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公开(公告)号：EP1425586A2
公开(公告)日：2004-06-09
申请号：EP02767650.1
申请日：2002-09-16
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , SCHAFER, Jurgen , KUHN, Karsten , SCHWARZ, Josef , NEUMANN, Thomas
IPC分类号： G01N33/58
CPC分类号： G01N33/6848 , G01N33/68 , G01N33/6851 , G01N2458/15 , H01J49/04
摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectromety, and wherein the mass marker moiety comprises an amino acid and the mass normalisation moiety comprises an amino acid.
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公开(公告)号：EP1397686A1
公开(公告)日：2004-03-17
申请号：EP02735602.1
申请日：2002-06-07
发明人： HAMON, Christian , THOMPSON, Andrew , NEUMANN, Thomas , JOHNSTONE, Robert , MOHAMMED, Abdul Karim Abed
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of: (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
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公开(公告)号：EP1397505A2
公开(公告)日：2004-03-17
申请号：EP02735600.5
申请日：2002-06-07
发明人： HAMON, Christian , THOMPSON, Andrew , KUHN, Karsten , NEUMANN, Thomas , JOUBERT, Richard , JOHNSTONE, Robert , SCHMIDT, Gunter
IPC分类号： C12Q1/37 , G01N33/68 , C07D213/71 , C07D215/36 , C07D241/18 , C07D239/38 , C07D251/58 , C07K1/107
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide or a population of polypeptides, which method comprises the steps of: (a) contacting a sample comprising one or more polypeptides with a lysine reactive agent to cap ε-amino groups; (b) optionally reacting the sample of polypeptides with an amine reactive reagent to block α-amino groups; (c) digesting the sample of polypeptides with a cleavage reagent to produce peptide fragments; (d) optionally deactivating the cleavage reagent; (e) removing those peptides having uncapped or unblocked amino groups; and (f) recovering the N-terminal peptides.
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公开(公告)号：EP1490693A1
公开(公告)日：2004-12-29
申请号：EP03720676.0
申请日：2003-04-04
发明人： THOMPSON, Andrew, Hugin , HAMON, Christian , KUHN, Karsten , MEYER, Markus , JUERGEN, Schafer , NEUMANN, Thomas
CPC分类号： G01N33/6848 , C07K1/13 , G01N33/6851
摘要： Provided is a method for characterising an analyte by matrix assisted laser desorption ionisation (MALDI) mass spectrometry, which method comprises: (a) labelling the analyte with a light-absorbing label that absorbs light at a pre-determined frequency, to form a labelled analyte; (b) embedding the labelled analyte in a matrix formed from at least one compound that absorbs light, to form an embedded labelled analyte; (c) desorbing the embedded labelled analyte by exposing it to light having the pre-determined frequency, to form a desorbed analyte; and (d) detecting the desorbed analyte by mass spectrometry to characterise the analyte.
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