摘要:
Methods and systems are disclosed for extracting an image of a target fluorophore in a biological material, which involve inducing both autofluorescence of the biological material and fluorescence of the fluorophore, acquiring an image arising from both the autofluorescence of the biological material and the fluorophore, and an image arising only from the autofluorescence, subtracting the two images to produce an image representing only the fluorophore, wherein relative intensities of the excitation light used to induce the autofluorescence and the fluorescence are modulated prior to acquiring the images.
摘要:
A filter includes a central filter region, the central filter region to transmit a first wavelength range, a peripheral filter region, the peripheral filter region to block a second wavelength range, and a transition filter region between the central and peripheral filter regions, the transition filter region to transmit or block the second wavelength range differently than the second wavelength range is to be transmitted or blocked in the central and peripheral filter regions. More generally, there may be “N” regions and up to N-1 transition regions.
摘要:
Vessel perfusion and myocardial blush are determined by analyzing fluorescence signals obtained in a static region-of-interest (ROI) in a collection of fluorescence images of myocardial tissue. The blush value is determined from the total intensity of the intensity values of image elements located within the smallest contiguous range of image intensity values containing a predefined fraction of a total measured image intensity of all image elements within the ROI. Vessel (arterial) peak intensity is determined from image elements located within the ROI that have the smallest contiguous range of highest measured image intensity values and contain a predefined fraction of a total measured image intensity of all image elements within the ROI. Also disclosed is a method for tracking a moving blood vessel to aid in assessing peak vessel intensity over time. Improvement in cardiac function can be established by comparing the time differential between the time of peak intensity in a blood vessel and that in a region of neighboring myocardial tissue both pre and post procedure.
摘要:
Disclosed herein are methods, kits, and compositions for medical imaging relating to fluorescent dyes entrapped in erythrocytes. Also disclosed therein are methods, and compositions further comprising erythrocytes entrapping at least one therapeutically active agent.
摘要:
An apparatus for providing a light output to an optical guide for illumination of an imaged object including a plurality of solid state light-emitting sources each of which are independently powered and independently controlled, each light-emitting source emitting light at a wavelength which is different from the wavelength emitted by the other light-emitting sources. The apparatus also includes a heat sink configured to thermally couple the plurality of solid state light-emitting sources and provide conduction of heat generated by the plurality of solid state light-emitting sources. The apparatus further includes an optical elements to collect, collimate, and combine the emissions from the plurality of solid state light-emitting sources into a combined beam of light to be optically coupled to the light guide.
摘要:
A system for generating multi-wavelength fluorescence and reflectance images includes a single multi-mode light source for producing both multi-wavelength excitation light for fluorescence imaging and illumination light having red, green and blue components, light source filters positioned stationarily during an imaging mode and transmitting substantially all the multi-wavelength excitation light intensity and selectively transmitting a predetermined portion of one or more of the red, green and blue component intensity. A camera receiving light collected from a tissue sample includes two color image sensors, with spectral filters positioned in front of the color image sensors. The corresponding filters block excitation light and transmit at the first color image sensor reflectance light at wavelengths other than the excitation light, and transmit at the second color image sensor multi-wavelength fluorescence light at wavelengths other than the multi-wavelength excitation light.
摘要:
A method and an apparatus for preoperative identification of a perforator vessel for plastic and/or reconstructive surgery using ICG fluorescence angiography imaging are disclosed. Time-resolved image processing is used to highlight perforator locations and to enable visual discrimination among candidate perforators by various computed metrics Based on these metrics, the surgeon is able to interactively locate and select perforator vessels suitable for plastic and reconstructive surgery