公开(公告)号：EP3483311A1

公开(公告)日：2019-05-15

申请号：EP18194361.4

申请日：2013-06-24

申请人： Gen9, Inc.

发明人： HUDSON, Michael, E. ,  KUNG, Li-yun A. ,  SCHINDLER, Daniel ,  ARCHER, Stephen ,  SAAEM, Ishtiaq

IPC分类号： C40B50/06 ,  C12N15/10 ,  C12N15/66

摘要： Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5' sequence flanking the 5' end of the internal sequence and a 3' flanking sequence flanking the 3' end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.

公开(公告)号：EP3074554A2

公开(公告)日：2016-10-05

申请号：EP14866712.4

申请日：2014-11-25

申请人： Gen9, Inc.

发明人： JACOBSON, Joseph ,  SCHINDLER, Daniel ,  SAAEM, Ishtiaq ,  GUIDO, Nicholas James

IPC分类号： C40B40/08 ,  C40B50/00

CPC分类号： C12N15/1093 ,  C12N15/1027 ,  C12N15/1031 ,  C12N15/66

摘要： Methods for designing and producing non-random libraries of nucleic acids are presented. In particular, synthesis of nonrandom libraries by multiplexed polynucleotide synthesis is utilized. Each library member may encode a promoter, ribosomal binding site and polypeptide.

摘要翻译： 本发明的方面涉及用于设计和生产核酸非随机文库的方法。 特别地，本发明的方面涉及通过多重多核苷酸合成来合成非随机文库。

公开(公告)号：EP3943646A1

公开(公告)日：2022-01-26

申请号：EP21168127.5

申请日：2014-11-25

申请人： Gen9, Inc.

发明人： JACOBSON, Joseph ,  SCHINDLER, Daniel ,  SAAEM, Ishtiaq ,  GUIDO, Nicholas James

IPC分类号： C40B40/08 ,  C40B50/00 ,  C12N15/10 ,  C12N15/66

摘要： Methods for designing and producing non-random libraries of nucleic acids are presented. In particular, synthesis of nonrandom libraries by multiplexed polynucleotide synthesis is utilized. Each library member may encode a promoter, ribosomal binding site and polypeptide.

公开(公告)号：EP2970361A1

公开(公告)日：2016-01-20

申请号：EP14769872.4

申请日：2014-03-13

申请人： Gen9, Inc.

发明人： JACOBSON, Joseph ,  SCHINDLER, Daniel ,  SAAEM, Ishtiaq ,  LAWTON, Scott ,  GOLDBERG, Martin J. ,  HUDSON, Michael E. ,  KUNG, Li-yun A.

IPC分类号： C07H21/00 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1031 ,  B01J2219/005 ,  B01J2219/00722 ,  C12P19/34

摘要： Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.

公开(公告)号：EP3393525A1

公开(公告)日：2018-10-31

申请号：EP16880003.5

申请日：2016-12-21

申请人： Gen9, Inc.

发明人： SAAEM, Ishtiaq

IPC分类号： A61K48/00 ,  C40B40/06 ,  C12P19/34

CPC分类号： C12N15/66 ,  C12N15/1093 ,  C40B40/06

摘要： Aspects of the disclosure relate to compositions and methods for polynucleotide assembly. In some embodiments, a terminator oligonucleotide is provided.

公开(公告)号：EP2864531B1

公开(公告)日：2018-10-24

申请号：EP13809969.2

申请日：2013-06-24

申请人： Gen9, Inc.

发明人： HUDSON, Michael, E. ,  KUNG, Li-yun A. ,  SCHINDLER, Daniel ,  ARCHER, Stephen ,  SAAEM, Ishtiaq

IPC分类号： C40B50/06 ,  C12N15/10 ,  C12N15/66

CPC分类号： C12N15/1031 ,  C12N15/1093 ,  C12N15/66 ,  C12Q2521/301 ,  C12Q2521/501

摘要： Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5′ sequence flanking the 5′ end of the internal sequence and a 3′ flanking sequence flanking the 3′ end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.

公开(公告)号：EP2864531A1

公开(公告)日：2015-04-29

申请号：EP13809969.2

申请日：2013-06-24

申请人： Gen9, Inc.

发明人： HUDSON, Michael, E. ,  KUNG, Li-jun A. ,  SCHINDLER, Daniel ,  ARCHER, Stephen ,  SAAEM, Ishtiaq

IPC分类号： C40B50/06

CPC分类号： C12N15/1031 ,  C12N15/1093 ,  C12N15/66 ,  C12Q2521/301 ,  C12Q2521/501

摘要： Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5′ sequence flanking the 5′ end of the internal sequence and a 3′ flanking sequence flanking the 3′ end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.