摘要:
A drilling or oil and gas well clean up and/or fracturing method comprises providing at least one polymer-degrading enzyme comprising a polypeptide of SEQ ID NO:15, which is added to a drilling fluid used during an oil and gas well drilling operation, or to a fluid used for an oil and gas well clean up and/or fracturing operation. Combined with a second enzyme comprising a polypeptide of SEQ ID NO: 2, the enzyme comprising a polypeptide of SEQ ID NO 15 can be used to degrade a xanthan, a guar, a hydroxyalkyl guar, a carboxyalkyl guar, a guar gum, a guar gum powder, a lignified coat of guar seeds or a solidified guar gum.
摘要翻译:钻井或油气净化和/或压裂方法包括提供至少一种包含SEQ ID NO:15的多肽的聚合物降解酶,其被添加到在油气井钻井操作期间使用的钻井液中, 或用于油气清洁和/或压裂操作的流体。 与包含SEQ ID NO:2的多肽的第二种酶组合,包含SEQ ID NO 15的多肽的酶可用于降解黄原胶,瓜尔胶,羟烷基瓜尔胶,羧烷基瓜尔胶,瓜尔胶,瓜尔胶 胶粉,木瓜化的瓜尔胶种皮或固化的瓜尔胶。
摘要:
The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
摘要:
The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use are also provided. The xylanases have increased activity and stability at increased pH and temperature.