-
公开(公告)号：EP0231830A2
公开(公告)日：1987-08-12
申请号：EP87100932.0
申请日：1987-01-23
发明人： Sakata, Yoshitsugu, c/o Osaka Research Lab. , Matsuura, Shuji, c/o Osaka Research Lab. , Yamamoto, Tomoko, c/o Osaka Research Lab.
IPC分类号： G01N33/543 , G01N33/76
CPC分类号： G01N33/581 , G01N33/535 , G01N33/543
摘要： The present invention provides an extremely simple and effective method of a sandwich EIA (enzyme immunoassay) system, or more precisely, a method for the detection of an antigen or an antibody by means of a sandwich enzyme immunoassay system using a combined material of insoluble carrier/antigen/enzyme-labeled antibody or a combined material of insoluble carrier/antigen/enzyme-labeled anti-immunoglobulin-antibody which comprises an insoluble carrier having coated thereon a layer of an antibody or antigen specific to the antigen or antibody to be detected and a layer of an enzyme-labeled antibody or enzyme-labeled anto- immunoglobulin-antibody specific to the antigen or antibody to be detected. According to the method of the present invention, the immunoreaction product comprising fixed antibody/ antigen to be detected/labeled antibody or fixed antigen/ antibody to be detected/labeled antiimmunoglobulin- antibody can be formed on the surface ofthe insoluble carrier by only one incubation and only one washing operation.
摘要翻译： 本发明提供一种三明治EIA（酶免疫测定）系统的非常简单和有效的方法，或者更确切地说，通过使用不溶性载体的组合材料的夹心酶免疫测定系统检测抗原或抗体的方法 /抗原/酶标记的抗体或不溶性载体/抗原/酶标记的抗免疫球蛋白抗体的组合材料，其包含在其上涂覆抗原或抗原特异性抗体或抗体的层的不溶性载体， 的酶标记抗体或对待检测的抗原或抗体特异性的酶标记的无免疫球蛋白抗体。 根据本发明的方法，可以通过仅一次孵育在不溶性载体的表面上形成包含待检测/标记抗体的固定抗体/抗原或待检测/标记的抗免疫球蛋白抗体的固定抗原/抗体的免疫反应产物 只有一次洗涤操作。
-
公开(公告)号：EP0230273A2
公开(公告)日：1987-07-29
申请号：EP87100455.2
申请日：1987-01-15
IPC分类号： B65D81/32
CPC分类号： B65D81/3205 , Y10S215/08
摘要： This invention relates to a bottle and a method of mixing with the bottle capable of readily mixing solute or dispersoid and liquid contained in different bottles as required.
This invention employs as one bottle a glass or hard plastic bottle (12) like a Vial and as the other bottle a flexible plastic bottle (11), both bottles are engaged and fluctuated to mix the solute or dispersoid in the liquid.-
公开(公告)号：EP0193204A3
公开(公告)日：1986-12-17
申请号：EP86102657
申请日：1986-02-28
摘要： Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.
-
公开(公告)号：EP0173021A3
公开(公告)日：1986-10-08
申请号：EP85107877
申请日：1985-06-25
发明人： Sakata, Yoshitsugu , Oishi, Haruki , Hatayama, Yasumichi , Shiraishi, Hirome , Yanagisawa, Kazuya
IPC分类号： G01N33/50 , G01N33/579 , G01N21/83
CPC分类号： G01N33/579 , G01N21/83 , G01N33/50 , G01N2201/0407 , Y10S436/805
摘要： 57 Endotoxin contents in samples can be determined qualitatively or quantitatively, singly or in parallel, with high precision in a short time by a process comprising applying a light to each sample solution, measuring an initial transmitted light amount l o and a transmitted light amount at a time t, I(t), to give a ratio R(t) = l(t)/l o , and judging a gelation point by a threshold value of R(t), or further obtaining a gelation time from the gelation point. An apparatus used therefore is also disclosed.
-
公开(公告)号：EP0193204A2
公开(公告)日：1986-09-03
申请号：EP86102657.3
申请日：1986-02-28
摘要： Superoxide dismutase activity is determined by measuring hydrogen peroxide produced by using superoxide anion as a substrate and acting superoxide dismutase thereon in the presence of an electron carrier. When maleimide or a derivative thereof, and if necessary, a carbonyl compound, and if further necessary bromine ions and/or citric acid or a salt thereof are added to the electron carrier, the sensitivity and the linearity of calibration curves are further improved.
