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    Polysaccharide derivatives, methods of producing them and use of them
    12.
    发明公开
    Polysaccharide derivatives, methods of producing them and use of them 失效
    引发冯·多糖，我是Herstellung und Verwendung。

    公开(公告)号：EP0644204A1

    公开(公告)日：1995-03-22

    申请号：EP94107505.3

    申请日：1994-05-13

    申请人： NAKANO VINEGAR CO., LTD.

    发明人： Okamoto, Yoshio ,  Enomoto, Naoki ,  Ohishi, Ryu ,  Ogasawara, Yasushi ,  Akano, Hirofumi ,  Kawamura, Yoshiya

    IPC分类号： C08B37/00 ,  B01J20/10 ,  C07B57/00

    CPC分类号： B01J20/3219 ,  B01J20/286 ,  B01J20/289 ,  B01J20/29 ,  B01J20/3274 ,  C07B57/00 ,  C08B33/00 ,  C08B37/00 ,  Y10S435/803

    摘要： Provided is a novel polysaccharide derivative having a main structure of the following formula (1) in which a polysaccharide or its derivative has been chemically bonded to the inner and outer surfaces of the pores of a porous carrier at the reducing terminal of the polysaccharide or polysaccharide derivative.

       Also provided are a method of producing the novel polysaccharide derivative in which a silane agent is chemically bonded to a porous carrier and thereafter a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is further chemically bonded to surface treated carrier at the reducing terminal of the polysaccharide or its derivative, and a method of producing the novel polysaccharide derivative in which a polysaccharide having a degree of polymerization from 11 to 500 or its derivative is chemically bonded to a silane agent at the reducing terminal of the polysaccharide or its derivative and thereafter the polysaccharide derivative is further chemically bonded to a porous carrier. Further provided is a separating agent for chromatography containing the novel polysaccharide derivative. The novel polysaccharide derivative has excellent solvent resistance and is useful as a separating agent for chiral resolution of chiral compounds by chromatography. The methods of the present invention efficiently give the novel polysaccharide derivative.

    摘要翻译： 本发明提供一种新型多糖衍生物，其具有下述通式（1）的主要结构，其中多糖或其衍生物在多糖或多糖的还原端已经与多孔载体的孔的内表面和外表面化学键合 衍生物。 还提供了一种制备其中硅烷试剂与多孔载体化学键合的新型多糖衍生物的方法，然后将聚合度为11〜500的多糖或其衍生物进一步化学键合到表面处理的载体 在多糖或其衍生物的还原性末端的多糖衍生物的制造方法以及多糖衍生物的制造方法，其中聚合度为11〜500的多糖或其衍生物与多糖的还原末端的硅烷化学键合 或其衍生物，此后多糖衍生物进一步化学键合到多孔载体上。 还提供了含有新型多糖衍生物的色谱分离剂。 新型多糖衍生物具有优异的耐溶剂性，可用作通过色谱手性拆分手性化合物的分离剂。 本发明的方法有效地提供了新型多糖衍生物。

    Fructosylamine deglycase, method of producing it, and method of quantitative determination of amadori compounds using the enzyme
    13.
    发明公开
    Fructosylamine deglycase, method of producing it, and method of quantitative determination of amadori compounds using the enzyme 失效
    Fruktosylamine Deglykase，它们的制备方法，用于定量测定使用该酶阿马多利组合物的方法。

    公开(公告)号：EP0576838A2

    公开(公告)日：1994-01-05

    申请号：EP93108649.0

    申请日：1993-05-28

    申请人： Nakano Vinegar Co., Ltd.

    发明人： Ishikawa, Atsushi ,  Ohshima, Yoshifumi ,  Yamada, Mikio ,  Okumura, Hajime ,  Kawamura, Yoshiya

    IPC分类号： C12N9/00 ,  G01N33/50 ,  C12N9/02

    CPC分类号： C12N9/0012 ,  C12Q1/26

    摘要： Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an α-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the ε-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.

