-
11.发明公开
公开(公告)号：EP2284195A1
公开(公告)日：2011-02-16
申请号：EP10182215.3
申请日：2002-09-13
IPC分类号： C07K19/00 , C07K14/195 , C07K14/435 , C12N15/62 , C12N15/63
CPC分类号： C07K14/43595 , A01K2217/05 , C07K2319/00 , C07K2319/20 , C07K2319/60 , G01N33/542
摘要： Disclosed is a fusion protein comprising:
(a) two detection portions, wherein
(i) the first detection portion is an energy-emitting protein portion and the second detection portion is a fluorescent protein portion;
or
(ii) the two detection portions are portions of a split fluorescent protein; and
(b) a periplasmic binding protein (PBP) portion between said two detection portions, which undergoes a conformational change upon binding of a compound;
wherein said conformational change results in a change of the energy emitted by said two detection portions.摘要翻译： 公开了一种融合蛋白，其包含：（a）两个检测部分，其中（i）第一检测部分是能量发射蛋白质部分，第二检测部分是荧光蛋白质部分; 或（ii）两个检测部分是分裂荧光蛋白的部分; 和（b）所述两个检测部分之间的周质结合蛋白（PBP）部分，其在化合物结合时经历构象变化; 其中所述构象变化导致由所述两个检测部分发射的能量的变化。
-
公开(公告)号：EP2215291A1
公开(公告)日：2010-08-11
申请号：EP08835217.4
申请日：2008-10-02
IPC分类号： C30B25/02
CPC分类号： C30B29/04 , B01J2203/0695 , C30B33/02
摘要： The present invention relates to method of improving the optical properties of diamond at low pressures and more specifically to a method of producing a CVD diamond of a desired optical quality which includes growing CVD diamond and raising the temperature of the CVD diamond from about 1400 °C to about 2200 °C at a pressure of from about 1 to about 760 torr outside the diamond stability field in a reducing atmosphere for a time period of from about 5 seconds to about 3 hours.
-
公开(公告)号：EP2181328A1
公开(公告)日：2010-05-05
申请号：EP08795178.6
申请日：2008-08-08
CPC分类号： G01N33/542 , C12Q1/025 , G01N33/5038 , G01N33/66 , G01N2021/6441 , G01N2400/00
摘要： The present invention provides methods for detecting and monitoring metabolite concentrations, which comprise detection and measurement of Fluorescence Resonance Energy Transfer upon ligand binding. The methods of the present invention are useful for real time monitoring of changes in metabolite levels in living cell cultures.
-
公开(公告)号：EP1444390B1
公开(公告)日：2009-12-30
申请号：EP02797071.4
申请日：2002-11-07
CPC分类号： C30B25/105 , C30B25/12 , C30B29/04 , Y10T117/1004 , Y10T117/1008 , Y10T117/1016 , Y10T117/108
摘要： An apparatus for producing diamond in a deposition chamber including a heat-sinking holder for holding a diamond and for making thermal contact with a side surface of the diamond adjacent to an edge of a growth surface of the diamond, a noncontact temperature measurement device positioned to measure temperature of the diamond across the growth surface of the diamond and a main process controller for receiving a temperature measurement from the noncontact temperature measurement device and controlling temperature of the growth surface such that all temperature gradients across the growth surface are less than 20° C. The method for producing diamond includes positioning diamond in a holder such that a thermal contact is made with a side surface of the diamond adjacent to an edge of a growth surface of the diamond, measuring temperature of the growth surface of the diamond to generate temperature measurements, controlling temperature of the growth surface based upon the temperature measurements, and growing single-crystal diamond by microwave plasma chemical vapor deposition on the growth surface, wherein a growth rate of the diamond is greater than 1 micrometer per hour.
-
公开(公告)号：EP1933614A2
公开(公告)日：2008-06-25
申请号：EP05808452.6
申请日：2005-10-14
发明人： FROMMER, Wolf, B. , LAGER, Ida
IPC分类号： A01H1/00
CPC分类号： C07K14/195 , C07K2319/20 , C07K2319/60 , C12Q1/025 , G01N33/5044
摘要： Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.
