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11.发明公开Enzyme-immobilized electrode, composition for preparation of the same and electrically conductive enzyme 失效Immobilisierte Enzym-Elektrode，Zusammensetzung zu ihrer Herstellung und elektrischleitfähigeEnzyme。
公开(公告)号：EP0563795A1
公开(公告)日：1993-10-06
申请号：EP93104952.2
申请日：1993-03-25
CPC分类号： C12Q1/005 , C12Q1/003 , C12Q1/004 , Y10S435/817
摘要： An enzyme sensor system capable of quantitatively determining a particular component contained in a biological sample in a rapid and easy manner is disclosed. More particularly, an electrically conductive enzyme, an enzyme-immobilized electrode using the same and a composition for use in the preparation of the enzyme electrode are disclosed. The composition for an enzyme-immobilized electrode according to the present invention enables electrodes of various patterns to be produced by screen printing.
摘要翻译： 公开了能够以快速和容易的方式定量测定生物样品中所含的特定组分的酶传感器系统。 更具体地，公开了导电酶，使用该电极的酶固定电极和用于制备酶电极的组合物。 根据本发明的酶固定化电极的组合物能够通过丝网印刷制造各种图案的电极。
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公开(公告)号：EP0915174A4
公开(公告)日：2002-06-12
申请号：EP98919518
申请日：1998-05-08
发明人： KARUBE ISAO , SAWATA SHINYA , NAGATA RYOHEI
CPC分类号： C12Q1/6832 , C12Q1/686 , C12Q2531/107 , C12Q2565/501
摘要： A method for detecting a target nucleotide sequence at a high detection sensitivity with the use of a complementary nucleotide sequence. This method involves the step of converting the target nucleotide sequence into a partly double-stranded nucleotide sequence which is double-stranded in some parts but single-stranded in the others; and the step of detecting the partly double-stranded nucleotide sequence as described above with the use of the nucleotide sequence complementary to the target nucleotide sequence.
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13.发明公开Biosensitive element for surface plasmon resonance measurements, and its method of manufacture 有权生物安全性评估（Oberflächen-Plasmon-Resonanz-Messungen und dessen Herstellungsverfahren
公开(公告)号：EP0945721A2
公开(公告)日：1999-09-29
申请号：EP99105079.0
申请日：1999-03-24
IPC分类号： G01N21/55
CPC分类号： G01N21/553
摘要： An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.
摘要翻译： 本发明的目的是提供一种能够以高灵敏度检测少量目标物质的表面等离子体共振传感器的测量芯片。 本发明提供了一种用于表面等离子体共振传感器的测量芯片，其包括金属层，在所述金属层上形成的一个或多个等离子体聚合层和固定在所述等离子体聚合层的表面上的生物活性物质。
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14.发明公开
公开(公告)号：EP0915174A1
公开(公告)日：1999-05-12
申请号：EP98919518.5
申请日：1998-05-08
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/686 , C12Q2531/107 , C12Q2565/501
摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.
摘要翻译： 本发明的目的是提供使用检测灵敏度优异的互补核苷酸序列检测靶核苷酸序列的方法。 该方法包括以下步骤：将靶核苷酸序列转化为部分双链核苷酸序列，其一部分为双链，其余部分为单链，并使用互补的核苷酸序列检测所述部分双链核苷酸序列 到目标核苷酸序列。
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公开(公告)号：EP0667521A4
公开(公告)日：1997-02-05
申请号：EP94925015
申请日：1994-08-26
发明人： KARUBE ISAO , YANO KIYOKO
IPC分类号： C12Q1/00 , G01N33/18 , G01N27/327 , G01N27/416
CPC分类号： C12Q1/001 , G01N33/1806
摘要： An electrode made of a metal or carbon, a working electrode (20) in contact with this electrode and of a microorganic germ-containing thin film, a counter electrode (7), and a reference electrode (5) as necessary are immersed in an organism-containing solution (30), and the BOD of this solution is measured by determining the quantity of an electric current flowing between the working electrode (20) and counter-electrode (7) or reference electrode (5), applying a voltage between the electrodes by a potentiostat (6). By this method, the concentrations of organic compounds can be measured directly without using an oxygen electrode even when the quantity of dissolved oxygen is small.
