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1.发明授权Structural gene of membrane-bound alcohol dehydrogenase complex,plasmid containing the same and transformed acetic acid bacteria 失效膜结合乙醇脱氢酶复合物的结构基因，含有该基因的质粒，并转化乙酸菌
公开(公告)号：EP0448969B1
公开(公告)日：1996-05-15
申请号：EP91102793.6
申请日：1991-02-26
发明人： Tamaki, Toshimi , Takemura, Hiroshi , Tayama, Kenji , Fukaya, Masahiro , Okumura, Hajime , kawamura, Yoshiya
CPC分类号： C12N9/0006 , C07K16/40
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2.发明公开Fructosylamine deglycase, method of producing it, and method of quantitative determination of amadori compounds using the enzyme 失效羧甲基纤维素酶，其生产方法和使用酶的定量化合物的定量方法
公开(公告)号：EP0576838A3
公开(公告)日：1994-07-06
申请号：EP93108649.0
申请日：1993-05-28
CPC分类号： C12N9/0012 , C12Q1/26
摘要： Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an α-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the ε-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.
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3.发明公开Electrophoretic medium, gel holder, slab type electrophoretic apparatus and electrophoretic gel cutter 失效电泳介质，凝胶支架，平板型电泳仪和电泳凝胶切割器
公开(公告)号：EP0555145A3
公开(公告)日：1994-01-12
申请号：EP93400269.2
申请日：1993-02-03
发明人： Fukushige, Tomoaki , Toda, Hiroko , Akano, Hirofumi , Ito, Emiko , Fukuta, Tomohiko , Fujimori, Masahiro , Kawamura, Yoshiya
IPC分类号： B01D57/02 , G01N27/447
CPC分类号： G01N27/44704 , B01D57/02 , G01N27/44747
摘要： An electrophoretic separation medium which is composed of a gel solution and contains a water-soluble polymer. A gel holder, which is provided with stopper members and thus can hold even soft or slippery gels while maintaining a fixed shape thereof. A slab type electrophoretic apparatus, which permits automatic detection of the electrophoretic state of a sample, automatic completion of the electrophoresis under optimum conditions without setting measurement conditions, permits continuous measurement of the buffer solution temperature, and permits separation and purificiation of a large quantity of an enzyme or like physiologically active substances. An electrophoretic gel cutter, which can cut even large size electrophoretic gels very accurately and safely to desired sizes and particularly for soft and readily deformable electrophoretic gels of polyacrylamide or the like without causing damage to the gel.
摘要翻译： 电泳分离介质，由凝胶溶液组成，含有水溶性聚合物。 一种凝胶固定器，其设有止动件，因此可以保持甚至柔软或光滑的凝胶，同时保持其固定的形状。 允许自动检测样品的电泳状态的板式电泳设备，在没有设定测量条件的情况下在最佳条件下自动完成电泳，允许连续测量缓冲液温度，并且允许大量的分离和纯化 酶或类似的生理活性物质。 一种电泳凝胶切割器，其可以非常精确和安全地将大尺寸电泳凝胶非常准确和安全地切割成所需尺寸，特别是用于聚丙烯酰胺等柔软易变形电泳凝胶而不会损坏凝胶。
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4.发明公开Biochemical oxygen demand analyzer, methods of analysis, microorganisms used for analysis 失效生物化学家Sauerstoffbedarfanalysator，Verfahren zur Analyze，Mikroorganismen zur Verwendung in Analyze。
公开(公告)号：EP0543407A1
公开(公告)日：1993-05-26
申请号：EP92119824.8
申请日：1992-11-20
发明人： Maeda, Shigeru , Ohki, Akira , Sato, Takeshi , Kato, Naho , Akano, Hirofumi , Kawamura, Yoshiya , Hatagaki, Keizo , Takahashi, Yasushi , Yamada, Mikio , Okumura, Hajime
CPC分类号： G01N33/1806 , C12Q1/001 , G01N33/1866 , G01N33/1893 , Y10S435/817 , Y10S435/852
摘要： The present invention relates to a BOD analyzer comprising a microbe sensor containing an oxygen electrode and a microbe membrane. The microbe membrane is made by immobilizing microorganisms belonging to the genus Klebsiella in a membrane. Specifically, the present invention relates to a BOD analyzer comprising a flow cell equipped with a microbe sensor containing an oxygen electrode and a microbe membrane; and a liquid passage which is connected with the entrance of the flow cell and which is equipped with an outlet. The microbe membrane is made by immobilizing microorganisms belonging to Klebsiella oxytoca 12092 strain in a porous hydrophilic membrane having an average pore size of 0.65-3 µm in diameter by using at least one gelating agent selected from the group consisting of arginic acid or salts thereof, agar, gellan gum, xanthane gum, gelatine, carageenan, locust bean gum, methylcellulose, pectin, and pullulan. The present invention also relates to improved methods of analyzing BOD by using the BOD analyzer.