    摘要翻译： 本发明公开了一种新颖的fructosylamines deglycase通过特异性表征，以催化化合物的氧化，以产生氢的α酮醛与胺衍生物和过氧化的阿马多利化合物; 通过培养属于念珠菌属的微生物，并具有以产生新的酶的能力产生新的酶的方法; 并且通过将新的酶向含有阿马多利样品定量测定阿马多利化合物的方法的化合物以测量过氧化氢的量，以通过氧化反应来形成，或者测量的氧的量可以通过反应从而消耗 获得从测量值的阿马多利化合物的量。 本发明提供了由高特异性为特征的与阿马多利化合物，爱尤其没有作为粘合到ε氨基，并且因此提供了一种生产所述酶的方法及定量测定的方法，其中具有糖部分的反应的新型酶 阿马多利与酶的化合物。 使用酶，阿马多利化合物的定量测定，其全部通过常规酶方法困难，能够容易。 特别地，在糖化蛋白质的从活体的样品中的量，所有这些是重要的指标物质在糖尿病的诊断的测量中，测量样品中的果糖胺的本发明的酶方法几乎不受其他干扰物质的影响 或杂质的样本中的，由它们的影响已经处于常规的化学方法是不可避免的。 量的精确的确定是可能的通过所述方法，并且该方法是从使用的染色试剂盒和仪器的不便自由。 为了测量糖血红蛋白，该方法显示另一个特性特征做它的运作需要更简单，比传统方法更少的劳动和时间。

    Enzyme sensor
    14.
    发明公开
    Enzyme sensor 失效
    Enzymsensor。

    公开(公告)号：EP0400918A1

    公开(公告)日：1990-12-05

    申请号：EP90305721.4

    申请日：1990-05-24

    申请人： NAKANO VINEGAR CO., LTD.

    发明人： Nanba, Akira ,  Fukaya, Masahiro ,  Okumura, Hajime ,  Kawamura, Yoshiya

    IPC分类号： C12M1/40 ,  G01N27/30

    CPC分类号： C12Q1/004 ,  Y10S435/817 ,  Y10S436/806

    摘要： An enzyme sensor comprising an enzyme-modified electrode and a counter electrode, wherein the enzyme-­modified electrode comprises, on an electrode substrate, a homogenous composition comprising an electron-transfer mediator and an enzyme and/or an enzyme-containing substance. The enzyme sensor is useful in analysis, such as the analysis of compounds in foods or components in the living body, the diagnosis of diseases and the control of reaction processes. The preparation of the enzyme-­modified electrode is also described.

    摘要翻译： 一种酶传感器，其包含酶修饰电极和对电极，其中所述酶修饰电极在电极基底上包含包含电子转移介质和酶和/或含酶物质的均匀组合物。 酶传感器可用于分析，如分析食品中的化合物或生物体中的成分，疾病的诊断和对反应过程的控制。 还描述了酶修饰电极的制备。

    A method for clinical examination based on the structures of immunoglobin G-linked oligosaccharides
    17.
    发明公开
    A method for clinical examination based on the structures of immunoglobin G-linked oligosaccharides 失效
    Verfahren zur klinischen Untersuchung auf Grund der Struktur von a Immunoglobin G gebundenen Oligosacchariden

    公开(公告)号：EP0698793A2

    公开(公告)日：1996-02-28

    申请号：EP95304936.8

    申请日：1995-07-14

    申请人： NAKANO VINEGAR CO., LTD.

    发明人： Ohsuga, Hiroyuki ,  Nakagawa, Hiroaki ,  Ishida, Chizu ,  Fujimori, Masahiro ,  Tsukamoto, Yoshinori ,  Takahashi, Noriko ,  Kawamura, Yoshiya ,  Mizokami, Masashi ,  Sato, Koichi ,  Yoshioka, Kazuo ,  Kibe, Tetsuya

    IPC分类号： G01N33/68 ,  G01N33/58

    CPC分类号： G01N33/6854 ,  Y10S436/824 ,  Y10T436/143333 ,  Y10T436/25375

    摘要： The present invention relates to a novel clinical examination method which comprises analyzing the alteration in the amount of a specific component in oligosaccharides of immunogloblin G. Human diseases such as liver diseases, malignant hypertension, immunogloblin A nephropathy, pediatric disorders, etc., are examined in terms of the alteration in bisecting N-acetylglucosamine-containing oligosaccharides in immunogloblin G from collected humor. The present invention provides highly accurate information in a manner applicable to practical operation for examination of liver diseases, allergic diseases, malignant hypertension, immunogloblin A nephropathy, pediatric disorders, as well as aging-dependent variations and the therapeutic effect of interferon.

    摘要翻译： 本发明涉及一种新的临床检查方法，其包括分析免疫球蛋白G的寡糖中特异性成分的量的变化。检查人类疾病如肝脏疾病，恶性高血压，免疫球蛋白A肾病，儿科疾病等 从收集幽默的免疫球蛋白G中平均分解含N-乙酰葡糖胺的寡糖的变化。 本发明以适用于肝病，过敏性疾病，恶性高血压，免疫球蛋白A肾病，儿科疾病以及老化依赖性变异和干扰素治疗效果的实际操作的方式提供高度准确的信息。