  C12N2523/00 ,  C12P21/02

摘要： While a desired protein is prepared by culturing an animal cell that produces the protein to cause the protein to be produced, level of heterogeneity components of the protein is modulated by performing the culture at a normal culture temperature for a certain period and then continuing the culture at a culture temperature lowered to 25-35°C.

摘要翻译： 虽然通过培养产生蛋白质以产生蛋白质的动物细胞制备所需的蛋白质，但是通过在正常培养温度下进行一定时间的培养，然后继续培养来调节蛋白质的异质性成分的水平 在培养温度降至25-35℃。