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    METHODS, CELLS AND ORGANISMS
    94.
    发明公开
    METHODS, CELLS AND ORGANISMS 有权
    VERFAHREN，ZELLEN UND ORGANISMEN

    公开(公告)号：EP2877571A1

    公开(公告)日：2015-06-03

    申请号：EP14772198.9

    申请日：2014-09-18

    申请人： Kymab Limited

    发明人： BRADLEY, Allan ,  ALI, Hanif ,  LEE, E-Chiang

    IPC分类号： C12N5/10 ,  C12N15/10 ,  C12N15/85 ,  C12N15/90

    CPC分类号： C12N15/102 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/052 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C12N5/0635 ,  C12N15/907 ,  C12N2510/04 ,  C12N2800/80

    摘要： The invention relates to an approach for introducing one or more desired insertions and/or deletions of known sizes into one or more predefined locations in a nucleic acid (eg, in a cell or organism genome). They developed techniques to do this either in a sequential fashion or by inserting a discrete DNA fragment of defined size into the genome precisely in a predefined location or carrying out a discrete deletion of a defined size at a precise location. The technique is based on the observation that DNA single-stranded breaks are preferentially repaired through the HDR pathway, and this reduces the chances of indels (eg, produced by NHEJ) in the present invention and thus is more efficient than prior art techniques. The invention also provides sequential insertion and/or deletions using single- or double-stranded DNA cutting.

    摘要翻译： 本发明涉及将已知大小的一种或多种所需插入和/或缺失引入核酸（例如，在细胞或生物体基因组中）的一个或多个预定义位置的方法。 他们以顺序的方式开发了这样的技术，或者通过在预定义的位置精确地将定义大小的离散的DNA片段插入到基因组中，或者在精确的位置进行定义的大小的离散的删除。 该技术基于通过HDR途径优先修复DNA单链断裂的观察，并且这降低了本发明中的indel（例如，由NHEJ产生）的机会，因此比现有技术更有效。 本发明还提供使用单链或双链DNA切割的顺序插入和/或缺失。

    CARRIER FOR GENE INTRODUCTION, GENE INTRODUCTION AGENT, METHODS FOR PRODUCING SAID CARRIER AND SAID GENE INTRODUCTION AGENT, AND METHOD FOR INTRODUCING GENE INTO CELL
    98.
    发明公开
    CARRIER FOR GENE INTRODUCTION, GENE INTRODUCTION AGENT, METHODS FOR PRODUCING SAID CARRIER AND SAID GENE INTRODUCTION AGENT, AND METHOD FOR INTRODUCING GENE INTO CELL 审中-公开
    SUPPORT FOR基因导入，GENEINFÜHRUNGSMITTEL，METHOD FOR上述夹具或上述GENEINFÜHRUNGSMITTELS和方法的对一个基因在细胞中引进的生产

    公开(公告)号：EP2857044A1

    公开(公告)日：2015-04-08

    申请号：EP13796522.4

    申请日：2013-05-30

    申请人： LSIP, LLC

    发明人： HANDA, Hiroshi ,  AKAMOTO, Satoshi ,  HATAKEYAMA, Mamoru

    IPC分类号： A61K48/00 ,  A61K9/14 ,  A61K35/76 ,  A61K47/36 ,  A61K47/48 ,  C12N15/09

    CPC分类号： C12N15/907 ,  A61K9/0019 ,  A61K9/0085 ,  A61K9/009 ,  A61K47/6901 ,  A61K47/6929 ,  C12N15/87

    摘要： The present invention provides; a novel gene introduction method which enables a gene to be introduced more safely and more freely, particularly a method for introducing a gene into a specified site in the brain safely and freely; a carrier for gene introduction use, which comprises a nano-particle and a substance capable of binding to a vector for gene introduction and has functional groups involved in the induction of phagocytosis by cells, wherein the substance capable of binding to a vector for gene introduction can bind to the surface of the nano-particle through some of the functional groups and another some of the functional groups remain unbound to the substance capable of binding to a vector for gene introduction ; and a gene introduction agent, in which a vector for gene introduction is bound to the substance capable of binding to a vector for gene introduction in the carrier for gene introduction.

    摘要翻译： 本发明提供; 其使基因的新的基因导入方法中更安全地引入和更自由，尤其是用于将基因导入在大脑安全且自由地指定部位的方法; 对于基因导入用载体，其包含纳米颗粒和物质能够结合用于基因导入的载体，并具有在被细胞吞噬作用的诱导参与官能团，worin能够结合的载体用于基因导入的物质 可以通过一些官能团和结合至纳米颗粒的表面的另一部分的官能团保持未结合到能够结合用于基因导入的载体物质; 和基因导入剂，其中，用于基因导入的载体结合到能够结合在载体基因导入的基因导入的载体的物质。