公开(公告)号：EP1301541A2

公开(公告)日：2003-04-16

申请号：EP01955340.3

申请日：2001-07-09

申请人： Novartis AG ,  Novartis-Erfindungen Verwaltungsgesellschaft m.b.H. ,  The Scripps Research Institute

发明人： NEMEROW, Glen, R. ,  LI, Erguang

IPC分类号： C07K19/00 ,  C12N15/62 ,  A61K48/00 ,  C07K16/28 ,  C07K14/525 ,  C07K14/475 ,  C07K14/485 ,  C07K14/65 ,  C12N15/861

CPC分类号： C07K14/485 ,  A61K48/00 ,  C07K14/475 ,  C07K14/525 ,  C07K14/65 ,  C07K16/081 ,  C07K2317/34 ,  C07K2317/77 ,  C07K2319/00

摘要： Methods and products for targeting delivery vectors, such as adenoviral gene delivery particles, to selected cell types are provided. The methods rely on targeting by a bifunctional molecule that specifically complexes with a protein on the vector particle surface and with targeted cell surface proteins. The targeted cell surface proteins are any that activate the phosphatidylinositol-3-OH kinases. The bifunctional molecules, compositions, kits, and methods of preparation and use of the vector/bifunctional molecules for gene therapy are provided.

公开(公告)号：EP1516055A2

公开(公告)日：2005-03-23

申请号：EP03732097.5

申请日：2003-01-24

申请人： The Scripps Research Institute ,  Novartis AG

发明人： KALEKO, Michael ,  NEMEROW, Glen, R. ,  SMITH, Theodore ,  STEVENSON, Susan, C.

IPC分类号： C12N15/63 ,  C12N15/11 ,  C12N15/85 ,  C12N7/00 ,  C12N15/00 ,  C07K14/00 ,  A23J1/00 ,  C12P21/00

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2710/10351 ,  C12N2800/30 ,  C12N2810/405 ,  C12N2810/6018

摘要： Provided are adenoviral vectors and the production of such vectors. In particular, fiber shaft modifications for efficient targeting of adenoviral vectors are provided. The fiber shaft modifications can be combined with other modifications, such as fiber knob and/or penton modifications, to produce fully ablated (detargeted) adenoviral vectors. A scale-up method for the propagation of detargeted adenoviral vectors is also provided.

公开(公告)号：EP1639116A2

公开(公告)日：2006-03-29

申请号：EP04755025.6

申请日：2004-06-10

申请人： THE SCRIPPS RESEARCH INSTITUTE

发明人： NEMEROW, Glen, R. ,  WU, Eugene ,  STEWART, Phoebe

IPC分类号： C12N15/861 ,  C07K14/075 ,  C12N15/34 ,  A61K48/00

CPC分类号： C07K14/005 ,  C07K2319/01 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2810/6018

摘要： Recombinant detargeted and retargeted adenovirus viral particles and vectors are provided. In particular, modified fibers from adenoviruses that bind to coxsackie-adenovirus receptor (CAR) in vivo that contain modifications in the fiber shaft are provided. Adenovirus (Ad) particles that express such fibers exhibit reduced binding to CAR. Hence detargeted Ad particles are provides; also provided are retargeted particles.