公开(公告)号：EP1532259A1

公开(公告)日：2005-05-25

申请号：EP03790951.2

申请日：2003-09-01

申请人： Geneart GmbH

发明人： BEER, Martin ,  OSTERRIEDER, Nikolaus ,  RUDOLPH, Jens ,  TRAPP, Sascha ,  WAGNER, Ralf

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/523 ,  C12N2710/16743

摘要： The present invention relates to animal viruses such as animal herpesviruses as a vector for diagnostic, therapeutic and prophylactic delivery of foreign genes and nucleic acids to animals, human or primary cells derived thereof, respectively. By using said viruses, the prevention and treatment of infectious, autoimmune and tumor diseases as well as allergies and genetic disorders can be treated. The present invention relates further to procedures to efficiently transduce primary human cells with recombinant animal viruses in vitro and in vivo. The invention further relates to a kit useful (i) for diagnosis and monitoring of innate and adoptive immune responses as well as (ii) for gene therapy and (iii) immunization purposes.

公开(公告)号：EP1356111A2

公开(公告)日：2003-10-29

申请号：EP01992785.4

申请日：2001-10-30

申请人： Geneart GmbH

发明人： WAGNER, Ralf ,  GRAF, Marcus

IPC分类号： C12Q1/68 ,  C12Q1/70 ,  C12N5/10 ,  C12N15/10 ,  C12N15/62

CPC分类号： C12N15/1051 ,  C12N15/1075 ,  C12N15/85 ,  C12N2840/44 ,  C12Q1/6897

摘要： The invention relates to reporter systems for RNA export, to a method for locating molecules which influence RNA export and to a technique for detecting a virus infection which is based on this method.

公开(公告)号：EP1282712A2

公开(公告)日：2003-02-12

申请号：EP01960229.1

申请日：2001-05-18

申请人： Geneart GmbH

发明人： WAGNER, Ralf ,  GRAF, Markus ,  DEML, Ludwig ,  BIELER, Kurt

IPC分类号： C12N15/48 ,  C12N7/04 ,  C12N5/10 ,  A61K48/00

CPC分类号： C12N9/1276 ,  C07K14/005 ,  C12N2740/15022 ,  C12N2740/16222

摘要： The present invention relates to synthetic gag and gagpol genes optimized for high level expression via codon optimization and the uses thereof for the efficient generation of vector particles. The invention further relates to the generation of packaging cells and vaccines based on the synthetic gag and gagpol genes.

公开(公告)号：EP1240333A2

公开(公告)日：2002-09-18

申请号：EP00987164.1

申请日：2000-11-16

申请人： Geneart GmbH,Gesellschaft für angewandte Biotechnologie ,  Shao, Yiming

发明人： SHAO, Yiming ,  WAGNER, Ralf ,  WOLF, Hans ,  GRAF, Marcus

IPC分类号： C12N15/49

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K2039/53 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322

摘要： The invention relates to a polynucleotide, comprising a nucleic acid sequence according to SEQ ID NO:1, 2 or 3, fragments or derivatives thereof, or a polynucleotide hybridised with the nucleic acid sequence according to SEQ ID NO:1, 2 or 3. The invention further relates to polypeptides coded from said nucleotide sequence, or fragment, or derivative of the nucleic acid sequence according to SEQ ID NO:1, 2 or 3. The polynucleotides and polypeptides may be used as medicaments, vaccines or diagnostics, in particular for the treatment, prophylaxis and diagnosis of HIV infections.

公开(公告)号：EP1584058A2

公开(公告)日：2005-10-12

申请号：EP03785937.8

申请日：2003-12-23

申请人： Geneart GmbH

发明人： RAAB, David ,  GRAF, Marcus ,  NOTKA, Frank ,  WAGNER, Ralf

IPC分类号： G06F19/00

CPC分类号： G06F19/20 ,  C07K14/43595 ,  C07K14/535 ,  C07K14/5443 ,  G06F19/18 ,  G06F19/22

摘要： The invention relates to a method for optimizing a nucleotide sequence for the purpose of expression of a protein on the basis of the amino acid sequence of said protein. According to the inventive method, a test sequence with m optimization positions is determined for a defined region, in which positions the codon usage is varied. The optimum codon usage on said optimization positions is determined by means of a power function and one or more codons of said optimum usage are determined as the codons of the optimized nucleotide sequence. These steps are iterated, while the codons of the optimized nucleotide sequence determined in the preceding steps remain unchanged during the subsequent iteration steps. The invention further relates to a device for carrying out said method.