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    METHODS FOR HOMOLOGOUS RECOMBINATION
    122.
    发明授权
    METHODS FOR HOMOLOGOUS RECOMBINATION 有权
    方法进行同源重组

    公开(公告)号：EP1263972B1

    公开(公告)日：2011-09-21

    申请号：EP01918578.4

    申请日：2001-03-13

    申请人： Shire Human Genetic Therapies, Inc.

    发明人： IVANOV, Evguenii

    IPC分类号： C12N15/63

    CPC分类号： C12N15/907 ,  C07K14/4712 ,  C12N15/63

    摘要： The invention features a method of promoting an alteration at a selected site in a target DNA, e.g., in the chromosomal DNA of a cell. The method includes providing, at the site: (a) a double stranded DNA sequence which includes a selected DNA sequence; (b) an agent which enhances homologous recombination, e.g., a Rad52 protein or a functional fragment thereof; and (c) an agent which inhibits non-homologous end joining, e.g., an agent which inactivates Ku such as an anti-Ku antibody or a Ku-binding oligomer or polymer, and allowing the alteration to occur. The agent which inhibits non-homologous end joining, e.g., a Ku inactivating agent such as an anti-Ku antibody, is preferably provided locally. Component (a), (b), and (c) can be introduced together, which is preferred, or separately.

    METHOD FOR INTRODUCING MUTATED GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMAL
    123.
    发明公开
    METHOD FOR INTRODUCING MUTATED GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMAL 审中-公开
    程序中突变的GEN GEN的进口突变，大盘突变MUSTARD EADERSHIP，突变导入载体，而非人类努力敲入哺乳动物简介

    公开(公告)号：EP2363470A1

    公开(公告)日：2011-09-07

    申请号：EP09822114.6

    申请日：2009-10-23

    申请人： Fukuoka University ,  National University Corporation Kumamoto University

    发明人： HIROSE Shinichi ,  DESHIMARU Masanobu ,  ARAKI, Kimi

    IPC分类号： C12N15/09 ,  A01K67/027 ,  C12N5/10

    CPC分类号： A01K67/0275 ,  A01K2217/072 ,  A01K2227/105 ,  A01K2267/0306 ,  C07K14/705 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/107 ,  C12N2800/30

    摘要： Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting target recombinant gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutant exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced. The method enables the production of a knock-in non-human mammalian animal, such as a knock-in mouse, which carries the mutation-introduced gene.

    摘要翻译： 公开了一种用于将突变引入基因，其包括以下步骤的方法：进行到其中的突变将被引入靶基因和靶重组载体之间的同源重组的同源重组步骤中，外显子，从而代 在靶基因到其中的突变是通过在靶重组载体的靶DNA序列被引入; 和所得重组靶基因的靶DNA序列和突变导入载体的携带含有通过Cre重组酶的中间行动的突变外显子来替换突变的DNA序列的突变导入盒之间进行特异性重组的突变导入工序 通过在突变导入盒的突变的DNA，从而产生一个突变导入基因到其中的突变DNA序列已被引入靶DNA序列。 该方法能够生产敲入非人类哺乳动物的动物，检查作为敲入小鼠，其携带的突变导入基因。

    COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS
    125.
    发明公开
    COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS 有权
    ZUSAMMENSETZUNGEN ZUMVERKNÜPFENVON DNA-BINDUNGSDOMÄNENUNDSPALTUNGSDOMÄNEN

    公开(公告)号：EP2297318A1

    公开(公告)日：2011-03-23

    申请号：EP09766999.8

    申请日：2009-05-28

    申请人： Sangamo BioSciences, Inc.

    发明人： MILLER, Jeffrey, C.

    IPC分类号： C12N15/09 ,  C12N9/22

    CPC分类号： C12N9/22 ,  C07K2319/00 ,  C07K2319/80 ,  C12N15/907

    摘要： Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.

    摘要翻译： 本文公开了用于连接DNA结合结构域和切割结构域（或切割半结构域）以形成非天然存在的核酸酶的组合物。 还描述了制备和使用包含这些接头的组合物的方法。