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1.发明公开
公开(公告)号：EP0990704A2
公开(公告)日：2000-04-05
申请号：EP99307220.6
申请日：1999-09-13
CPC分类号： C12P19/04 , C12N9/2402 , C12N9/90 , C12P19/14 , C12P19/18 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45°C but below 60°C; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology.
摘要翻译： 一种非还原性糖形成酶和一种海藻糖释放酶，它在中等温度范围在最适温度有，即超过40或45℃但低于60℃的温度; 和最适pH的在酸性pH，范围即pH小于7的双类型的酶可以以期望的量来获得，例如，通过在能够产生酶或的营养培养基中培养微生物 通过重组DNA技术。
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2.发明授权Alpha-glucosyl derivative of catecholamine or its salt, and its preparation and uses 失效α-葡糖基化的儿茶酚胺衍生物，它们的盐，制备方法和用途
公开(公告)号：EP0564099B1
公开(公告)日：2000-02-09
申请号：EP93301668.5
申请日：1993-03-05
发明人： Nakada, Tetsuya , Kubota, Michio
IPC分类号： C07H15/203 , C07H15/12 , A61K31/70 , C08B37/16 , A61K31/715
CPC分类号： C07H15/12 , C07H15/203 , C07H15/23 , C08B37/0015
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4.发明授权
公开(公告)号：EP0688866B1
公开(公告)日：1998-09-09
申请号：EP95304425.2
申请日：1995-06-23
CPC分类号： C12Y302/01141 , A61K8/60 , A61Q7/00 , A61Q11/00 , A61Q17/04 , A61Q19/02 , C12N9/2402 , C12P19/12 , C12P19/14 , Y10S435/822
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5.发明公开Recombinant enzyme for converting maltose into trehalose 失效DNS，死亡重组蛋白Enzym zur Umwandlung von Maltose in Trehalose kodiert
公开(公告)号：EP0695804A2
公开(公告)日：1996-02-07
申请号：EP95303933.6
申请日：1995-06-08
摘要： A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose.
摘要翻译： 重组酶在SDS-PAGE上具有约57,000-67,000道尔顿分子量，在等电泳上的pI约为4.1-5.1，将麦芽糖转化成海藻糖，反之亦然。 根据酶的条件，当作用于麦芽糖时，酶形成约70w / w％的海藻糖，而当作用于海藻糖时约20w / w％的麦芽糖。 通过向宿主引入含有编码酶的DNA和可自我复制载体的重组DNA制备的转化体的培养有利于海藻糖的工业规模生产。
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公开(公告)号：EP0289138A2
公开(公告)日：1988-11-02
申请号：EP88302736.9
申请日：1988-03-28
发明人： Kubota, Michio , Nakada, Tetsuya , Sakai, Shuzo
CPC分类号： C12N9/2417
摘要： A maltotetraose-forming amylase derived from a bacterium of the genus Pseudomonas (e.g. Pseudomonas stutzeri FERM BP-1682) has a relatively wide optimum- and stable-pH, as well as having a relatively high optimum- and stable-temperature.
摘要翻译： 衍生自假单胞菌（Pseudomonas stutzeri FERM BP-1682）的细菌的形成麦芽四糖的淀粉酶具有相对宽的最佳和稳定的pH，以及具有相对较高的最佳和稳定的温度。
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7.发明公开Alpha isomaltosylglucosaccharide synthase, process for producing the same and use thereof 有权阿尔法异麦芽糖糖合成酶，Herstellungsverfahren und Verwendung davon
公开(公告)号：EP2463378A1
公开(公告)日：2012-06-13
申请号：EP10184877.8
申请日：2001-07-25
CPC分类号： A23G1/40 , A21D2/181 , A23C9/005 , A23C9/1307 , A23C9/1544 , A23C9/156 , A23G3/42 , A23G4/10 , A23G9/34 , A23L2/60 , A23L15/00 , A23L15/20 , A23L27/30 , A23L33/10 , A23L33/21 , A61K8/60 , A61K8/66 , A61K9/0014 , A61K47/26 , A61Q11/00 , A61Q19/00 , A61Q19/02 , A61Q19/10 , C07H3/04 , C07H3/06 , C12N9/1051 , C12N9/2425 , C12N9/2428 , C12P19/04 , C12Y302/01001 , C12Y302/01002 , C12Y302/01003 , C12Y302/0102
摘要： The object of the present invention is to provide an α-isomaltosylglucosaccharide-forming enzyme, process of the same, cyclotetrasaccharide, and saccharide composition comprising the saccharide which are obtainable by using the enzyme; and is solved by establishing an α-isomaltosylglucosaccharide-forming enzyme which forms a saccharide, having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing the α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as a linkage at the non-reducing end without substantially increasing the reducing power; α-isomaltosyl-transferring method using the enzyme; method for forming α-isomaltosylglucosaccharide; process for producing a cyclotetrasaccharide having the structure of cyclo{ ® 6)-α-D-glucopyranosyl-(1 ® 3)-α-D-glucopyranosyl-(1 ® 6)-α-D-glucopyranosyl-(1 ® 3)-α-D-glucopyranosyl-(1®} using both the α-isomaltosylglucosaccharide-forming enzyme and the α-isomaltosyl-transferring enzyme; and the uses of the saccharides obtainable therewith.
摘要翻译： 本发明的目的是提供一种可以通过使用该酶得到的形成酶的方法，该方法包括：通过使用该酶获得的含有糖异麦芽糖的葡萄糖， 并且通过建立形成糖的± - 异麦芽糖基葡萄糖形成酶，其葡萄糖聚合度至少为3，并且在非还原末端具有作为连接的±-1,6糖苷键和± - 通过催化来自葡萄糖聚合度至少为2的具有±1,4糖苷键作为连接的糖的±葡萄糖基转移而在非还原末端的连接以外的1,4-葡糖苷键 非还原端不显着增加还原能力; 使用酶的± - 异麦角硫基转移法; 形成± - 异麦芽糖基糖的方法; 用于生产具有环{6}±D-吡喃葡萄糖基 - （1→3） - ±D-吡喃葡萄糖基 - （1→6） - ±D-吡喃葡萄糖基 - （1→3）结构的环状四糖的方法 - ±D-吡喃葡萄糖基 - 使用± - 异麦芽糖基葡萄糖形成酶和± - 异麦芽糖基转移酶;以及可用其获得的糖的用途。
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公开(公告)号：EP0812915B1
公开(公告)日：2008-10-22
申请号：EP97304032.2
申请日：1997-06-10
发明人： Tsusaki, Keiji , Kubota, Michio , Chaen, Hiroto
CPC分类号： C12P19/14 , C12N9/2431 , C12P19/18 , C12Y302/01026
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公开(公告)号：EP1094092B1
公开(公告)日：2006-07-26
申请号：EP00309203.8
申请日：2000-10-19
CPC分类号： C08B37/00 , C08L5/00 , Y10T428/2991 , Y10T428/8305
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10.发明授权
公开(公告)号：EP0695804B1
公开(公告)日：2004-08-18
申请号：EP95303933.6
申请日：1995-06-08
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