摘要翻译： 本发明涉及一种包含含有氧电极和微生物膜的微生物传感器的BOD分析仪。 微生物膜通过将属于克雷伯菌属的微生物固定在膜中而制成。 具体而言，本发明涉及一种BOD分析装置，其包括配备有含有氧电极和微生物膜的微生物传感器的流动池; 以及与流通池的入口连接并且具有出口的液体通道。 通过使用至少一种选自藻酸或其盐的凝胶剂，将属于克雷伯氏菌12092菌株的微生物固定在直径为0.65-3μm的平均孔径为0.65-3μm的多孔亲水膜中来制备微生物膜 ，琼脂，结冷胶，黄原胶，明胶，角叉菜胶，刺槐豆胶，甲基纤维素，果胶和支链淀粉。 本发明还涉及使用BOD分析仪分析BOD的改进方法。
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5.发明公开Polysaccharides bonded to a porous carrier, process for preparing them and their use 失效结合在多孔载体上的多糖，它们的制备方法和它们的用途。
公开(公告)号：EP0625524A3
公开(公告)日：1995-08-02
申请号：EP94107506.1
申请日：1994-05-13
发明人： Okamoto, yoshio , Enomoto, Naoki , Furukawa, Sachiko , Ogasawara, Yasushi , Akano. Hirofumi , Kawamura, Yoshiya
CPC分类号： B01J20/3274 , B01J20/103 , B01J20/28004 , B01J20/28083 , B01J20/28085 , B01J20/29 , B01J20/3092 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3227 , B01J20/3246 , B01J20/3257 , B01J20/3259 , B01J20/3261 , B01J20/3263 , B01J2220/4825 , B01J2220/54 , B01J2220/58 , C07B57/00 , C08B37/00
摘要： A novel substance having a main structure represented by the following Formula:
Also disclosed are a method of producing the substance in which an oligosaccharide having a degree of polymerization from 3 to 10 is chemically bonded to a silane agent at the reducing terminal of the oligosaccharide, the oligosaccharide derivative obtained is polymerized to an average degree of polymerization from about 11 to about 500 with the action of an enzyme; and a method in which the aldehyde group present at the reducing terminal of an oligosaccharide having a degree of polymerization from 3 to 10 is oxidized with an oxidizing agent, and the saccharide chain of the oxidized product is polymerized to a degree of polymerization from about 11 to about 500 by the action of an enzyme, followed by the addition of an acid. The substance is excellent in solvent resistance and useful as a separating agent for chromatography, in particular, for the separation of chiral compounds.-
6.发明公开Structural gene of membrane-bound alcohol dehydrogenase complex,plasmid containing the same and transformed acetic acid bacteria 失效膜结合醇酒精脱乙酰壳多糖复合物的结构基因，含有它们的脂质体和转化的乙酸细菌
公开(公告)号：EP0448969A3
公开(公告)日：1992-01-22
申请号：EP91102793.6
申请日：1991-02-26
发明人： Tamaki, Toshimi , Takemura, Hiroshi , Tayama, Kenji , Fukaya, Masahiro , Okumura, Hajime , kawamura, Yoshiya
CPC分类号： C12N9/0006 , C07K16/40
摘要： There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of Fig. 3 and Fig. 4. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.
