公开(公告)号：EP1364038A2

公开(公告)日：2003-11-26

申请号：EP01962700.9

申请日：2001-06-01

申请人： Novartis AG ,  Novartis Pharma GmbH

发明人： JAKUBCZAK, John, Leonard ,  ROLLENCE, Michele, Lynette ,  STEWART, David, A. ,  STEVENSON, Susan, C. ,  HALLENBECK, Paul, L. ,  IDAMKANTI, Neeaja ,  KALEKO, Michael ,  SMITH, Theodore

IPC分类号： C12N15/86 ,  C12N15/34 ,  C12N5/10 ,  C07K14/075 ,  C12N7/04

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10222 ,  C12N2710/10243 ,  C12N2800/30 ,  C12N2810/405 ,  C12N2810/6018

摘要： This invention relates to mutated adenoviral fiber proteins and adenovirus particles containing such proteins. It further relates to polynucleotides encoding the proteins and vectors containing polynucleotides. It also relates to methods for making and using the adenoviral particles. With the mutated fiber proteins, the adenovirus particles no longer bind to their natural cellular receptor. They can then be 'retargeted' to a specific cell type through the addition of a ligand to the virus capsid, which causes the virus to bind to and infect such cell. Specific fiber mutations are listed, which ablate binding to the natural receptor. Adenovirus particles with certain fiber mutations were found to enhance gene transfer to and expression in liver as compared to viral particles with wild-type fiber.

公开(公告)号：EP1516055A2

公开(公告)日：2005-03-23

申请号：EP03732097.5

申请日：2003-01-24

申请人： The Scripps Research Institute ,  Novartis AG

发明人： KALEKO, Michael ,  NEMEROW, Glen, R. ,  SMITH, Theodore ,  STEVENSON, Susan, C.

IPC分类号： C12N15/63 ,  C12N15/11 ,  C12N15/85 ,  C12N7/00 ,  C12N15/00 ,  C07K14/00 ,  A23J1/00 ,  C12P21/00

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2710/10351 ,  C12N2800/30 ,  C12N2810/405 ,  C12N2810/6018

摘要： Provided are adenoviral vectors and the production of such vectors. In particular, fiber shaft modifications for efficient targeting of adenoviral vectors are provided. The fiber shaft modifications can be combined with other modifications, such as fiber knob and/or penton modifications, to produce fully ablated (detargeted) adenoviral vectors. A scale-up method for the propagation of detargeted adenoviral vectors is also provided.

公开(公告)号：EP1373501A2

公开(公告)日：2004-01-02

申请号：EP01986322.4

申请日：2001-10-05

申请人： Novartis AG ,  Novartis Pharma AG

发明人： KIM, Jin, Gwan ,  SMITH, Theodore ,  STEVENSON, Susan, C. ,  KALEKO, Michael

IPC分类号： C12N15/12 ,  C12N15/62 ,  C12N15/86 ,  C12N15/47 ,  C07K14/705 ,  A61K48/00 ,  G01N33/50

CPC分类号： C12N15/86 ,  A61K35/13 ,  A61K38/00 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/705 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/32 ,  C07K2319/73 ,  C12N15/62 ,  C12N15/87 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/405 ,  C12N2810/50 ,  C12N2810/859

摘要： The present invention relates to targeting molecules which are useful to specifically target an adenoviral particle to a desired cell type. These targeting molecules comprise a soluble adenoviral receptor domain, a trimerization domain, and a targeting ligand domain. Further provided are polynucleotides encoding such targeting molecule, expression vectors including such polynucleotides, and methods to target an adenoviral particle to a cell, as well as methods to deliver a heterologous gene selectively to a cell.