-
公开(公告)号：EP0147660A3
公开(公告)日：1986-04-09
申请号：EP84114486
申请日：1984-11-29
发明人： Yamazato, Fujio , Tokuda, Kuniaki
摘要： Disclosed herein is a high sensitivity method of colorimetrically measuring zinc by using a 2-pyridylazoaminophenol derivative represented by the following general formula (I) or a salt thereof as a coloring reagent, wherein a masking agent excellent in stability and acting specifically upon specific metals is used. As the masking agent is used a surface active agent.
wherein X and Y represent a halogen atom or a hydrogen atom, R 1 represents a hydrogen atom or a lower alkyl group, and R 2 and R 3 represent a hydrogen atom, a lower alkyl group, -(CH 2 ) n SO 3 H in which n is an integer of 1-4, or
in which ℓ is an integer of 0-4 and m is an integer of 0-4. The masking agent may be surface active agent in combination , with dimethylglyoxime in case of masking nickel and/or cobalt.-
117.发明公开Monoclonal antibody and method for quantitation of immoglobulins using the same 无效单克隆抗体和用于经由该抗体的定量测定免疫球蛋白的方法。
公开(公告)号：EP0161638A2
公开(公告)日：1985-11-21
申请号：EP85105712.5
申请日：1985-05-09
IPC分类号： C12P21/08 , C12N5/02 , C12N15/00 , G01N33/577 , G01N33/53
CPC分类号： G01N33/6854 , C07K16/4283
摘要： A monoclonal antibody to human immunoglobulins produced by a hybridoma formed by the fusion between cells derived from a mouse myeloma cell line and spleen cells of mouse immunized with human immunoglobulins and characterized by producing turbidity upon reacting with human immunoglobulins even when used alone, is used for quantitation of immunoglobulins optically.
摘要翻译： 即使当单独使用时，以通过从小鼠与人免疫球蛋白的免疫和特点的小鼠骨髓瘤细胞系和脾细胞的细胞之间的融合而形成由在与人免疫球蛋白的反应来制造的浊度的杂交瘤产生人免疫球蛋白的单克隆抗体，用于 免疫球蛋白的光学定量。
-
118.发明公开Method for preparing 8-acylthio-1,2,3,4,5,6-hexahydro-2,6-methano-3,6(e),11(a)-trimethyl-3-benzazocines 失效一种用于制备8-酰硫基-1,2,3,4,5,6-六氢-2,6-亚甲基 - 3,6-（e）中的过程，图11（a） - 三甲基-3- benzazocines。
公开(公告)号：EP0094691A1
公开(公告)日：1983-11-23
申请号：EP83104966.3
申请日：1983-05-19
发明人： Hori, Mikio , Fujimura, Hajime
IPC分类号： C07D221/26
CPC分类号： C07D401/12 , C07D221/26
摘要： A method for preparing 8-acylthio-1,2,3,4,5,6-hexahydro-2,6-methano-3,6(e), 11 (a)-trimethyl-3- benzazocines represented by general formula (I) below
wherein R represents an alkyl group, a phenyl group or a heterocyclic group, which comprises reacting 1,2,3,4,5,6-hexahydro-2,6-methano-3.6(e),11(a)-trimethyl-3-benzazocine represented by the formula (II) below
or its salt with chlorosulfonic acid, heating the reaction product in the presence of a reducing agent, and reacting the reaction mixture with an acyl halide represented by the general formula (III)-
公开(公告)号：EP0075921A1
公开(公告)日：1983-04-06
申请号：EP82108943.0
申请日：1982-09-27
发明人： Yamazato, Fujio , Tokuda, Kuniaki , Oda, Toshihiko
CPC分类号： G01N33/84
摘要： A reagent mixture for measuring magnesium comprising Xylylazo Violet I or II as a color producing agent and at least one of sulfide compounds and thioureide compounds, and if necessary one or more surface active agents, can be stored for a long period of time stably and can give a stable color development.
-
公开(公告)号：EP0067041A1
公开(公告)日：1982-12-15
申请号：EP82302865.9
申请日：1982-06-03
CPC分类号： A61B5/1495 , G01N31/22 , G01N33/84
摘要： A process for measuring the amount of calcium in a sample using as a color reagent ortho-cresolphthalein com- plexone and a buffer solution by a colorimetric method, characterized by using a carbonate and/or bicarbonate solution as buffer solution can give precise measured values without causing a problem of environmental pollution.
摘要翻译： 使用比色法测定样品中的钙量的方法，其特征在于使用碳酸盐和/或碳酸氢盐溶液作为缓冲溶液，可以得到精确的测量值，而不引起 环境污染问题
-
-
-
-
-
-
-
-
-
搜索结果
616 条记录 (耗时 0.018 秒)