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7.发明公开Structural gene of membrane-bound alcohol dehydrogenase complex,plasmid containing the same and transformed acetic acid bacteria 失效膜结合乙醇脱氢酶复合物，含有该基因的质粒，并转化乙酸菌的结构基因。
公开(公告)号：EP0448969A2
公开(公告)日：1991-10-02
申请号：EP91102793.6
申请日：1991-02-26
发明人： Tamaki, Toshimi , Takemura, Hiroshi , Tayama, Kenji , Fukaya, Masahiro , Okumura, Hajime , kawamura, Yoshiya
CPC分类号： C12N9/0006 , C07K16/40
摘要： There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus Acetobacter represented by Acetobacter altoacetigenes and shown by the nucleotide sequence of Fig. 3 and Fig. 4. This enzyme increases the efficiency of acetic acid fermentation and may be effectively utilized for quantitative determination of alcohol.
摘要翻译： 提供了膜结合乙醇脱氢酶复合物具有约7.0千碱基的所有其从属于醋杆菌属由醋杆菌属altoacetigenes表示，并且由图3和图4的碱基序列的微生物衍生的分子大小的结构基因。 4.这种酶提高醋酸发酵的效率，并且可以有效地用于定量测定醇。
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8.发明公开Structural gene for membranebound aldehyde dehydrogenase, plasmid containing the same, acetic acid bacteria transformed with said plasmid, and acetic acid fermentation using said transformant 失效用于含有ALDEHYDE DEHYDROGENASE的膜结构基因，含有其的PLASMID，使用SAID PLASMID转化的乙酸细菌和使用SAID变换的乙酸发酵
公开(公告)号：EP0332120A3
公开(公告)日：1990-05-23
申请号：EP89103936.4
申请日：1989-03-06
发明人： Fukaya, Masahiro , Tayama, Kenji , Tamaki, Toshimi , Izumo, Haruko , Okumura, Hajime , Kawamura, Yoshiya
CPC分类号： C12N9/0008 , C12P7/54 , C12Y102/01003 , C12Y102/01004 , C12Y102/01005
摘要： This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter , said structural gene having a molecular size of about 3.6 Kb and having a restriction enzyme cleavage map as shown in Fig. 1; to a plasmid containing said structural gene; to an acetic acid bacterium transformed by said plasmid; and to an acetic acid fermentation process using said transformant.
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9.发明公开Acidic heteropolysaccharide AM-2, a process for the production thereof, uses thereof and Acetobacter MH-1597 (FERM BP-280) 失效酸性杂AM-2，处理用于其生产，其用途和醋杆菌属MH-1597（FERM BP-280）。
公开(公告)号：EP0127698A1
公开(公告)日：1984-12-12
申请号：EP83111347.7
申请日：1983-11-14
IPC分类号： C12P19/04
CPC分类号： C12R1/02 , A23L29/269 , C12P19/04 , Y10S435/823
摘要： Novel highly viscous acidic heterpolysaccharide AM-2 is produced from a strain of genus Acetobacter isolated from vinegar mash, and is useful as an adhesive, coating agent, drilling mud additive, enhanced oil recovering agent, etc., as well as a material useful for the production of foods, drugs and cosmetics because of its safety and high viscosity.
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公开(公告)号：EP0600607A3
公开(公告)日：1996-07-03
申请号：EP93308586.2
申请日：1993-10-28
IPC分类号： G01N27/42 , G01N27/416
CPC分类号： G01N27/423 , G01N33/1806
摘要： The present invention provides a rapid coulometric analysis for the quantitative determination of a sample substance with high reproducibility without any application of voltage etc. from the outside, as well as a galvanic cell and a device for use in said coulometric analysis. According to the present invention, a wide variety of components can be analyzed by selection of electroactive substance introduced into the galvanic cell. The present invention enables a simple and easy analysis for food components such as glutamic acid, ascorbic acid, etc., water quality such as COD, etc.
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