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264 条记录 (耗时 0.019 秒)

    SPATIALLY ENCODED BIOLOGICAL ASSAYS USING A MICROFLUIDIC DEVICE
    99.
    发明公开
    SPATIALLY ENCODED BIOLOGICAL ASSAYS USING A MICROFLUIDIC DEVICE 审中-公开

    公开(公告)号：EP4219745A3

    公开(公告)日：2023-12-06

    申请号：EP23156583.9

    申请日：2014-06-25

    申请人： Prognosys Biosciences, Inc.

    发明人： Chee, Mark S. ,  Routenberg, David

    IPC分类号： C12Q1/6841 ,  C12Q1/6853

    摘要： The present disclosure provides methods and assay systems for use in spatially encoded biological assays, including assays to determine a spatial pattern of abundance, expression, and/or activity of one or more biological targets across multiple sites in a sample. In particular, the present disclosure provides methods and assay systems capable of high levels of multiplexing where reagents are provided to a biological sample in order to address tag the sites to which reagents are delivered; instrumentation capable of controlled delivery of reagents, in particular, microfluidic device based instrumentation; and a decoding scheme providing a readout that is digital in nature.

    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR
    100.
    发明公开
    LIBRARY PREPARATION METHODS AND COMPOSITIONS AND USES THEREFOR 审中-公开

    公开(公告)号：EP4282982A2

    公开(公告)日：2023-11-29

    申请号：EP23192384.8

    申请日：2018-06-29

    申请人： Life Technologies Corporation

    发明人： ANDERSEN, Mark ,  MAZUR, Daniel ,  CHEN, Sihong ,  LUO, Guobin ,  PENG, Xinzhan

    IPC分类号： C12Q1/6853

    摘要： Provided are methods for preparing a library of target nucleic acid sequences, as well as compositions and uses therefor. Methods comprise contacting a nucleic acid sample with a plurality of adaptors capable of amplification of one or more target nucleic acid sequences under conditions wherein the target nucleic acid(s) undergo a first amplification; digesting the resulting first amplification products; repairing the digested target amplicons; and amplifying the repaired products in a second amplification, thereby producing a library of target nucleic acid sequence. Each of the plurality of adaptor compositions comprise a handle and a targeted nucleic acid sequence and optionally one or more tag sequences. Provided methods may be carried out in a single, addition only workflow reaction, allowing for rapid production of highly multiplexed targeted libraries, optionally including unique tag sequences. Resulting library compositions are useful for a variety of applications, including sequencing applications.