-
16.发明公开
公开(公告)号：EP1838816A2
公开(公告)日：2007-10-03
申请号：EP06748162.2
申请日：2006-01-19
发明人： MAO, Wendy, L. , MAO, Ho-Kwang
IPC分类号： C10J3/00
CPC分类号： F17C11/005 , C01B3/0015 , Y02E60/321 , Y02E60/328
摘要： The invention relates to a method of storing hydrogen that employs a mixture of hydrogen and a hydrocarbon that can both be used as fuel. In one embodiment, the method involves maintaining a mixture including hydrogen and a hydrocarbon in the solid state at ambient pressure and a temperature in excess of about 10 K.
-
17.发明公开ULTRATOUCH CVD SINGLE CRYSTAL DIAMOND AND THREE DIMENSIONAL GROWTH THEREOF 审中-公开ULTRATOUCH CVD单晶金刚石及三维生长
公开(公告)号：EP1807346A2
公开(公告)日：2007-07-18
申请号：EP05858406.1
申请日：2005-09-09
CPC分类号： C30B25/105 , C23C16/274 , C23C16/277 , C23C16/279 , C30B29/04
摘要： The invention relates to a single-crystal diamond grown by microwave plasma chemical vapor deposition that has a toughness of at least about 30 MPa m1/2. The invention also relates to a method of producing a single-crystal diamond with a toughness of at least about 30 MPa m1/2. The invention further relates to a process for producing a single crystal CVD diamond in three dimensions on a single crystal diamond substrate.
-
公开(公告)号：EP1663866A2
公开(公告)日：2006-06-07
申请号：EP04786064.8
申请日：2004-07-14
IPC分类号： C01B31/06
CPC分类号： C30B33/02 , C01B32/25 , C23C16/274 , C23C16/277 , C23C16/279 , C30B25/105 , C30B29/04 , C30B33/00 , G11B2220/20 , G11B2220/65 , H04N5/76 , H04N5/765 , H04N5/775 , H04N5/781 , H04N9/8042 , H04N9/8205
摘要： A single crystal diamond grown by microwave plasma chemical vapor deposition annealed at pressures in excess of 4.0 GPa and heated to temperature in excess of 1500 degrees C. that has a hardness of greater than 120 GPa. A method for manufacture a hard single crystal diamond includes growing a single crystal diamond and annealing the single crystal diamond at pressures in excess of 4.0 GPa and a temperature in excess of 1500 degrees C. to have a hardness in excess of 120 GPa.
-
公开(公告)号：EP1438258A1
公开(公告)日：2004-07-21
申请号：EP02786554.2
申请日：2002-10-28
发明人： MAO, Wendy, L. , MAO, Ho-Kwang
IPC分类号： C01B3/00
CPC分类号： F17C11/005 , C01B3/001 , C01B5/00 , Y02E60/321 , Y02E60/324
摘要： A method for hydrogen storage includes providing water and hydrogen gas to a containment volume, reducing the temperature of the water and hydrogen gas to form a hydrogen clathrate at a first cryogenic temperature and a first pressure and maintaining the hydrogen calathrate at second cryogenic temperature within a temperature range of up to 250 K to effect hydrogen storage. The low-pressure hydrogen hydrate includes H2O molecules, H2 molecules and a unit cell including polyhedron cages of hydrogen-bonded frameworks of the H20 molecules built around the H2 molecules.
-
20.发明公开RNA INTERFERENCE PATHWAY GENES AS TOOLS FOR TARGETED GENETIC INTERFERENCE 审中-公开RNA干扰的方式有针对性GENTISCHEN干扰的一种工具基因
公开(公告)号：EP1235842A1
公开(公告)日：2002-09-04
申请号：EP00972167.1
申请日：2000-10-13
发明人： MELLO, Craig, C. , FIRE, Andrew , TABARA, Hiroaki , GRISHOK, Alla
IPC分类号： C07H21/04 , C07K14/435 , C07K16/18 , C12N15/63 , C12N15/67
CPC分类号： C12N15/67 , C07K14/43545
摘要： Genes involved in double-stranded RNA interference (RNAi pathway genes) are identified and used to investigate the RNAi pathway. The genes and their products are also useful for modulating RNAi pathway activity.
-
-
-
-
-
-
-
-
-
搜索结果
68 条记录 (耗时 0.028 秒)
