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1.发明公开METHOD OF BREEDING LIPID-PRODUCING STRAIN AND UTILIZATION OF THE SAME 有权VERFAHREN ZURZÜCHTUNGEINES LIPIDPRODUZIERENDEN STAMMS UND NUTZUNG DAVON
公开(公告)号：EP1748068A4
公开(公告)日：2007-08-08
申请号：EP05727395
申请日：2005-03-28
申请人： SUNTORY LTD
CPC分类号： C12P7/6472 , C12P7/6463 , C12R1/645
摘要： A method of breeding a lipid-producing strain belonging to the genus Mortierella. According to the method of breeding a lipid-producing strain which involves an expression-inhibiting step of inhibiting the expression of a specific gene in the lipid-producing strain as described above, a lipid-producing strain belonging to the genus Mortierella can be efficiently and effectively bred.
摘要翻译： 一种育种属于被孢霉属的脂质生产菌株的方法。 根据如上所述的涉及抑制脂质生产菌株中的特定基因表达的表达抑制步骤的脂质生产菌株的育种方法，属于被孢霉属的脂质生产菌株可以被有效地 有效繁殖。
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公开(公告)号：EP1666584A4
公开(公告)日：2007-01-10
申请号：EP04771982
申请日：2004-08-20
申请人： SUNTORY LTD
发明人： OCHIAI MISA , KAWASHIMA HIROSHI , SHIMIZU SAKAYU
CPC分类号： C12P7/6463 , C12N15/80
摘要： It is intended to provide a breeding method whereby a lipid-producing fungus belonging to the genus Mortierella can be effectively and efficiently bred without resort to antibiotics. Transformation is carried out with the use of an auxotroph (for example, uracil auxotroph) of a fungus belonging to the genus Mortierella as a host. More specifically speaking, a gene compensating for the auxotrophy, which is employed as a marker gene, is transferred into the host (the gene transfer step). Next, a transformant is selected by using the recovery of the auxotrophy as the marker (the selection step). As an example of the lipid-producing fungus as described above, M. aplina may be cited.
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公开(公告)号：EP1790733A4
公开(公告)日：2008-04-09
申请号：EP05780915
申请日：2005-08-24
申请人： SUNTORY LTD
发明人： UEDA MITSUYOSHI , NAKAO MASAHIRO , ASAMI MAKI , OCHIAI MISA , FUKAMI HARUKAZU
摘要： It is intended to provide a method of screening a microorganism or a protein having a useful function by using a microchamber array and an apparatus therefor. Namely, a screening method and a screening apparatus whereby a protein having a desired enzymatic activity or a microorganism capable of producing the protein is screened from a sample to be screened by using a board which has a plural number of arrayed microchambers. According to the method and the apparatus, a protein having an enzymatic activity or a microorganism capable of producing the protein contained in a microchamber is detected by detecting fluorescence emitted by an enzymatic reaction between the protein having the enzymatic activity and a fluorescent substrate.
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公开(公告)号：EP1142998A4
公开(公告)日：2003-05-07
申请号：EP00970088
申请日：2000-10-26
申请人： SUNTORY LTD
发明人： ASHIKARI TOSHIHIKO , OCHIAI MISA
CPC分类号： C12C12/004 , C12C12/006 , C12N15/81 , C12R1/85
摘要： A DNA construct consisting of: (1) a screening marker gene; (2) a proliferation inhibitory sequence which can be induced with galactose; (3) a pair of FRT sequences located in such a direction that (1) and (2) are sandwiched between them; and (4) a DNA which can be recombined with yeast chromosomal DNAs provided in both sides of (3); characterized in that the FRT sequences contain the following sequence: 5'- GAAGTTCCTATAC TTTCTAGA GAATAGGAACTTC -3'(SEQ ID NO:1) inverted repeat spacer inverted repeat sequence (1) sequence sequence (2) or a sequence substantially identical therewith and, in each of the pairing FRT sequences, one to six bases have been deleted from the distal side of the spacer sequence in the inverted repeat sequence in the distal side of the screening marker gene and the proliferation inhibitory sequence sandwiched between them; a method of transforming a yeast belonging to the genus Saccharomyces by using the same; a yeast belonging to the genus Saccharomyces transformed thereby; and a process for producing beer characterized by using this yeast belonging to the genus Saccharomyces .
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