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1.发明授权
公开(公告)号：EP0688866B1
公开(公告)日：1998-09-09
申请号：EP95304425.2
申请日：1995-06-23
CPC分类号： C12Y302/01141 , A61K8/60 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N9/2402 , C12P19/12 , C12P19/14 , Y10S435/822
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公开(公告)号：EP0628630B1
公开(公告)日：1998-05-06
申请号：EP94303969.3
申请日：1994-06-02
CPC分类号： C13K13/00 , A21D2/181 , A21D2/186 , A21D8/042 , A23C9/005 , A23C9/1307 , A23C9/156 , A23G1/40 , A23G3/346 , A23G3/42 , A23G4/10 , A23G2200/02 , A23G2200/06 , A23L2/39 , A23L9/12 , A23L11/05 , A23L13/428 , A23L13/72 , A23L15/20 , A23L15/30 , A23L17/70 , A23L27/33 , A23L29/35 , A61K8/60 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C12G3/02 , C12N9/2457 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/24 , C12Y302/01141
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3.发明公开Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide 失效从非还原性糖重组的热稳定酶海藻糖释放
公开(公告)号：EP0697461A1
公开(公告)日：1996-02-21
申请号：EP95305100.0
申请日：1995-07-21
CPC分类号： C12Y302/01141 , C12N9/1051 , C12P19/12 , C12P19/14 , C12Y204/01064 , Y10S435/822 , Y10S530/825
摘要： Disclosed is a recombinant thermostable enzyme which has a molecular weight of about 54,000-64,000 daltons and a pI of about 5.6-6.6, and releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3. The enzyme has a satisfactorily-high thermostability, i.e. it is not substantially inactivated even when incubated in an aqueous solution (pH 7.0) at 85°C for 60 min, and this facilitates the production of trehalose on an industrial scale and in a satisfactorily-high yield.
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4.发明公开Thermostable trehalose-releasing enzyme, and its preparation and uses 失效Thermostabile Trehalose-freisetzendes Enzym，seine Herstellung und Verwendung
公开(公告)号：EP0688866A1
公开(公告)日：1995-12-27
申请号：EP95304425.2
申请日：1995-06-23
CPC分类号： C12Y302/01141 , A61K8/60 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N9/2402 , C12P19/12 , C12P19/14 , Y10S435/822
摘要： Disclosed is a thermostable trehalose-releasing enzyme, and its preparations and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of hydrolyzing at a temperature of over 55°C the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. Trehalose and compositions containing the same are extensively useful in food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了一种耐热海藻糖释放酶及其制备和用途。 该酶可从微生物的培养物获得，例如硫酸亚硫酸杆菌（ATCC 33909和ATCC 49426）和溶血性硫磺（Sulfolobus solfataricus）（ATCC 35091和ATCC 35092），并且能够在超过55℃的温度下水解海藻糖部分和 具有海藻糖结构作为末端单元且具有3或更高葡萄糖聚合度的非还原性糖中的剩余糖基部分。 海藻糖和含有它们的组合物在食品，化妆品和药物中广泛使用。
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5.发明公开Trehalose releasing enzyme, DNA encoding therefor, their preparation and uses 失效Trehalose-freisetzendes Enzym，dafürkodierende DNA，Herstellung und Verwendung。
公开(公告)号：EP0671470A2
公开(公告)日：1995-09-13
申请号：EP95301474.3
申请日：1995-03-07
CPC分类号： C12Y302/01141 , A61K38/00 , C12N9/2402 , C12P19/14 , Y02A50/473
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
摘要翻译： 公开了编码从海藻糖结构作为末端单元，葡萄糖聚合度为3以上，重组DNA，酶，转化体及其制备和用途的非还原性糖类释放海藻糖的DNA。 这些以相对容易且成本低的方式促进海藻糖的工业规模化生产，由此获得的海藻糖可以令人满意地用于各种食品，化妆品和药物。
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6.发明授权Trehalose releasing enzyme, DNA encoding therefor, their preparation and uses 失效海藻糖释放酶，编码它们的DNA，它们的制备和用途
公开(公告)号：EP0671470B9
公开(公告)日：2004-11-03
申请号：EP95301474.3
申请日：1995-03-07
CPC分类号： C12Y302/01141 , A61K38/00 , C12N9/2402 , C12P19/14 , Y02A50/473
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
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7.发明授权Trehalose releasing enzyme, DNA encoding therefor, their preparation and uses 失效海藻糖释放酶，DNA编码为此，制备和使用
公开(公告)号：EP0671470B1
公开(公告)日：2004-01-28
申请号：EP95301474.3
申请日：1995-03-07
CPC分类号： C12Y302/01141 , A61K38/00 , C12N9/2402 , C12P19/14 , Y02A50/473
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
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8.发明公开Trehalose releasing enzyme, DNA encoding therefor, their preparation and uses 失效海藻糖释放酶，DNA编码为此，制备和用途。
公开(公告)号：EP0671470A3
公开(公告)日：1997-02-05
申请号：EP95301474.3
申请日：1995-03-07
CPC分类号： C12Y302/01141 , A61K38/00 , C12N9/2402 , C12P19/14 , Y02A50/473
摘要： Disclosed are a DNA encoding an enzyme which releases trehalose from non-reducing saccharides having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher, recombinant DNA and enzyme, transformant, and their preparations and uses. These facilitate the industrial-scale production of trehalose with a relative easiness and low cost, and trehalose thus obtained can be satisfactorily used in a variety of food products, cosmetics and pharmaceuticals.
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9.发明授权Recombinant thermostable enzyme which releases trehalose from non-reducing saccharide 失效Rekombinante恒温器Enzym das Trehalose aus nicht reduzierendes Saccharid freisetzt
公开(公告)号：EP0697461B1
公开(公告)日：1998-09-30
申请号：EP95305100.0
申请日：1995-07-21
CPC分类号： C12Y302/01141 , C12N9/1051 , C12P19/12 , C12P19/14 , C12Y204/01064 , Y10S435/822 , Y10S530/825
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公开(公告)号：EP0628630A3
公开(公告)日：1995-06-14
申请号：EP94303969.3
申请日：1994-06-02
CPC分类号： C13K13/00 , A21D2/181 , A21D2/186 , A21D8/042 , A23C9/005 , A23C9/1307 , A23C9/156 , A23G1/40 , A23G3/346 , A23G3/42 , A23G4/10 , A23G2200/02 , A23G2200/06 , A23L2/39 , A23L9/12 , A23L11/05 , A23L13/428 , A23L13/72 , A23L15/20 , A23L15/30 , A23L17/70 , A23L27/33 , A23L29/35 , A61K8/60 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/00 , A61Q19/02 , C12G3/02 , C12N9/2457 , C12N9/90 , C12P19/12 , C12P19/14 , C12P19/24 , C12Y302/01141
摘要： Disclosed is a trehalose-releasing enzyme which specifically hydrolyzes the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. The molecular weight of the enzyme is about 57,000 to 68,000 daltons on SDS-PAGE, and the isoelectric point is about 3.3 to 4.6 on isoelectrophoresis. The enzyme is useful in an industrial-scale preparation of trehalose, and the trehalose prepared therewith can be readily incorporated into food products, as well as cosmetic- and pharmaceutical-compositions.
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