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公开(公告)号：EP0915174B1
公开(公告)日：2007-10-10
申请号：EP98919518.5
申请日：1998-05-08
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/686 , C12Q2531/107 , C12Q2565/501
摘要： A method for detecting a target nucleotide sequence at a high detection sensitivity with the use of a complementary nucleotide sequence. This method involves the step of converting the target nucleotide sequence into a partly double-stranded nucleotide sequence which is double-stranded in some parts but single-stranded in the others; and the step of detecting the partly double-stranded nucleotide sequence as described above with the use of the nucleotide sequence complementary to the target nucleotide sequence.
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公开(公告)号：EP1560020A1
公开(公告)日：2005-08-03
申请号：EP03758985.0
申请日：2003-10-28
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： B01D57/02 , G01N27/44773 , G01N27/44791
摘要： Transfer of fluid substances, and/or substances comprised in fluid substances, is controlled by introducing a separation medium, which prevents transfer of the fluid substances, and/or the substances comprised in the fluid substances, to an intervening cavity connecting a first cavity and a second cavity; and introducing a connection medium to replace the separation medium and thereby start substance transfer to the second cavity. Substance transfer may be readily controlled without relying on mechanical means. Based on the present invention, two-dimensional electrophoretic analysis can be readily implemented on a chip.
摘要翻译： 流体物质和/或包含在流体物质中的物质的转移通过引入防止流体物质和/或包含在流体物质中的物质转移到连接第一空腔和第二空腔的居间空腔的分离介质来控制， 第二腔体; 并且引入连接介质以替换分离介质并由此开始将物质转移到第二腔。 物质转移可以容易地控制，而不依赖于机械手段。 基于本发明，可以容易地在芯片上实施二维电泳分析。
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公开(公告)号：EP1143251B1
公开(公告)日：2004-09-08
申请号：EP99900171.2
申请日：1999-01-12
申请人： EBARA CORPORATION , Karube, Isao
发明人： KARUBE, Isao , NOMURA, Yoko
IPC分类号： G01N33/543
CPC分类号： G01N33/54373
摘要： A sensor chip for surface plasmon resonance which has a thin metal film, an antibody against a substance with the steroid skeleton such as a steroid hormone, a sex hormone or an environmental hormone (excluding triazine compounds), and a fixation layer for fixing the antibody to the thin metal film; and a method for detecting an environmental hormone which involves the step of exposing the antibody in the above-mentioned sensor chip to a sample, the step of irradiating the thin metal film with light and the step of determining the intensity of the light reflected by the thin metal film. This method achieves a high sensitivity and an excellent selectivity.
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公开(公告)号：EP0950718A4
公开(公告)日：2002-05-22
申请号：EP98931022
申请日：1998-07-09
发明人： KARUBE ISAO , SAWATA SHINYA , NAGATA RYOHEI
IPC分类号： G01N33/543 , C12N15/09 , C12N15/31 , C12Q1/68 , G01N21/27 , G01N33/566 , C07K14/00
CPC分类号： C12Q1/6825 , C12Q1/6837 , C12Q2525/107
摘要： A method for detecting a target nucleotide sequence by a hybridization procedure with an extremely improved detecting sensitivity. This method involves the step of hybridizing the target nucleotide sequence with a PNA which is complementary to the whole target nucleotide sequence or a part thereof and the step of measuring the extent of the hybridization.
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公开(公告)号：EP1162454A1
公开(公告)日：2001-12-12
申请号：EP00906629.1
申请日：2000-03-02
申请人： Karube, Isao
发明人： Karube, Isao
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： B01D57/02 , G01N27/44704 , G01N27/44773
摘要： A method for two-dimensionally separating substances characterized by using separating media independently of each other for two-dimensional separation step. A clear separation image free from distortion of the separation spot is formed. The method can be applied to two-dimensional electrophoresis for proteome analysis, and hence reproducibility can be significantly improved.
摘要翻译： 一种二维分离物质的方法，其特征在于使用分离介质彼此独立地进行二维分离步骤。 形成没有分离点变形的清晰的分离图像。 该方法可以应用于蛋白质组分析的双向电泳，因此可以显着提高再现性。
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